铜虫 (著名写手)
不让吃就去死 ![]()
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【答案】应助回帖
amisking(金币+4, MolEPI+1): 鼓励积极应助！ 2012-02-15 20:24:08
boluomi(金币+5): ★★★很有帮助 2012-02-16 10:14:53
序列
>1IL8:A|PDBID|CHAIN|SEQUENCE
SAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKENWVQRVVEKFLKRAENS
>1IL8:B|PDBID|CHAIN|SEQUENCE
SAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKENWVQRVVEKFLKRAENS
Number of amino acids: 144
Molecular weight: 16753.5
Theoretical pI: 9.13
Amino acid composition:
Ala (A) 6 4.2%
Arg (R) 10 6.9%
Asn (N) 6 4.2%
Asp (D) 4 2.8%
Cys (C) 8 5.6%
Gln (Q) 4 2.8%
Glu (E) 16 11.1%
Gly (G) 4 2.8%
His (H) 4 2.8%
Ile (I) 10 6.9%
Leu (L) 12 8.3%
Lys (K) 18 12.5%
Met (M) 0 0.0%
Phe (F) 6 4.2%
Pro (P) 8 5.6%
Ser (S) 10 6.9%
Thr (T) 4 2.8%
Trp (W) 2 1.4%
Tyr (Y) 2 1.4%
Val (V) 10 6.9%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%
(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 20
Total number of positively charged residues (Arg + Lys): 28
Atomic composition:
Carbon C 744
Hydrogen H 1206
Nitrogen N 212
Oxygen O 211
Sulfur S 8
Formula: C744H1206N212O211S8
Total number of atoms: 2381
Extinction coefficients:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 14480
Abs 0.1% (=1 g/l) 0.864, assuming all pairs of Cys residues form cystines
Ext. coefficient 13980
Abs 0.1% (=1 g/l) 0.834, assuming all Cys residues are reduced
Estimated half-life:
The N-terminal of the sequence considered is S (Ser).
The estimated half-life is: 1.9 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 26.99
This classifies the protein as stable.
Aliphatic index: 83.89
Grand average of hydropathicity (GRAVY): -0.572
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