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40-300 NIH units/mg protein
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| 我买了凝血酶，是固体粉末，查了网上的相关详细资料，上面有40-300 NIH units/mg protein ，不知道是什么意思?请高手指点，我是想用此蛋白质做bingding实验，得配已知浓度的蛋白质溶液。 |
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2楼2011-09-13 19:14:40
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Thromb Haemost. 1995 Sep;74(3):900-3. The International and "NIH" units for thrombin--how do they compare? Gaffney PJ, Edgell TA. Abstract The current International Standard (IS) for alpha-thrombin was established in 1991. It was related in unitage with the 1st IS for Thrombin established in 1975 and contains 100 IU of thrombin activity. The National Institute of Health (NIH) standard (Lot J) is in common use for the calibration of commercial thrombin reagents and this study reports on a comparison of the thrombin units defined by these 2 standards. This study has indicated that one "NIH" unit is equivalent to 1.1 to 1.3 IU of thrombin, depending on the influence of PEG in the assay. A unitage ratio figure of 1.15 was recommended following analysis of data obtained in the presence and absence of PEG in the comparative assays. This was confirmed by amidolytic assay data. http://www.ncbi.nlm.nih.gov/pubmed/8571318 |
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