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FIG. 1. Schematic presentation of the single speciﬁc primer PCR used to clone the csp gene. A 1-kb partial fragment of the csp gene was ampliﬁed from C. sordellii NCIB10717 total DNA with KAG209 and KAG210 PCR primers, designed in highly conserved regions among Cbp, Cnp, and Cpa. Using a HindIII DNA library of C. sordellii NCIB10717 as a template, PCR was conducted with mF2, located on pKF3, and with KAG212 or KAG213, located on the known 1-kb fragment. Nucleotide sequences of the upstream, the known 1-kb, and the downstream DNA fragments showed an open reading frame of Csp. The thick arrowed line depicts the csp gene. The ﬁlled and open areas indicate nucleotide sequence-determined (known) and nondetermined (unknown) regions, respectively.
上述做了标记的部分看不懂~~什么是HindIII DNA库，mF2位于质粒pKF3上面，和PCR有什么关系~~
求解释~~

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1楼 2012-03-14 09:20:21

已阅回复此楼关注TA 给TA发消息送TA红花 TA的回帖

happywyh

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楼: Originally posted by latitudes at 2012-03-14 11:29:55:
用 HindIII 切割 C. sordellii NCIB10717构建的 DNA文库作为模板

mF2应该是质粒pKF3上的一段序列，在这作PCR上游引物用，配合下游的KAG212或KAG213引物

mF2位于质粒上，怎么可以做PCR上游引物？
引物一般是根据目的基因设计的呀？