24小时热门版块排行榜    

查看: 311  |  回复: 0

seaseeboy

银虫 (小有名气)

[求助] 腺嘌呤美国质量标准翻译求助,请各位大侠帮帮忙

Organic impurities— (usp)
1、pH 7.0 Phosphate buffer— Dissolve 4.54 g of monobasic potassium phosphate in water to make 500 mL of solution. Dissolve 4.73 g of anhydrous dibasic sodium phosphate in water to make 500 mL of solution. Mix 38.9 mL of the monobasic potassium phosphate solution with 61.1 mL of the dibasic sodium phosphate solution. Adjust, if necessary, by the dropwise addition of the dibasic sodium phosphate solution to a pH of 7.0.
2、Standard preparations— Dissolve a suitable quantity of USP Adenine RS, accurately weighed, in hot water, cool, and dilute quantitatively with water to obtain a solution having a known concentration of about 0.19 mg per mL. Pipet 5-mL portions into three 100-mL volumetric flasks, dilute with 0.10 N hydrochloric acid, 0.010 N sodium hydroxide, and pH 7.0 Phosphate buffer, respectively, to volume, and mix.
3、Test preparations— Proceed as directed for Standard preparations, using the specimen in place of the Reference Standard.
4、Procedure— Obtain the UV absorption spectra of the Test preparations and the Standard preparations concomitantly, in 1-cm cells, covering the range between 220 nm and 320 nm, using water as the blank. The respective absorptivities, calculated on the dried basis, at the wavelengths of maximum absorbance, for each pair of corresponding solutions do not differ by more than 2.0%.
    这是有机杂质的检测方法,第1、2、3段是试剂和对照液及供试品液的配制,比较容易看懂,但是第4段就看迷茫了,不知道其测定的原理是什么,以下为自己翻译的内容,不知道对否,请各位战友指导指导,解释一下其测定的原理:
有机杂质(usp):
1、Ph7.0缓冲液:取4.54g磷酸二氢钾于500ml水中溶解,配制磷酸二氢钾溶液,取4.73g无水磷酸氢二钠于500ml水中溶解,配制磷酸氢二钠溶液。取上述磷酸二氢钾溶液38.9ml和磷酸氢二钠溶液61.1ml混合,如果有必要,用磷酸氢二钠溶液将混合液ph调整为7.0.
2、对照溶液:取适量腺嘌呤对照品,精密称重,与热水中溶解,冷却,用水定容配制成含腺嘌呤0.19mg/ml的溶液,各量取5ml于三个100ml量瓶中,分别用0.10mol/l盐酸,0.010mol/l氢氧化钠,Ph7.0缓冲液稀释,定容。
3、供试品溶液:按照对照品溶液配制的方法配制供试品溶液。
4、检查:采用1cm比色皿,使用水作为空白溶剂,用UV法对以上对照品和供试品溶液共6份分别进行波长扫描,波长范围为220nm~320nm。以无水物计算,每对对应的溶液在最大吸收波长处的吸收率差异不得大于2.0%。

第4段真的不懂其测定的原理,帮帮忙解释一下啊(腺嘌呤美国usp标准可在药物在线查询)
回复此楼

» 猜你喜欢

» 本主题相关商家推荐: (我也要在这里推广)

已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖

智能机器人

Robot (super robot)

我们都爱小木虫

相关版块跳转 我要订阅楼主 seaseeboy 的主题更新
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 085601材料工程专硕求调剂 +4 慕寒mio 2026-03-16 4/200 2026-03-17 17:17 by ruiyingmiao
[考研] 328求调剂,英语六级551,有科研经历 +3 生物工程调剂 2026-03-16 6/300 2026-03-17 17:13 by 生物工程调剂
[考研] 290求调剂 +6 孔志浩 2026-03-12 11/550 2026-03-17 14:41 by 周舟舟77
[考研] 268求调剂 +7 一定有学上- 2026-03-14 8/400 2026-03-17 13:10 by zz820
[考研] [导师推荐]西南科技大学国防/材料导师推荐 +3 尖角小荷 2026-03-16 6/300 2026-03-16 23:21 by 尖角小荷
[基金申请] 国自科面上基金字体 +6 iwuli 2026-03-12 7/350 2026-03-16 21:18 by sculhf
[考研] 0703化学调剂 290分有科研经历,论文在投 +7 腻腻gk 2026-03-14 7/350 2026-03-16 10:12 by houyaoxu
[考研] 26考研一志愿中国石油大学(华东)305分求调剂 +3 嘉年新程 2026-03-15 3/150 2026-03-15 13:58 by 哈哈哈哈嘿嘿嘿
[考研] 0856专硕279求调剂 +5 加油加油!? 2026-03-15 5/250 2026-03-15 11:58 by 2020015
[考研] 331求调剂(0703有机化学 +5 ZY-05 2026-03-13 6/300 2026-03-14 10:51 by Jy?
[基金申请] 有必要更换申报口吗 20+3 fannyamoy 2026-03-11 3/150 2026-03-14 00:52 by zhanghaozhu
[考研] 337一志愿华南理工0805材料求调剂 +7 mysdl 2026-03-11 9/450 2026-03-13 22:43 by JourneyLucky
[考研] 290求调剂 +9 ADT 2026-03-11 9/450 2026-03-13 21:55 by JourneyLucky
[考研] 315求调剂 +9 小羊小羊_ 2026-03-11 10/500 2026-03-13 21:13 by SXNU李老师
[考研] 301求调剂 +6 Liyouyumairs 2026-03-11 6/300 2026-03-13 20:11 by JourneyLucky
[考研] 考研调剂 +4 芬达46 2026-03-12 4/200 2026-03-13 16:04 by ruiyingmiao
[考研] 一志愿211化学学硕310分求调剂 +8 努力奋斗112 2026-03-12 9/450 2026-03-13 15:41 by JourneyLucky
[考研] 274求调剂 +3 S.H1 2026-03-12 3/150 2026-03-13 15:15 by JourneyLucky
[考研] 化工学硕306求调剂 +9 42838695 2026-03-12 9/450 2026-03-13 10:16 by houyaoxu
[基金申请] 提交后的基金本子,已让学校撤回了,可否换口子提交 +3 dut_pfx 2026-03-10 3/150 2026-03-11 08:38 by kudofaye
信息提示
请填处理意见