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±¾Ñо¿ÒÔÓðÒ¸ÊÀ¶S13bS13b×Ô½»²»Ç׺ÍϵΪÊÔÑé²ÄÁÏ£¬¸ù¾ÝMLPKf2»ùÒòµÄƬ¶Î£¬Éè¼Æ»ùÒòÌØÒìÐÔÒýÎï¡£ÀûÓÃRACE ¼¼Êõ£¬´ÓÓðÒ¸ÊÀ¶µÄRACE cDNA ÎÄ¿âÖзֱð»ñµÃÁË»ùÒò5'ºÍ3'¿Ë¡¶Ë²¿·ÖÐòÁеij¤¶ÈΪ1072bpºÍ737bp£¬È»ºó¾­¹ýÈ«³¤Æ´½Ó£¬Í¨¹ýÉè¼ÆÈ«³¤ÒýÎï½øÐÐPCR µÃµ½MLPKf2µÄÈ«³¤ÐòÁÐΪ1630 bp¡£ÀûÓÃËÄÖÖøøÇлùÒò×éDNA£¬½øÐÐSouthernÔÓ½»¼ì²â¡£½á¹û±íÃ÷£¬MLPKf2Ö»ÓÐÒ»¸ö¿½±´¡£°ë¶¨Á¿RT-PCR´ÖÂÔ¼ì²âMLPKf2µÄ±í´ïˮƽ·¢ÏÖ£¬MLPKf2Ö»ÔÚÖùÍ·ÖдóÁ¿±í´ï£¬ÔÚÆäËû×éÖ¯Öм¸ºõÎÞ±í´ï¡£ÕⲻͬÓÚÏÈǰ±¨µÀµÄMLPKf1£¬MLPKf1ÊÇÔÚÖùÍ·¡¢Ò¶Æ¬ºÍ¾¥ÖоùÓзֲ¼¡£

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ÓÚ¿­(½ð±Ò+50, ·­ÒëEPI+1): 2011-04-09 21:30:25
In this study,  the self-incompatibility system of kale S13bS13b was used as test materials, the gene-specific primers was designed according to MLPKf2 gene fragments.
2Â¥2011-04-09 20:52:36
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Using the RACE technology, from  the library of  RACE cDNA in kale   Gene 5 'and 3' end partial sequence of clone  were obtained with their length  1072bp and 737bp respectively.
3Â¥2011-04-09 20:57:31
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and then through(with) the stitching of full length and the design of primers, PCR was done to get   the MLPKf2 Sequence with its full-length 1630 bp.
4Â¥2011-04-09 21:02:12
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through Enzyme digestion of the genomic DNA by four enzymes ,  Southern blotting was detedted.
5Â¥2011-04-09 21:05:38
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ÒýÓûØÌû:
Originally posted by °®ÓëÓêÏ at 2011-04-09 21:05:38:
through Enzyme digestion of the genomic DNA by four enzymes ,  Southern blotting was detedted.

through Enzyme digestion of the genomic DNA by four enzymes ,  Southern blotting was detected.
6Â¥2011-04-09 21:06:07
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
The results showed that, MLPKf2 had only one copy.And through rough semi-quantitative of RT-PCR to detect the expression of MLPKf2, it was  found that MLPKf2  expressed abundantly only in its stigma, with no expression in other tissues. This was different from MLPKf1 reported previously as MLPKf1 was distributed in the stigma, leaf and stem .
7Â¥2011-04-09 21:11:14
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