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1.Absence of never growth factor and comparison of tyrosine kinase receptor A levels in mature spermatozoa from oligoasthenozoospermic, asthenozoospermic and fertile men. 2.Expression of brain-derived neurotrophic factor on mature spermatozoa from fertile and infertile men. 3.Detection of nerve growth factor (NGF) and its specific receptor (TrkA) in ejaculated bovine sperm, and the effects of NGF on sperm function. 4.The expression and putative role of brain-derived neurotrophic factor and its receptor in bovine sperm. 5.Detection of the SRY transcript and protein in bovine ejaculated spermatozoa. ·Ç³£¸Ðл´ó¼Ò¡£ ÓÊÏ䣺llcjj158@163.com |
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2Â¥2011-10-31 05:39:14
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µÚһƪ£º Absence of nerve growth factor and comparison of tyrosine kinase receptor A levels in mature spermatozoa from oligoasthenozoospermic, asthenozoospermic and fertile men ×÷Õß: Li, CJ (Li, Chunjin)1,2; Zheng, LW (Zheng, Lianwen)3; Wang, CQ (Wang, Chunqiang)1,2,4; Zhou, X (Zhou, Xu)1,2 À´Ô´³ö°æÎï: CLINICA CHIMICA ACTA ¾í: 411 ÆÚ: 19-20 Ò³: 1482-1486 DOI: 10.1016/j.cca.2010.06.002 ³ö°æÄê: OCT 9 2010 ±»ÒýƵ´Î: 2 (À´×Ô Web of Science) ÒýÓõIJο¼ÎÄÏ×: 33 [ ²é¿´ Related Records ] ÒýÖ¤¹ØÏµÍ¼ ÕªÒª: Background: Nerve growth factor (NGF) and the Tyrosine kinase receptor (TrkA) are expressed in mammalian ovaries and testes, where they are involved in oocyte maturation and spermatogenesis. We assessed the possibility that NGF and TrkA gene products are present in human spermatozoa, and explored differences in levels of NGF and TrkA in spermatozoa from oligoasthenozoospermic, asthenozoospermic and fertile men. Methods: The presence of NGF and TrkA in human spermatozoa was investigated using RT-PCR, immunofluorescence and Western blotting. Real-time PCR and ELISA were used to determine expression levels of NGF and TrkA. Results: No NGF transcripts were detected in human spermatozoa. The levels of TrkA mRNA in spermatozoa from oligoasthenozoospermic samples were significantly lower than those in spermatozoa from fertile and asthenozoospermic samples (P<0.05). NGF concentrations in seminal plasma samples from oligoasthenozoospermic men were lower than those in seminal plasma from fertile and asthenozoospermic men, but these differences were not statistically significant (P>0.05). Conclusions: The levels of both NGF protein in seminal plasma and TrkA mRNA in spermatozoa are low in samples from oligoasthenozoospermic men. (C) 2010 Elsevier B.V. All rights reserved. ÎÄÏ×ÀàÐÍ: Article ÓïÖÖ: English ×÷Õ߹ؼü´Ê: Human spermatozoa; Nerve growth factor; Tyrosine kinase receptor; Expression; Infertility KeyWords Plus: INFERTILE MEN; NEUROTROPHIN RECEPTORS; AZOOSPERMIC MEN; SERTOLI-CELL; TRK FAMILY; EXPRESSION; TESTES; OOCYTES; SEMEN; OVARY ͨѶ×÷ÕßµØÖ·: Zhou, X (ͨѶ×÷Õß),Jilin Univ, Coll Anim Sci & Vet Med, 5333 Xian Ave, Changchun 130062, Jilin Province, Peoples R China µØÖ·: 1. Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Jilin Province, Peoples R China 2. Jilin Univ, Jilin Prov Key Lab Anim Embryo Engn, Changchun 130062, Jilin Province, Peoples R China 3. Jilin Univ, Reprod Med Ctr, Hosp 2, Changchun 130062, Jilin Province, Peoples R China 4. Liaoning Med Univ, Jinzhou 121001, Liaoning Prov, Peoples R China µç×ÓÓʼþµØÖ·: xzhou65@vip.sina.com »ù½ð×ÊÖúÖÂл: »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ National High Technology Research and Development Program ("863" Program) of China 2008AA101003 National Science & Technology Pillar Program of China 2008BADB2B09 Project for Science and Technology Development of Jilin Province, PR of China 20070210 [ÏÔʾ»ù½ð×ÊÖúÐÅÏ¢][Òþ²Ø»ù½ð×ÊÖúÐÅÏ¢] This work was supported by the National High Technology Research and Development Program ("863" Program) of China (grant no. 2008AA101003), the National Science & Technology Pillar Program of China (grant no. 2008BADB2B09), and the Project for Science and Technology Development of Jilin Province, PR of China (grant no. 20070210). ³ö°æÉÌ: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS Web of Science ·ÖÀà: Medical Laboratory Technology ѧ¿ÆÀà±ð: Medical Laboratory Technology IDS ºÅ: 638PK ISSN: 0009-8981 ¼ìË÷ºÅ£ºUT WOS:000280906800014 |

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