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1949stone

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[资源] 分子生物学-The PCR Revolution

Contributors page vii
Foreword by Russell Higuchi xi
Preface xiii
I BASIC TECHNOLOGIES
1 Real-time polymerase chain reaction 3
Mickey Williams
2 Thermostable enzymes used in polymerase chain reaction 12
Sudip K. Rakshit
3 Inventing molecular beacons 19
Fred Russell Kramer, Salvatore A. E. Marras, and Sanjay Tyagi
4 Rapid polymerase chain reaction and melting analysis 48
Carl T. Wittwer, Randy P. Rasmussen, and Kirk M. Ririe
5 Polymerase chain reaction and fluorescence chemistries:
deoxyribonucleic acid incarnate 70
Ben Sowers
6 Analysis of microribonucleic acid expression by quantitative real-time
polymerase chain reaction 80
Vladimir Benes, Jens Stolte, David Ibberson, Mirco Castoldi,
and Martina Muckenthaler
7 Miniaturized polymerase chain reaction for quantitative
clinical diagnostics 88
Melissa Mariani, Lin Chen, and Philip J. Day
8 The road from qualitative to quantitative assay: What is next? 110
Michael W. Pfaffl
9 Taking control of the polymerase chain reaction 129
Tania Nolan, Tanya Novak, and Jim Huggett
II APPLICATIONS
10 Polymerase chain reaction–based methods for the detection of solid
tumor cancer cells for clinical diagnostic and prognostic assays 155
Susan A. Burchill
v
11 Polymerase chain reaction and infectious diseases 173
Jim Huggett
12 Polymerase chain reaction and respiratory viruses 189
Ian M. Mackay
13 Polymerase chain reaction and severe acute respiratory syndrome 212
Weijun Chen and Yang Huanming
14 The MMR vaccine, measles virus, and autism – A cautionary tale 229
Stephen A. Bustin
15 Noninvasive prenatal diagnosis using cell-free fetal nucleic acids
in maternal plasma 243
Y. M. Dennis Lo
16 Polymerase chain reaction–based analyses of nucleic acids from
archival material 254
Ulrich Lehmann
17 Microarrays and quantitative real-time reverse
transcriptase–polymerase chain reaction 262
Elisa Wurmbach
18 Polymerase chain reaction in the detection of genetic variation 276
Pui-Yan Kwok
19 Polymerase chain reaction: A blessing and a curse for ancient
deoxyribonucleic acid research 284
Michael Hofreiter and Holger Ro¨mpler
Index 301
Color Plates follow page 214

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