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北京石油化工学院2026年研究生招生接收调剂公告
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桌子一笑而过

新虫 (正式写手)


[交流] 40-300 NIH units/mg protein

我买了凝血酶,是固体粉末,查了网上的相关详细资料,上面有40-300 NIH units/mg protein ,不知道是什么意思?请高手指点,我是想用此蛋白质做bingding实验,得配已知浓度的蛋白质溶液。
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qdwmq

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Thromb Haemost. 1995 Sep;74(3):900-3.
The International and "NIH" units for thrombin--how do they compare?
Gaffney PJ, Edgell TA.

Abstract

The current International Standard (IS) for alpha-thrombin was established in 1991. It was related in unitage with the 1st IS for Thrombin established in 1975 and contains 100 IU of thrombin activity. The National Institute of Health (NIH) standard (Lot J) is in common use for the calibration of commercial thrombin reagents and this study reports on a comparison of the thrombin units defined by these 2 standards. This study has indicated that one "NIH" unit is equivalent to 1.1 to 1.3 IU of thrombin, depending on the influence of PEG in the assay. A unitage ratio figure of 1.15 was recommended following analysis of data obtained in the presence and absence of PEG in the comparative assays. This was confirmed by amidolytic assay data.

http://www.ncbi.nlm.nih.gov/pubmed/8571318
3楼2011-09-13 19:18:25
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qdwmq

木虫 (正式写手)



桌子一笑而过(金币+1):谢谢参与
瑞士巴塞尔素高大药厂的血凝酶商品名立止血
一个克氏单位(ku)指在37℃的试管内,使标准人血浆在60±20秒内凝固的立止血数量。
1ku相当于0.04nih凝血酶单位;
1巴曲酶单位(bu)相当于0.17nin凝血酶单位;
1ku相当于0.3国际单位(iu)的凝血酶。
2楼2011-09-13 19:14:40
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桌子一笑而过

新虫 (正式写手)


引用回帖:
3楼: Originally posted by qdwmq at 2011-09-13 19:18:25:
Thromb Haemost. 1995 Sep;74(3):900-3.
The International and "NIH" units for thrombin--how do they compare?
Gaffney PJ, Edgell TA.

Abstract

The current International Standard (IS)  ...

“ one "NIH" unit is equivalent to 1.1 to 1.3 IU of thrombin”,这样的话,与2楼那个信息不符呢,到底哪个可靠啊?
4楼2011-09-13 19:51:40
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桌子一笑而过

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引用回帖:
2楼: Originally posted by qdwmq at 2011-09-13 19:14:40:
瑞士巴塞尔素高大药厂的血凝酶商品名立止血
一个克氏单位(ku)指在37℃的试管内,使标准人血浆在60±20秒内凝固的立止血数量。
1ku相当于0.04nih凝血酶单位;
1巴曲酶单位(bu)相当于0.17nin凝血酶单位;
1ku ...

由“1ku相当于0.04nih凝血酶单位;1ku相当于0.3国际单位(iu)的凝血酶” 可得1NIH相当于7.5iu,与您楼下那位的信息不一致呢。
5楼2011-09-13 19:54:31
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