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Previous work by wilson and blitchington (58) demonstrated somewhat similar results £¬with 25 of 50 clones (50%)classified as clos . leptum subgroup ,34% as bacteroides group and 10% as Clos-coccoides group .The disparity in the clostridial representation of the different clon libraries most probably reflects either inter-individual variations or disparity of the protocols .However ,bifidobacteria were again absent from the clone libraty ,In addition ,Eubacterium rectale was not covered in the same sample (58). These data highlight the difficulty to approach full coverage of the complex microbiota and further demonstrate that a polyphasic approach is pertinent .However,such work has enabled identification of previously unknown components of the fecal microbiota ,and the sequence data can be used to develop new probing to accurately quantify such bacteria . Work carried out as of the European Union (EU) human gut microbiota project using PCR clon libraries demonstrated that microbial diversity increased with age (57).in addition,the percentage of OTUS corresponding to known molecular species was highest in infant and lowest in the elderly subiects . Thus ,not only was the microbial diversity greater in the elderly subjects ,but also 92% of OTUs were undescribed (potentially novel )species . |
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