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[求助]
求一段英译汉，在线翻译器的请绕道哦
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First, 293 cells stably expressing Moloney gag and pol under control of cytomegalovirus (CMV) promoter-enhancer were transiently transfected by CaPO4 precipitation with 20 ug of pLXSN retroviral vector and 5 ug of the plasmid pMDG containing the vesicular stomatitis virus (VSVg) envelope downstream of the CMV promoter-enhancer. and a 100 ul sample was loaded into disposable chambers and centrifuged for 1 min onto glass slides in a cytocentrifuge。Staining was verified by visualization under a light microscope, and the slides were mounted with glass cover slips with the use of Permount mounting media 。The population of cells was categorized as either viable, apoptotic, or necrotic when analyzed by staining morphology. Counting was performed blind, meaning that each slide was given a number and the identity of each sample was not known during counting. The counting procedure made use of a light microscope at a magnification that allowed the incorporation of about 100 cells per field. Five fields were counted per slide, and each experimental condition was performed two times. |
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