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ahaoli_86

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[交流] 【求助/交流】FITC 已有4人参与

FITC 荧光必须要键合上么,可以直接包覆使用么?与罗丹明相比,FITC有何优缺点?
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xhjggl

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小木虫(金币+0.5):给个红包,谢谢回帖交流
dhd997(金币+2): 挺热心的,可别是广告啊。 2011-03-26 08:52:54
上海生工有代理的以下试剂盒卖

HOOK™ Dye Labeling Kit (5/6) TAMRA-SE (Rhodamine)
蛋白罗丹明标记试剂盒       
Description:
(5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl ester, mixed isomers) is based on tetramethylrhodamine, one of the most common fluorophores used in the labeling of peptides, proteins, nucleic acids and nucleotides.(5/6) TAMRA absorbs green visible light at 546nm and emits an orange-red visible light at a maximum emission of 575nm.The NHS ester group provides the simplest and most commonly used group for labeling proteins. The succinimidyl ester group reacts with primary amines in lysine side chains and N-terminal amines forming a stable, covalent amide bond.This kit utilizes Tube-O-REACTOR™, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands-on time and effort. The (5/6) TAMRA-SE labeling reagent is also available in our single use OneQuant™ format.
Features:
Dye preweighed and supplied in single use  vials (Also available separately);Suitable for most proteins;Utilizes  single Tube Reactor for minimum hands on time and no protein loss.application for Labeling of proteins, peptides and nucleic acids with a red fluorescent dye.;Suitable for antibody labeling.Store: -15~-20℃
编号        包装        价格        产地
786-058        5 Reactions        2,709        GBios

HOOK™ Dye Labeling Kit (FITC)
蛋白FITC荧光素标记试剂盒       
Description:
FITC (fluorescein isothiocyanate) is a commonly used fluorescent label for proteins, as it contains the groups required for conjugating to amino, sulfhydryl, imidazoyl, tyrosyl or carbonyl groups of proteins. FITC has a molecular weight of 389, and excitation and emission wavelengths of 494nm and 520nm, respectively, therefore emitting green visible light.This kit utilizes Tube-O-Reactor™, which allows for the reactions to be performed in a single tube, with no loss of essential reagents and minimum hands-on time and effort.
Features:
Dye preweighed and supplied in single use  vials (Also available separately);Suitable for most proteins;Utilizes single Tube Reactor for minimum hands on time.application for Labeling of a green fluorescent dye to proteins and peptides. And Suitable for antibody labeling. Store: -15~-20℃
编号        包装        价格        产地
786-059           5 Reactions        2,709        GBios
2楼2011-03-25 21:58:38
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ahaoli_86

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引用回帖:
Originally posted by xhjggl at 2011-03-25 21:58:38:
上海生工有代理的以下试剂盒卖

HOOK™ Dye Labeling Kit (5/6) TAMRA-SE (Rhodamine)
蛋白罗丹明标记试剂盒       
Description:
(5/6) TAMRA-SE (5-(and-6)-Carboxytetramethylrhodamine succinimidyl este ...

你怎么做广告呢
3楼2011-03-26 10:54:24
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zdy1398

银虫 (正式写手)

区别 FITC贵
4楼2011-04-29 22:29:13
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ahaoli_86

金虫 (正式写手)

引用回帖:
Originally posted by zdy1398 at 2011-04-29 22:29:13:
区别 FITC贵

哦,这算一个,呵呵
5楼2011-04-30 16:34:26
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yuguanggui

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小木虫(金币+0.5):给个红包,谢谢回帖
西瓜(金币+4): 鼓励! 2011-05-04 16:30:10
FITC分子量较小,一般都是通过化学键偶联的。
可以根据试验的需要调节偶联比率
yu
6楼2011-05-04 15:02:25
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ahaoli_86

金虫 (正式写手)

引用回帖:
Originally posted by yuguanggui at 2011-05-04 15:02:25:
FITC分子量较小,一般都是通过化学键偶联的。
可以根据试验的需要调节偶联比率

谢谢楼上提供的信息,不知LS是否用过罗丹明?
7楼2011-05-05 09:04:40
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