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南方科技大学公共卫生及应急管理学院2025级博士研究生招生报考通知
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373668567

木虫 (著名写手)


[求助] 求一段英译汉,在线翻译器的请绕道哦

First, 293 cells stably expressing Moloney gag and pol under control of cytomegalovirus (CMV) promoter-enhancer were transiently transfected by CaPO4 precipitation with 20 ug of pLXSN retroviral vector and 5 ug of the plasmid pMDG containing the vesicular stomatitis virus (VSVg) envelope downstream of the CMV promoter-enhancer.
and a 100 ul sample was loaded into disposable chambers and centrifuged for 1 min onto glass slides in a cytocentrifuge。Staining was verified by visualization under a light microscope, and the slides were mounted with glass cover slips with the use of Permount mounting media 。The population of cells was categorized as
either viable, apoptotic, or necrotic when analyzed by staining morphology. Counting was performed blind, meaning that each slide was given a number and the identity of each sample was not known during counting. The counting procedure made use of a light microscope at a magnification that allowed the incorporation of about 100 cells per field. Five fields were counted per slide, and each experimental condition was performed two times.
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fjtony163

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米米

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如果你还要这个翻译的话,我接了
6楼2011-10-11 14:22:43
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xiaokaizi

金虫 (著名写手)

5、请酌情设置悬赏金币,如:每50词(英文原文)不低于20金币;每50词(德文原文)不低于30金币;
2楼2011-10-04 11:04:41
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zhiqiancsu

金虫 (正式写手)

★ ★ ★ ★ ★
373668567(金币+1, 翻译EPI+1): 不带这么玩的哈,在线翻译! 2011-10-06 23:18:22
sltmac(金币-5, 翻译EPI-1): 机器翻译,EPI撤销,并扣5BB以示警戒~~~ 2011-10-07 08:24:06
sltmac: 2011-10-07 08:24:20
首先,293细胞中稳定表达莫洛尼GAG和POL下控制巨细胞病毒(CMV)启动子增强瞬时转染CaPO4降水20微克的逆转录病毒载体PLXSN和5质粒含有水泡性口炎病毒(VSVg)PMDG UG信封下游CMV启动子增强。
销售100微升样品装入一次性商会cytocentrifuge在1分钟到玻片离心染色光镜下可视化验证,并安装与使用Permount安装媒体的玻璃盖玻片的幻灯片。。细胞的人口被归类为
无论是可行的,细胞凋亡或坏死时,染色形态分析。点票是盲目的,意味着每张幻灯片被赋予了每个样本的数量和身份,没有在计数。点票程序在使用光学显微镜的放大,使约100个​​细胞每场纳入。五个领域分别计数每张幻灯片,每个实验条件下进行两次。
3楼2011-10-06 18:41:10
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agyz_0970

木虫 (小有名气)

【答案】应助回帖

8814402: 你试试看啊 2011-10-13 20:00:53
很难翻译吗,耍人呢
4楼2011-10-06 19:23:23
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