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lstt09nk(金币+5): 鼓励哦~希望对楼主有所帮助~ 2011-04-16 19:04:43
lstt09nk(EPI+1): 有理有据 2011-04-16 21:46:21
xinshi(金币+4): 2011-04-18 18:13:51
这是GE公司SuperdexTM 75说明书的部分介绍,我把说明书作为附件传上来

Column performance control
Check the performance of the column using the following procedure:
Sample: 100 μl 0.5% acetone (5mg/ml)
Eluent: buffer solution or distilled H2O
Flow rate: 0.75 ml/min, room temperature
Detection: 280 nm
Column efficiency, expressed as the number of theoretical plates per meter, N/m, is
calculated using the following equation:
N/m = 5.54 × (vr / w½平方 × (1000/L)
where
N/m = number of theoretical plates/meter
vr = peak elution distance (m)
w½ = peak width at half peak height (m)
L = bed height (m)

上述层析柱长0.24m。

假如上样量体积大,则峰宽与半峰宽更接近,比值更接近1,则理论塔板数/m更小,柱效低。另外,楼主所用的体积如果依据这个说明书来看就太大了。PS:用丙酮测柱效而不是用蛋白质,二楼说的是分离蛋白质时上样量的问题。

[ Last edited by 冼亮淀粉酶 on 2011-4-16 at 19:57 ]
3楼2011-04-16 15:20:54
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