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1.Absence of never growth factor and comparison of tyrosine kinase receptor A levels in mature spermatozoa from oligoasthenozoospermic, asthenozoospermic and fertile men. 2.Expression of brain-derived neurotrophic factor on mature spermatozoa from fertile and infertile men. 3.Detection of nerve growth factor (NGF) and its specific receptor (TrkA) in ejaculated bovine sperm, and the effects of NGF on sperm function. 4.The expression and putative role of brain-derived neurotrophic factor and its receptor in bovine sperm. 5.Detection of the SRY transcript and protein in bovine ejaculated spermatozoa. ·Ç³£¸Ðл´ó¼Ò¡£ ÓÊÏ䣺llcjj158@163.com |
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µÚ¶þƪ£ºÕæµÄû²éµ½£¡¿âÀïҲûÓУ¬ÄãÈ·¶¨ÊÇSCI¼ìË÷µÄÂ𣿠µÚÈýƪ£º Detection of nerve growth factor (NGF) and its specific receptor (TrkA) in ejaculated bovine sperm, and the effects of NGF on sperm function ×÷Õß: Li, C (Li, C.)1,2; Sun, Y (Sun, Y.)1,2,3; Yi, K (Yi, K.)4; Ma, Y (Ma, Y.)1,2; Sun, Y (Sun, Y.)1,2; Zhang, W (Zhang, W.)1,2; Zhou, X (Zhou, X.)1,2 À´Ô´³ö°æÎï: THERIOGENOLOGY ¾í: 74 ÆÚ: 9 Ò³: 1615-1622 DOI: 10.1016/j.theriogenology.2010.06.033 ³ö°æÄê: DEC 2010 ±»ÒýƵ´Î: 0 (À´×Ô Web of Science) ÒýÓõIJο¼ÎÄÏ×: 39 [ ²é¿´ Related Records ] ÒýÖ¤¹ØÏµÍ¼ ÕªÒª: The objective was to confirm the presence of nerve growth factor (NGF) and its specific receptor TrkA, in ejaculated bovine sperm, and to investigate the effects of NGF on specific aspects of bovine sperm function Both TrkA transcripts and immunoreactivity typical of the translated protein were detected by RT-PCR and western blotting However only the NGF protein was detected in bovine sperm using western blotting and there was no RT PCR evidence for NGF transcripts in sperm Using an immunofluorescent technique NGF immunoreactivity was localized to the sperm head and tail whereas that of TrkA was detected in the acrosomal cap nucleus, and tail regions When sperm were treated with exogenous NGF both leptin secretion and sperm viability were increased (P < 0 05) moreover the percentages of late apoptotic and dead sperm were increased (P < 0 05) However NGF had no effects on insulin secretion mitochondrial activity, intracellular calcium levels, or the acrosome reaction of sperm (P > 0 05) In conclusion the presence of TrkA transcript as well as NGF and TrkA immunoreactivity were confirmed in bovine sperm Furthermore exogenous NGF had significant effects on the secretion of leptin, cell viability and sperm apoptosis This study provided strong evidence that NGF/TrkA may have roles in regulation of sperm physiology and perhaps male fertility and infertility (C) 2010 Elsevier Inc All rights reserved ÎÄÏ×ÀàÐÍ: Article ÓïÖÖ: English ×÷Õ߹ؼü´Ê: Bovine sperm; Nerve growth factor; Tyrosine kinase receptor; Expression KeyWords Plus: CALCIUM INFLUX; SPERMATOZOA; CELL; SECRETION; RNA; NEUROTROPHINS; EXPRESSION; VIABILITY; APOPTOSIS; SYSTEM ͨѶ×÷ÕßµØÖ·: Zhou, X (ͨѶ×÷Õß),Jilin Univ, Coll Anim Sci & Vet Med, 5333 Xi An Ave, Changchun 130062, Jilin Province, Peoples R China µØÖ·: 1. Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Jilin Province, Peoples R China 2. Jilin Univ, Jilin Prov Key Lab Anim Embryo Engn, Changchun 130062, Jilin Province, Peoples R China 3. Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Peoples R China 4. Hunan Inst Anim & Vet Sci, Changsha 410131, Hunan, Peoples R China »ù½ð×ÊÖúÖÂл: »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ National High Technology Research and Development Program ("863" Program) of China 2008AA101003 National Science and Technology Pillar Program of China 2008BADB2B09 Project for Science and Technology Development of Jilin Province, P R of China 20070210 Science and Technology Development Project of Changchun City 08GH08 [ÏÔʾ»ù½ð×ÊÖúÐÅÏ¢][Òþ²Ø»ù½ð×ÊÖúÐÅÏ¢] This work was supported by the National High Technology Research and Development Program ("863" Program) of China (grant No 2008AA101003), The National Science and Technology Pillar Program of China (grant No 2008BADB2B09), The Project for Science and Technology Development of Jilin Province, P R of China (grant No 20070210), and Science and Technology Development Project of Changchun City (08GH08) ³ö°æÉÌ: ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA Web of Science ·ÖÀà: Reproductive Biology; Veterinary Sciences ѧ¿ÆÀà±ð: Reproductive Biology; Veterinary Sciences IDS ºÅ: 683UB ISSN: 0093-691X ¼ìË÷ºÅ£ºWOS:000284499600012 µÚËÄÆª£º¿âÀïҲûÓУ¬ÄãÈ·¶¨ÊÇSCI¼ìË÷µÄÂ𣿠µÚÎåÆª£º Detection of the SRY Transcript and Protein in Bovine Ejaculated Spermatozoa ×÷Õß: Li, CJ (Li, Chunjin)1,2; Sun, YF (Sun, Yongfeng)1,2,4; Yi, KL (Yi, Kangle)3; Li, CJ (Li, Chengjiao)1,2; Zhu, XL (Zhu, Xiaoling)1,2; Chen, L (Chen, Lu)1,2; Zhou, X (Zhou, Xu)1,2 À´Ô´³ö°æÎï: ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES ¾í: 24 ÆÚ: 10 Ò³: 1358-1364 DOI: 10.5713/ajas.2011.11062 ³ö°æÄê: OCT 2011 ±»ÒýƵ´Î: 0 (À´×Ô Web of Science) ÒýÓõIJο¼ÎÄÏ×: 27 [ ²é¿´ Related Records ] ÒýÖ¤¹ØÏµÍ¼ ÕªÒª: The sex-determining region on the Y (SRY) gene is important in mammalian sex determination and differentiation. We report a study of the abundance of SRY gene products in bovine ejaculated. RT-PCR experiments using RNA extracted from bovine spermatozoa with SRY-specific primers yielded a 456 bp product, but the amount of SRY mRNA in sperm was lower than that in the testes (p<0.01). A protein of approximately 27 KDa was detected by western blotting. The SRY transcript was detected in the midpiece of approximately half the spermatozoa by in situ hybridization, and the SRY protein was detected in the heads of half the spermatozoa by immunofluorescence, indicating that SRY mRNA and protein may only be present in Y-bearing spermatozoa. These results suggest that the SRY transcript and protein are present in bovine ejaculated Y-sperm. The roles of the SRY gene in spermatogenesis, sperm motility, and the sperm-oocyte interaction merit further investigation. ÎÄÏ×ÀàÐÍ: Article ÓïÖÖ: English ×÷Õ߹ؼü´Ê: SRY Gene; Bovine Spermatozoa; In situ Hybridization; Bovine KeyWords Plus: MESSENGER-RNA; MATURE SPERMATOZOA; SEX DETERMINATION; EXPRESSION; SECRETION; RECEPTOR; SPERM; MOUSE ͨѶ×÷ÕßµØÖ·: Zhou, X (ͨѶ×÷Õß),Jilin Univ, Coll Anim Sci & Vet Med, 5333 Xian Ave, Changchun 130062, Jilin Province, Peoples R China µØÖ·: 1. Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Jilin Province, Peoples R China 2. Jilin Univ, Jilin Prov Key Lab Anim Embryo Engn, Changchun 130062, Jilin Province, Peoples R China 3. Hunan Inst Anim & Vet Sci, Changsha 410131, Hunan, Peoples R China 4. Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Peoples R China µç×ÓÓʼþµØÖ·: xzhou65@vip.sina.com »ù½ð×ÊÖúÖÂл: »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ National High Technology Research and Development Program ("863" Program) of China 2008AA101003 National Science & Technology Pillar Program of China 2008BADB2B09 Project for Science and Technology Development of Jilin Province, P.R. of China 20070210 Science and Technology Development Project of Changchun City 08GH08 [ÏÔʾ»ù½ð×ÊÖúÐÅÏ¢][Òþ²Ø»ù½ð×ÊÖúÐÅÏ¢] This work was supported by the National High Technology Research and Development Program ("863" Program) of China (No.2008AA101003), the National Science & Technology Pillar Program of China (No.2008BADB2B09), and the Project for Science and Technology Development of Jilin Province, P.R. of China (No. 20070210). Science and Technology Development Project of Changchun City (08GH08). ³ö°æÉÌ: ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, ROOM 708 SAMMO SPOREX, 1638-32, SEOWON-DONG, GWANAK-GU, SEOUL 151-730, SOUTH KOREA Web of Science ·ÖÀà: Agriculture, Dairy & Animal Science ѧ¿ÆÀà±ð: Agriculture IDS ºÅ: 820BC ISSN: 1011-2367 ¼ìË÷ºÅ£ºWOS:000294879500005 |

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