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| 本研究以羽衣甘蓝S13bS13b自交不亲和系为试验材料,根据MLPKf2基因的片段,设计基因特异性引物。利用RACE 技术,从羽衣甘蓝的RACE cDNA 文库中分别获得了基因5'和3'克隆端部分序列的长度为1072bp和737bp,然后经过全长拼接,通过设计全长引物进行PCR 得到MLPKf2的全长序列为1630 bp。利用四种酶酶切基因组DNA,进行Southern杂交检测。结果表明,MLPKf2只有一个拷贝。半定量RT-PCR粗略检测MLPKf2的表达水平发现,MLPKf2只在柱头中大量表达,在其他组织中几乎无表达。这不同于先前报道的MLPKf1,MLPKf1是在柱头、叶片和茎中均有分布。 |
2楼2011-04-09 20:52:36
3楼2011-04-09 20:57:31
4楼2011-04-09 21:02:12
5楼2011-04-09 21:05:38
6楼2011-04-09 21:06:07
| The results showed that, MLPKf2 had only one copy.And through rough semi-quantitative of RT-PCR to detect the expression of MLPKf2, it was found that MLPKf2 expressed abundantly only in its stigma, with no expression in other tissues. This was different from MLPKf1 reported previously as MLPKf1 was distributed in the stigma, leaf and stem . |
7楼2011-04-09 21:11:14










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