The results showed that, MLPKf2 had only one copy.And through rough semi-quantitative of RT-PCR to detect the expression of MLPKf2, it was found that MLPKf2 expressed abundantly only in its stigma, with no expression in other tissues. This was different from MLPKf1 reported previously as MLPKf1 was distributed in the stigma, leaf and stem .
In this study, the self-incompatibility system of kale S13bS13b was used as test materials, the gene-specific primers was designed according to MLPKf2 gene fragments.
Using the RACE technology, from the library of RACE cDNA in kale Gene 5 'and 3' end partial sequence of clone were obtained with their length 1072bp and 737bp respectively.
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