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First, 293 cells stably expressing Moloney gag and pol under control of cytomegalovirus (CMV) promoter-enhancer were transiently transfected by CaPO4 precipitation with 20 ug of pLXSN retroviral vector and 5 ug of the plasmid pMDG containing the vesicular stomatitis virus (VSVg) envelope downstream of the CMV promoter-enhancer.
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