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yingli09

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[资源] 生物传感器用于癌症生物标志物检测(ACS最新)

In this study, we report the development of a nanoparticle-enhanced biosensor by integrating both the nanoparticles and immunoassay sensing technologies into a phase interrogation surface plasmon resonance (SPR) system for detecting antigen at a concentration as low as the femtomolar range. Our work has demonstrated that the plasmonic field extension generated from the gold film to gold nanorod (GNR) has led to a drastic sensitivity enhancement. Antibody-functionalized sensing film, together with antibody-conjugated GNRs, was readily served as a plasmonic coupling partner that can be used as a powerful ultrasensitive sandwich immunoassay for cancer-related disease detection. Experimentally, it was found that the bioconjugated GNR labels enhance the tumor necrosis factor alpha (TNF-α) antigen signal with more than 40-fold increase compared to the traditional SPR biosensing technique. The underlying principle was analyzed by simulating the near-field coupling between the sensing film and the GNR. The results have shown that GNRs were readily served as promising amplification labels in SPR sensing technology.[ Last edited by taozhikai on 2011-7-15 at 21:04 ]
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taozhikai

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2楼2011-07-15 11:15:33
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yingli09

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引用回帖:
Originally posted by taozhikai at 2011-07-15 11:15:33:
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3楼2011-07-15 11:16:09
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5楼2011-09-09 23:20:52
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