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1.Absence of never growth factor and comparison of tyrosine kinase receptor A levels in mature spermatozoa from oligoasthenozoospermic, asthenozoospermic and fertile men.
2.Expression of brain-derived neurotrophic factor on mature spermatozoa from fertile and infertile men.
3.Detection of nerve growth factor (NGF) and its specific receptor (TrkA) in ejaculated bovine sperm, and the effects of NGF on sperm function.
4.The expression and putative role of brain-derived neurotrophic factor and its receptor in bovine sperm.
5.Detection of the SRY transcript and protein in bovine ejaculated spermatozoa.

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shy2400

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4Â¥: Originally posted by lccj158 at 2011-10-31 06:23:29:
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Absence of nerve growth factor and comparison of tyrosine kinase receptor A levels in mature spermatozoa from oligoasthenozoospermic, asthenozoospermic and fertile men  
×÷Õß: Li, CJ (Li, Chunjin)1,2; Zheng, LW (Zheng, Lianwen)3; Wang, CQ (Wang, Chunqiang)1,2,4; Zhou, X (Zhou, Xu)1,2  
À´Ô´³ö°æÎï: CLINICA CHIMICA ACTA  ¾í: 411   ÆÚ: 19-20   Ò³: 1482-1486   DOI: 10.1016/j.cca.2010.06.002   ³ö°æÄê: OCT 9 2010  
±»ÒýƵ´Î: 2 (À´×Ô Web of Science)  
ÒýÓõIJο¼ÎÄÏ×: 33 [ ²é¿´ Related Records ]     ÒýÖ¤¹ØÏµÍ¼      
ÕªÒª: Background: Nerve growth factor (NGF) and the Tyrosine kinase receptor (TrkA) are expressed in mammalian ovaries and testes, where they are involved in oocyte maturation and spermatogenesis. We assessed the possibility that NGF and TrkA gene products are present in human spermatozoa, and explored differences in levels of NGF and TrkA in spermatozoa from oligoasthenozoospermic, asthenozoospermic and fertile men.

Methods: The presence of NGF and TrkA in human spermatozoa was investigated using RT-PCR, immunofluorescence and Western blotting. Real-time PCR and ELISA were used to determine expression levels of NGF and TrkA.

Results: No NGF transcripts were detected in human spermatozoa. The levels of TrkA mRNA in spermatozoa from oligoasthenozoospermic samples were significantly lower than those in spermatozoa from fertile and asthenozoospermic samples (P<0.05). NGF concentrations in seminal plasma samples from oligoasthenozoospermic men were lower than those in seminal plasma from fertile and asthenozoospermic men, but these differences were not statistically significant (P>0.05).

Conclusions: The levels of both NGF protein in seminal plasma and TrkA mRNA in spermatozoa are low in samples from oligoasthenozoospermic men. (C) 2010 Elsevier B.V. All rights reserved.  
ÎÄÏ×ÀàÐÍ: Article  
ÓïÖÖ: English  
×÷Õ߹ؼü´Ê: Human spermatozoa; Nerve growth factor; Tyrosine kinase receptor; Expression; Infertility  
KeyWords Plus: INFERTILE MEN; NEUROTROPHIN RECEPTORS; AZOOSPERMIC MEN; SERTOLI-CELL; TRK FAMILY; EXPRESSION; TESTES; OOCYTES; SEMEN; OVARY  
ͨѶ×÷ÕßµØÖ·: Zhou, X (ͨѶ×÷Õß),Jilin Univ, Coll Anim Sci & Vet Med, 5333 Xian Ave, Changchun 130062, Jilin Province, Peoples R China  
µØÖ·:
1. Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Jilin Province, Peoples R China
2. Jilin Univ, Jilin Prov Key Lab Anim Embryo Engn, Changchun 130062, Jilin Province, Peoples R China
3. Jilin Univ, Reprod Med Ctr, Hosp 2, Changchun 130062, Jilin Province, Peoples R China
4. Liaoning Med Univ, Jinzhou 121001, Liaoning Prov, Peoples R China  
µç×ÓÓʼþµØÖ·: xzhou65@vip.sina.com  
»ù½ð×ÊÖúÖÂл:
»ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ
National High Technology Research and Development Program ("863" Program) of China  2008AA101003  
National Science & Technology Pillar Program of China  2008BADB2B09  
Project for Science and Technology Development of Jilin Province, PR of China  20070210  

[ÏÔʾ»ù½ð×ÊÖúÐÅÏ¢][Òþ²Ø»ù½ð×ÊÖúÐÅÏ¢]   

This work was supported by the National High Technology Research and Development Program ("863" Program) of China (grant no. 2008AA101003), the National Science & Technology Pillar Program of China (grant no. 2008BADB2B09), and the Project for Science and Technology Development of Jilin Province, PR of China (grant no. 20070210).

³ö°æÉÌ: ELSEVIER SCIENCE BV, PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS  
Web of Science ·ÖÀà: Medical Laboratory Technology  
ѧ¿ÆÀà±ð: Medical Laboratory Technology  
IDS ºÅ: 638PK  
ISSN: 0009-8981  
¼ìË÷ºÅ£ºUT  WOS:000280906800014
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5Â¥2011-10-31 10:03:23
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shy2400

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4Â¥: Originally posted by lccj158 at 2011-10-31 06:23:29:
лл°ïÖú¡£µÚһƪºÍµÚ¶þƪ¶¼ÊDZ»SCIÊÕ¼°¡£¬Âé·³ÔÙ²éһϰ¡£¬Ð»Ð»

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Detection of nerve growth factor (NGF) and its specific receptor (TrkA) in ejaculated bovine sperm, and the effects of NGF on sperm function  
×÷Õß: Li, C (Li, C.)1,2; Sun, Y (Sun, Y.)1,2,3; Yi, K (Yi, K.)4; Ma, Y (Ma, Y.)1,2; Sun, Y (Sun, Y.)1,2; Zhang, W (Zhang, W.)1,2; Zhou, X (Zhou, X.)1,2  
À´Ô´³ö°æÎï: THERIOGENOLOGY  ¾í: 74   ÆÚ: 9   Ò³: 1615-1622   DOI: 10.1016/j.theriogenology.2010.06.033   ³ö°æÄê: DEC 2010  
±»ÒýƵ´Î: 0 (À´×Ô Web of Science)  
ÒýÓõIJο¼ÎÄÏ×: 39 [ ²é¿´ Related Records ]     ÒýÖ¤¹ØÏµÍ¼      
ÕªÒª: The objective was to confirm the presence of nerve growth factor (NGF) and its specific receptor TrkA, in ejaculated bovine sperm, and to investigate the effects of NGF on specific aspects of bovine sperm function Both TrkA transcripts and immunoreactivity typical of the translated protein were detected by RT-PCR and western blotting However only the NGF protein was detected in bovine sperm using western blotting and there was no RT PCR evidence for NGF transcripts in sperm Using an immunofluorescent technique NGF immunoreactivity was localized to the sperm head and tail whereas that of TrkA was detected in the acrosomal cap nucleus, and tail regions When sperm were treated with exogenous NGF both leptin secretion and sperm viability were increased (P < 0 05) moreover the percentages of late apoptotic and dead sperm were increased (P < 0 05) However NGF had no effects on insulin secretion mitochondrial activity, intracellular calcium levels, or the acrosome reaction of sperm (P > 0 05) In conclusion the presence of TrkA transcript as well as NGF and TrkA immunoreactivity were confirmed in bovine sperm Furthermore exogenous NGF had significant effects on the secretion of leptin, cell viability and sperm apoptosis This study provided strong evidence that NGF/TrkA may have roles in regulation of sperm physiology and perhaps male fertility and infertility (C) 2010 Elsevier Inc All rights reserved  
ÎÄÏ×ÀàÐÍ: Article  
ÓïÖÖ: English  
×÷Õ߹ؼü´Ê: Bovine sperm; Nerve growth factor; Tyrosine kinase receptor; Expression  
KeyWords Plus: CALCIUM INFLUX; SPERMATOZOA; CELL; SECRETION; RNA; NEUROTROPHINS; EXPRESSION; VIABILITY; APOPTOSIS; SYSTEM  
ͨѶ×÷ÕßµØÖ·: Zhou, X (ͨѶ×÷Õß),Jilin Univ, Coll Anim Sci & Vet Med, 5333 Xi An Ave, Changchun 130062, Jilin Province, Peoples R China  
µØÖ·:
1. Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Jilin Province, Peoples R China
2. Jilin Univ, Jilin Prov Key Lab Anim Embryo Engn, Changchun 130062, Jilin Province, Peoples R China
3. Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Peoples R China
4. Hunan Inst Anim & Vet Sci, Changsha 410131, Hunan, Peoples R China  
»ù½ð×ÊÖúÖÂл:
»ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ
National High Technology Research and Development Program ("863" Program) of China  2008AA101003  
National Science and Technology Pillar Program of China  2008BADB2B09  
Project for Science and Technology Development of Jilin Province, P R of China  20070210  
Science and Technology Development Project of Changchun City  08GH08  

[ÏÔʾ»ù½ð×ÊÖúÐÅÏ¢][Òþ²Ø»ù½ð×ÊÖúÐÅÏ¢]   

This work was supported by the National High Technology Research and Development Program ("863" Program) of China (grant No 2008AA101003), The National Science and Technology Pillar Program of China (grant No 2008BADB2B09), The Project for Science and Technology Development of Jilin Province, P R of China (grant No 20070210), and Science and Technology Development Project of Changchun City (08GH08)

³ö°æÉÌ: ELSEVIER SCIENCE INC, 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA  
Web of Science ·ÖÀà: Reproductive Biology; Veterinary Sciences  
ѧ¿ÆÀà±ð: Reproductive Biology; Veterinary Sciences  
IDS ºÅ: 683UB  
ISSN: 0093-691X  
¼ìË÷ºÅ£ºWOS:000284499600012

µÚËÄÆª£º¿âÀïҲûÓУ¬ÄãÈ·¶¨ÊÇSCI¼ìË÷µÄÂð£¿
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Detection of the SRY Transcript and Protein in Bovine Ejaculated Spermatozoa  
×÷Õß: Li, CJ (Li, Chunjin)1,2; Sun, YF (Sun, Yongfeng)1,2,4; Yi, KL (Yi, Kangle)3; Li, CJ (Li, Chengjiao)1,2; Zhu, XL (Zhu, Xiaoling)1,2; Chen, L (Chen, Lu)1,2; Zhou, X (Zhou, Xu)1,2  
À´Ô´³ö°æÎï: ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES  ¾í: 24   ÆÚ: 10   Ò³: 1358-1364   DOI: 10.5713/ajas.2011.11062   ³ö°æÄê: OCT 2011  
±»ÒýƵ´Î: 0 (À´×Ô Web of Science)  
ÒýÓõIJο¼ÎÄÏ×: 27 [ ²é¿´ Related Records ]     ÒýÖ¤¹ØÏµÍ¼      
ÕªÒª: The sex-determining region on the Y (SRY) gene is important in mammalian sex determination and differentiation. We report a study of the abundance of SRY gene products in bovine ejaculated. RT-PCR experiments using RNA extracted from bovine spermatozoa with SRY-specific primers yielded a 456 bp product, but the amount of SRY mRNA in sperm was lower than that in the testes (p<0.01). A protein of approximately 27 KDa was detected by western blotting. The SRY transcript was detected in the midpiece of approximately half the spermatozoa by in situ hybridization, and the SRY protein was detected in the heads of half the spermatozoa by immunofluorescence, indicating that SRY mRNA and protein may only be present in Y-bearing spermatozoa. These results suggest that the SRY transcript and protein are present in bovine ejaculated Y-sperm. The roles of the SRY gene in spermatogenesis, sperm motility, and the sperm-oocyte interaction merit further investigation.  
ÎÄÏ×ÀàÐÍ: Article  
ÓïÖÖ: English  
×÷Õ߹ؼü´Ê: SRY Gene; Bovine Spermatozoa; In situ Hybridization; Bovine  
KeyWords Plus: MESSENGER-RNA; MATURE SPERMATOZOA; SEX DETERMINATION; EXPRESSION; SECRETION; RECEPTOR; SPERM; MOUSE  
ͨѶ×÷ÕßµØÖ·: Zhou, X (ͨѶ×÷Õß),Jilin Univ, Coll Anim Sci & Vet Med, 5333 Xian Ave, Changchun 130062, Jilin Province, Peoples R China  
µØÖ·:
1. Jilin Univ, Coll Anim Sci & Vet Med, Changchun 130062, Jilin Province, Peoples R China
2. Jilin Univ, Jilin Prov Key Lab Anim Embryo Engn, Changchun 130062, Jilin Province, Peoples R China
3. Hunan Inst Anim & Vet Sci, Changsha 410131, Hunan, Peoples R China
4. Jilin Agr Univ, Coll Anim Sci & Technol, Changchun 130118, Peoples R China  
µç×ÓÓʼþµØÖ·: xzhou65@vip.sina.com  
»ù½ð×ÊÖúÖÂл:
»ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ
National High Technology Research and Development Program ("863" Program) of China  2008AA101003  
National Science & Technology Pillar Program of China  2008BADB2B09  
Project for Science and Technology Development of Jilin Province, P.R. of China  20070210  
Science and Technology Development Project of Changchun City  08GH08  

[ÏÔʾ»ù½ð×ÊÖúÐÅÏ¢][Òþ²Ø»ù½ð×ÊÖúÐÅÏ¢]   

This work was supported by the National High Technology Research and Development Program ("863" Program) of China (No.2008AA101003), the National Science & Technology Pillar Program of China (No.2008BADB2B09), and the Project for Science and Technology Development of Jilin Province, P.R. of China (No. 20070210). Science and Technology Development Project of Changchun City (08GH08).

³ö°æÉÌ: ASIAN-AUSTRALASIAN ASSOC ANIMAL PRODUCTION SOC, ROOM 708 SAMMO SPOREX, 1638-32, SEOWON-DONG, GWANAK-GU, SEOUL 151-730, SOUTH KOREA  
Web of Science ·ÖÀà: Agriculture, Dairy & Animal Science  
ѧ¿ÆÀà±ð: Agriculture  
IDS ºÅ: 820BC  
ISSN: 1011-2367  
¼ìË÷ºÅ£ºWOS:000294879500005
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6Â¥2011-10-31 10:15:55
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