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hqq_87

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[求助] 蛋白质二硫键打开

请问有谁做过用硼氢化钠得变性BSA吗?这个过程要注意些什么?怎样看是否得到-SH。我做了几次,感觉不对,请告诉赐教,谢谢!
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sally208

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【答案】应助回帖

感谢参与,应助指数 +1

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2楼2012-02-28 14:52:54
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hqq_87

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引用回帖:
楼: Originally posted by sally208 at 2012-02-28 14:52:54:
http://pubs.acs.org/doi/abs/10.1021/ac1032403

谢谢你的回答,这篇文章我有!请问你有没有做过,是用这篇文献还原方法好还是用硼氢化钠好?我变性之后应用,效果很差,我想初步确定是用量问题还是我根本没有变性成功,请问能不能知道,谢谢!
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3楼2012-02-28 14:58:35
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sally208

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hqq_87(金币+3): ★★★很有帮助 谢谢指导,非常感谢! 2012-02-29 16:21:35
As I know, there are some SH- reactive dyes available, which can possibly used to check whether you got free thiols after the denaturation.

You can check it by google with the phrase: 'thiol reactive dyes'...

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4楼2012-02-29 16:07:16
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hqq_87

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楼: Originally posted by sally208 at 2012-02-29 16:07:16:
As I know, there are some SH- reactive dyes available, which can possibly used to check whether you got free thiols after the denaturation.

You can check it by google with the phrase: 'thiol re ...

Thank you very much!
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5楼2012-02-29 16:22:06
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