24小时热门版块排行榜    

北京石油化工学院2026年研究生招生接收调剂公告
查看: 1075  |  回复: 6
本帖产生 1 个 翻译EPI ,点击这里进行查看

于凯

金虫 (小有名气)


[交流] 求助翻译-----中译英 (不要在线翻译)

本研究以羽衣甘蓝S13bS13b自交不亲和系为试验材料,根据MLPKf2基因的片段,设计基因特异性引物。利用RACE 技术,从羽衣甘蓝的RACE cDNA 文库中分别获得了基因5'和3'克隆端部分序列的长度为1072bp和737bp,然后经过全长拼接,通过设计全长引物进行PCR 得到MLPKf2的全长序列为1630 bp。利用四种酶酶切基因组DNA,进行Southern杂交检测。结果表明,MLPKf2只有一个拷贝。半定量RT-PCR粗略检测MLPKf2的表达水平发现,MLPKf2只在柱头中大量表达,在其他组织中几乎无表达。这不同于先前报道的MLPKf1,MLPKf1是在柱头、叶片和茎中均有分布。

» 猜你喜欢

» 抢金币啦!回帖就可以得到:

查看全部散金贴

已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
于凯(金币+50, 翻译EPI+1): 2011-04-09 21:30:25
In this study,  the self-incompatibility system of kale S13bS13b was used as test materials, the gene-specific primers was designed according to MLPKf2 gene fragments.
2楼2011-04-09 20:52:36
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
Using the RACE technology, from  the library of  RACE cDNA in kale   Gene 5 'and 3' end partial sequence of clone  were obtained with their length  1072bp and 737bp respectively.
3楼2011-04-09 20:57:31
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
and then through(with) the stitching of full length and the design of primers, PCR was done to get   the MLPKf2 Sequence with its full-length 1630 bp.
4楼2011-04-09 21:02:12
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
through Enzyme digestion of the genomic DNA by four enzymes ,  Southern blotting was detedted.
5楼2011-04-09 21:05:38
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
引用回帖:
Originally posted by 爱与雨下 at 2011-04-09 21:05:38:
through Enzyme digestion of the genomic DNA by four enzymes ,  Southern blotting was detedted.

through Enzyme digestion of the genomic DNA by four enzymes ,  Southern blotting was detected.
6楼2011-04-09 21:06:07
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
The results showed that, MLPKf2 had only one copy.And through rough semi-quantitative of RT-PCR to detect the expression of MLPKf2, it was  found that MLPKf2  expressed abundantly only in its stigma, with no expression in other tissues. This was different from MLPKf1 reported previously as MLPKf1 was distributed in the stigma, leaf and stem .
7楼2011-04-09 21:11:14
已阅   回复此楼   关注TA 给TA发消息 送TA红花 TA的回帖
相关版块跳转 我要订阅楼主 于凯 的主题更新
普通表情 高级回复 (可上传附件)
最具人气热帖推荐 [查看全部] 作者 回/看 最后发表
[考研] 化学357分,考研调剂 +11 .Starry. 2026-04-04 12/600 2026-04-06 06:28 by houyaoxu
[考研] 332求调剂 +16 小小孟... 2026-04-05 17/850 2026-04-06 00:52 by fmesaito
[考研] 302分求调剂 一志愿安徽大学085601 +8 zyx上岸! 2026-04-04 8/400 2026-04-05 23:08 by lbsjt
[考研] 生物与医药273求调剂 +5 荔题南墙 2026-04-05 5/250 2026-04-05 22:04 by imissbao
[考研] 322求调剂 +3 嗯哼哼恒 2026-04-05 3/150 2026-04-05 19:52 by nepu_uu
[考研] 调剂 +3 好好读书。 2026-04-02 3/150 2026-04-05 13:02 by arrow8852
[考研] 材料调剂 +12 一样YWY 2026-04-02 13/650 2026-04-04 20:49 by 蓝云思雨
[考研] [调剂信息]085408光电信息 求调剂 总分291分数一英一 +3 iz11az 2026-04-02 3/150 2026-04-04 19:09 by 蓝云思雨
[考研] 一志愿南昌大学324求调剂 +9 hanamiko 2026-03-30 9/450 2026-04-04 11:04 by 猪会飞
[考研] 材料科学与工程考研 +10 拯救皮特托先生 2026-04-02 10/500 2026-04-03 23:57 by userper
[考研] 305求调剂 +3 77Qi 2026-04-03 3/150 2026-04-03 23:01 by qzxyhcsy
[考研] 材料科学与工程339求调剂 +12 hyz0119 2026-03-31 13/650 2026-04-03 18:33 by ls刘帅
[考研] 372分材料与化工(085600)一志愿湖南大学求调剂 +3 蓝笺片 2026-04-03 4/200 2026-04-03 17:58 by Jimmyandyou
[基金申请] esi高被引论文是不是能对中标有所加分和帮助呢 +5 redcom 2026-04-01 6/300 2026-04-03 15:15 by Howard28
[考研] 273求调剂 +20 李芷新1 2026-03-31 20/1000 2026-04-03 09:58 by linyelide
[考研] 312求调剂 +6 小小墨123 2026-04-02 7/350 2026-04-03 07:32 by jsw79
[考研] 一志愿华南师范大学-22408计算机-292分-求华南师范大学调剂 +4 爱读书的小鳄鱼 2026-04-02 4/200 2026-04-02 18:35 by 求调剂zz
[考研] 农学考研求调剂 +3 dkdkxm 2026-04-01 3/150 2026-04-02 16:04 by wangjagri
[考研] 求调剂 +4 图鉴212 2026-03-30 5/250 2026-04-01 15:32 by 图鉴212
[考研] 一志愿 南京航空航天大学 ,080500材料科学与工程学硕 +10 @taotao 2026-03-31 11/550 2026-04-01 09:43 by xiayizhi
信息提示
请填处理意见