7楼: Originally posted by labrat at 2011-09-02 17:16:16:
Better solution is to change column to SEC column, else can try this combination for hydrophobic protein:
Buffer A: water, 50% formic acid
Buffer B: isopropanol, 5% formic acid
Gradient: 0-100%  ...

2楼: Originally posted by simber at 2011-08-15 15:33:06:
虽然不知道，帮顶。感觉目标峰拖尾很长，是不是考虑换根色谱柱试试看。