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wangjun007

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[求助] 介孔硅药物释放

有谁做过介孔硅的药物释放,你们是怎么做的?我是用透析的方法,但发现有沉淀物在透析袋里,这样药物还能出来吗?
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王小青

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我想问问你最后怎么释放药物的啊?
2楼2012-12-07 16:14:37
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wangjun007

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引用回帖:
2楼: Originally posted by 王小青 at 2012-12-07 16:14:37
我想问问你最后怎么释放药物的啊?

药物从透析袋里出来,测释放介质溶液吸收
3楼2012-12-08 11:47:56
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王小青

银虫 (初入文坛)

引用回帖:
3楼: Originally posted by wangjun007 at 2012-12-08 11:47:56
药物从透析袋里出来,测释放介质溶液吸收...

那你能说说具体步骤吗?  我现在也做药物释放  不会做
4楼2012-12-10 14:58:39
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wangjun007

金虫 (正式写手)

引用回帖:
4楼: Originally posted by 王小青 at 2012-12-10 14:58:39
那你能说说具体步骤吗?  我现在也做药物释放  不会做...

For the pH dependent release studies, three different buffer
solutions with the same NaCl concentration (0.15 M) were
chosen: sodium acetate buffer for pH 5.0, phosphate buffer for
pH 6.5 and 7.4. DOX@M-MSN–P(NIPAM-co-MAA) was
dispersed in 2 mL of buffer, and the dispersion was transferred
into a dialysis bag (molecular weight cut off ¼ 14 000). The
dialysis bag was then kept in 200 mL of buffer and gently shaken
at 37 C. At timed intervals, 1 mL of solution was withdrawn
from the solution and analyzed by UV-vis. To keep a constant
volume, 1 mL of fresh buffer was added after each sampling. All
drug release results were averaged with three measurements,其实文献挺多的,大致就是这么个方法,你要根据具体情况而定
5楼2012-12-11 11:49:52
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王小青

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引用回帖:
5楼: Originally posted by wangjun007 at 2012-12-11 11:49:52
For the pH dependent release studies, three different buffer
solutions with the same NaCl concentration (0.15 M) were
chosen: sodium acetate buffer for pH 5.0, phosphate buffer for
pH 6.5 and 7.4 ...

哦  谢谢喽
6楼2012-12-11 14:48:56
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王小青

银虫 (初入文坛)

引用回帖:
5楼: Originally posted by wangjun007 at 2012-12-11 11:49:52
For the pH dependent release studies, three different buffer
solutions with the same NaCl concentration (0.15 M) were
chosen: sodium acetate buffer for pH 5.0, phosphate buffer for
pH 6.5 and 7.4 ...

再问一下哈  最后你那个药物释放成功了吗?
7楼2012-12-11 14:50:31
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wangjun007

金虫 (正式写手)

引用回帖:
7楼: Originally posted by 王小青 at 2012-12-11 14:50:31
再问一下哈  最后你那个药物释放成功了吗?...

还行
8楼2012-12-12 11:50:56
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