[交流] 虫友们做蛋白结晶用什么方法呢

楼: Originally posted by nach at 2011-12-12 18:32:56:
hanging drop
hampton 24 holes
never recycle used plates nor coverslips

楼: Originally posted by 月月大可 at 2011-12-12 19:43:21:
悬滴法适合与优化晶体时候使用，有利于晶体长的更大。
对于初始期的条件筛选建议使用坐滴法，适合大规模条件筛选，简单快速。当初步获得晶体后根据晶体形状和用X光照照情况确定生长条件，可以考虑进一步提高蛋白 ...

5楼: Originally posted by puluo-880312 at 2011-12-13 15:42:10:
那你们用的是哪个公司的晶体板和玻璃片啊，价格如何啊

4楼: Originally posted by puluo-880312 at 2011-12-13 08:40:24:
那一块板多少钱啊，我在网上看的很贵。这样代价不是很高

楼: Originally posted by nach at 2011-12-13 16:34:06:
2.8 dollars per plate plus institut discount, it's not expensive.
Plus, for the experiment, it's worthy to do so

楼: Originally posted by 月月大可 at 2011-12-13 16:20:57:
板子好像是hampton，玻璃片最初买的都是普通盖玻片自己硅化。后来闲太麻烦从一个国产的公司买的。
所有东西都是实验室的负责采购与管理的老师订的，我不知道价格。

9楼: Originally posted by puluo-880312 at 2011-12-13 16:54:42:
呵呵。我是我们这边第一个做晶体的，所以什么都要问啊。你们应该做这方面有一段时间了吧

楼: Originally posted by 月月大可 at 2011-12-13 17:10:38:
我曾经待过的课题组是国内结构生物学的鼻祖级人物，做蛋白质晶体学超过30年。

楼: Originally posted by nach at 2011-12-12 18:32:56:
hanging drop
hampton 24 holes
never recycle used plates nor coverslips

14楼: Originally posted by puluo-880312 at 2011-12-16 05:29:50:
您好，我想单独买聚乙二醇，请问哪个公司的好一些呢，最好是那个公司有聚乙二醇3350

楼: Originally posted by nach at 2011-12-16 15:50:43:
i think from hampton is not bad, their products are pure enough for crystallo but expensive of course.

19楼: Originally posted by zhuoer1988 at 2012-07-29 15:07:27
请问悬滴法的英文是drop plate吗？