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xxwd银虫 (小有名气)
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【求助/交流】Unstable A260/A230 value in RNA extraction 已有2人参与
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HI, I am using QIAGEN RNaeasy Mini kit isolate RNA form rats kidney, sometimes the RNA isolated has a low A260/A230 value because of the high 230 absorbtion. (wavelength scanning with Nanodrop(Thermo Fisher) shows a absorption peak near 230nm). I am sure it is not because of my disruption or homogenization problem because using the same homogenate did RNA extraction, sometimes got a low A260/A230 while sometimes absolutely normal. What's more, if the A230 high, then the A260 will be low. I want to know why and what can I do to solve this problem? Thanks! Both Chinese and English responses are welcome. |
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小木虫(金币+0.5):给个红包，谢谢回帖交流
amisking(金币+1): 鼓励发帖交流。 2011-04-01 20:20:22
小木虫(金币+0.5):给个红包，谢谢回帖交流
amisking(金币+1): 鼓励发帖交流。 2011-04-01 20:20:22
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What's A230? So far as I know, we use A260/A280 value to judge the conc. of RNA. And , if you realy use A230 and is sure about that, A230 varying with A260 together shows there maybe some phase or structure changes. so your "sometimes" is detect at the same time(I mean duplicate) or at different time? If at different time, maybe your stock situation makes the RNA change its structure, e.g. parts of double strand unwinded to single strand or some. |
2楼2011-04-01 09:04:40
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3楼2011-04-01 10:39:27