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1.Absence of never growth factor and comparison of tyrosine kinase receptor A levels in mature spermatozoa from oligoasthenozoospermic, asthenozoospermic and fertile men.
2.Expression of brain-derived neurotrophic factor on mature spermatozoa from fertile and infertile men.
3.Detection of nerve growth factor (NGF) and its specific receptor (TrkA) in ejaculated bovine sperm, and the effects of NGF on sperm function.
4.The expression and putative role of brain-derived neurotrophic factor and its receptor in bovine sperm.
5.Detection of the SRY transcript and protein in bovine ejaculated spermatozoa.

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Absence of nerve growth factor and comparison of tyrosine kinase receptor A levels in mature spermatozoa from oligoasthenozoospermic, asthenozoospermic and fertile men  
×÷Õß: Li, CJ (Li, Chunjin)1,2; Zheng, LW (Zheng, Lianwen)3; Wang, CQ (Wang, Chunqiang)1,2,4; Zhou, X (Zhou, Xu)1,2  
À´Ô´³ö°æÎï: CLINICA CHIMICA ACTA  ¾í: 411   ÆÚ: 19-20   Ò³: 1482-1486   DOI: 10.1016/j.cca.2010.06.002   ³ö°æÄê: OCT 9 2010  
±»ÒýƵ´Î: 2 (À´×Ô Web of Science)  
ÒýÓõIJο¼ÎÄÏ×: 33 [ ²é¿´ Related Records ]     ÒýÖ¤¹ØÏµÍ¼      
ÕªÒª: Background: Nerve growth factor (NGF) and the Tyrosine kinase receptor (TrkA) are expressed in mammalian ovaries and testes, where they are involved in oocyte maturation and spermatogenesis. We assessed the possibility that NGF and TrkA gene products are present in human spermatozoa, and explored differences in levels of NGF and TrkA in spermatozoa from oligoasthenozoospermic, asthenozoospermic and fertile men.

Methods: The presence of NGF and TrkA in human spermatozoa was investigated using RT-PCR, immunofluorescence and Western blotting. Real-time PCR and ELISA were used to determine expression levels of NGF and TrkA.

Results: No NGF transcripts were detected in human spermatozoa. The levels of TrkA mRNA in spermatozoa from oligoasthenozoospermic samples were significantly lower than those in spermatozoa from fertile and asthenozoospermic samples (P<0.05). NGF concentrations in seminal plasma samples from oligoasthenozoospermic men were lower than those in seminal plasma from fertile and asthenozoospermic men, but these differences were not statistically significant (P>0.05).

Conclusions: The levels of both NGF protein in seminal plasma and TrkA mRNA in spermatozoa are low in samples from oligoasthenozoospermic men. (C) 2010 Elsevier B.V. All rights reserved.  
ÎÄÏ×ÀàÐÍ: Article  
ÓïÖÖ: English  
×÷Õ߹ؼü´Ê: Human spermatozoa; Nerve growth factor; Tyrosine kinase receptor; Expression; Infertility  
KeyWords Plus: INFERTILE MEN; NEUROTROPHIN RECEPTORS; AZOOSPERMIC MEN; SERTOLI-CELL; TRK FAMILY; EXPRESSION; TESTES; OOCYTES; SEMEN; OVARY  
ͨѶ×÷ÕßµØÖ·: Zhou, X (ͨѶ×÷Õß),Jilin Univ, Coll Anim Sci & Vet Med, 5333 Xian Ave, Changchun 130062, Jilin Province, Peoples R China  
µØÖ·:
1. Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Jilin Province, Peoples R China
2. Jilin Univ, Jilin Prov Key Lab Anim Embryo Engn, Changchun 130062, Jilin Province, Peoples R China
3. Jilin Univ, Reprod Med Ctr, Hosp 2, Changchun 130062, Jilin Province, Peoples R China
4. Liaoning Med Univ, Jinzhou 121001, Liaoning Prov, Peoples R China  
µç×ÓÓʼþµØÖ·: xzhou65@vip.sina.com  
»ù½ð×ÊÖúÖÂл:
»ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ
National High Technology Research and Development Program ("863" Program) of China  2008AA101003  
National Science & Technology Pillar Program of China  2008BADB2B09  
Project for Science and Technology Development of Jilin Province, PR of China  20070210  

[ÏÔʾ»ù½ð×ÊÖúÐÅÏ¢][Òþ²Ø»ù½ð×ÊÖúÐÅÏ¢]   

This work was supported by the National High Technology Research and Development Program ("863" Program) of China (grant no. 2008AA101003), the National Science & Technology Pillar Program of China (grant no. 2008BADB2B09), and the Project for Science and Technology Development of Jilin Province, PR of China (grant no. 20070210).

³ö°æÉÌ: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS  
Web of Science ·ÖÀà: Medical Laboratory Technology  
ѧ¿ÆÀà±ð: Medical Laboratory Technology  
IDS ºÅ: 638PK  
ISSN: 0009-8981  
¼ìË÷ºÅ£ºUT  WOS:000280906800014
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