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本帖产生 1 个翻译EPI ，点击这里进行查看

于凯

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本研究以羽衣甘蓝S13bS13b自交不亲和系为试验材料，根据MLPKf2基因的片段，设计基因特异性引物。利用RACE 技术，从羽衣甘蓝的RACE cDNA 文库中分别获得了基因5'和3'克隆端部分序列的长度为1072bp和737bp，然后经过全长拼接，通过设计全长引物进行PCR 得到MLPKf2的全长序列为1630 bp。利用四种酶酶切基因组DNA，进行Southern杂交检测。结果表明，MLPKf2只有一个拷贝。半定量RT-PCR粗略检测MLPKf2的表达水平发现，MLPKf2只在柱头中大量表达，在其他组织中几乎无表达。这不同于先前报道的MLPKf1，MLPKf1是在柱头、叶片和茎中均有分布。

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1楼 2011-04-09 20:40:36

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爱与雨下

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于凯(金币+50, 翻译EPI+1): 2011-04-09 21:30:25

In this study, the self-incompatibility system of kale S13bS13b was used as test materials, the gene-specific primers was designed according to MLPKf2 gene fragments.

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2楼2011-04-09 20:52:36

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爱与雨下

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Using the RACE technology, from the library of RACE cDNA in kale Gene 5 'and 3' end partial sequence of clone were obtained with their length 1072bp and 737bp respectively.

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3楼2011-04-09 20:57:31

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爱与雨下

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and then through(with) the stitching of full length and the design of primers, PCR was done to get the MLPKf2 Sequence with its full-length 1630 bp.

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4楼2011-04-09 21:02:12

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爱与雨下

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through Enzyme digestion of the genomic DNA by four enzymes , Southern blotting was detedted.

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5楼2011-04-09 21:05:38

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爱与雨下

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Originally posted by 爱与雨下 at 2011-04-09 21:05:38:
through Enzyme digestion of the genomic DNA by four enzymes , Southern blotting was detedted.

through Enzyme digestion of the genomic DNA by four enzymes , Southern blotting was detected.

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6楼2011-04-09 21:06:07

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爱与雨下

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The results showed that, MLPKf2 had only one copy.And through rough semi-quantitative of RT-PCR to detect the expression of MLPKf2, it was found that MLPKf2 expressed abundantly only in its stigma, with no expression in other tissues. This was different from MLPKf1 reported previously as MLPKf1 was distributed in the stigma, leaf and stem .

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7楼2011-04-09 21:11:14

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