| 查看: 1667 | 回复: 13 | |||
| 本帖产生 1 个 翻译EPI ，点击这里进行查看 | |||
[交流]
翻译通顺点
|
|||
| Extracts for ACC synthase (ACS) activity assays were prepared according to the method of Boller et al. (1979), and determined by the method of Lizada and Yang (1979) with slight modifications. One g tomato pericarp tissue was homogenized using a cold pestle and mortar in 5mL of extraction buffer which consisting of 4 mM dithiothreitol, 10mM pyridoxal phosphate and 1.0mM EDTA in 100mM pH 8.0 Hepes buffer. After centrifugation of the homogenate at 10,000×g for 20min, the supernatant was used for enzyme assays. All steps in the enzyme extraction were performed at 4◦C. ACC synthase activity was assayed in a 0.8mL reaction mixture containing 0.25mM S-adenosylmethionine, 50mM Hepes buffer and 10mM pyridoxal phosphate with 0.2mL of enzyme extract in test tubes. After incubation of the reaction mixture at 30 ◦C for 1 h, the tubes were sealed with a stopper and rubber septum, 0.1mL HgCl2 (80mM) and 0.1mL 5% NaOH–NaClO solution (1:2, v/v) were injected into the tubes, shaken and incubated for 2min. One mL of the gaseous portion was removed and assayed for ethylene as described above. ACS activity was expressed as nmol g−1 FWh−1. |
回复此楼» 猜你喜欢
本人女孩
已经有10人回复
-
二苯甲酮酸类衍生物
已经有4人回复
-
一志愿中科大材料与化工，353分还有调剂学校吗
已经有11人回复
-
294求调剂
已经有6人回复
-
335求调剂
已经有23人回复
-
279求调剂
已经有13人回复
-
304求调剂
已经有6人回复
-
322求调剂
已经有3人回复
-
271求调剂
已经有39人回复
-
收到复试调剂但是去不了
已经有6人回复
» 抢金币啦！回帖就可以得到:
-
辽石化新材料学院材料专业调剂 理转工 / 学专互调
+1/477
-
青岛大学/康复大学导师课题组2026年博士研究生
+3/359
-
复旦大学 课题组 2026年9月批入学-博士招聘
+1/85
-
长江大学化工学院环境学硕、专硕最后一批调剂（理学、工学均可）
+1/82
-
硕士招生
+1/40
-
fluent 化学反应部分预混、三维全尺寸计算慢与压力小问题请教
+1/38
-
招收08开头的学硕专硕均可以及0703，线上复试
+1/38
-
江西科技师范大学086生物与医药
+1/38
-
江西科技师范大学086生物与医药
+1/37
-
西安交通大学陕西省科技创新团队常年招聘保送生和考研生，每年2-4个名额
+1/35
-
寻南京大学BME海外优青
+1/34
-
武汉工程大学环境生态与生物工程学院资源生物技术团队师资博士后招聘启事
+1/16
-
大连海洋大学资源与环境专硕（0857）接收调剂学生
+1/15
-
求助请教各位:如果您的算法被顶尖院校学术不端怎么办？
+1/12
-
稀土发光分析｜核壳结构｜寿命拟合
+1/12
-
重庆三峡科技大学电磁场与微波技术专业接收调剂，考数二可调，系统4月13日开放
+1/8
-
南京林业大学-国家级青年人才团队 诚聘青年教授、博士后等青年才俊
+1/8
-
西安工程大学环境与化学工程学院研究生调剂（理学，农学可调，专硕可调学硕）
+1/4
-
鲁东大学水产学院杨建敏教授课题组，2026年水产学硕接收调剂3-4人
+1/2
-
郑州大学物理学院倪佩楠教授课题组诚邀博士后、青年教师及副教授加盟
+1/2
昔年残梦(金币+10, 翻译EPI+1): 2011-03-20 22:44:44
|
Extracts for ACC synthase (ACS) activity assays were prepared according to the method of Boller et al. (1979), and determined by the method of Lizada and Yang (1979) with slight modifications. One g tomato pericarp tissue was homogenized using a cold pestle and mortar in 5mL of extraction buffer which consisting of 4 mM dithiothreitol, 10mM pyridoxal phosphate and 1.0mM EDTA in 100mM pH 8.0 Hepes buffer 根据1979年Boller 等进行的ACC合成酶的提取物（ACS）的活性测定并经Lizada和杨（1979）修改过后的准备方法如下：将1g番茄果皮组织通过一个冷的杵和研钵进行研磨，直到将其研磨成匀浆。在研磨过程中加入5毫升的提取缓冲液，其中包含有4毫摩二硫苏糖醇，10毫摩磷酸吡哆醛，加有1毫摩EDTA的100毫摩、PH为8的HEPES缓冲液。 |
2楼2011-03-20 22:20:14
3楼2011-03-20 22:20:33
|
After centrifugation of the homogenate at 10,000×g for 20min, the supernatant was used for enzyme assays. All steps in the enzyme extraction were performed at 4◦C. ACC synthase activity was assayed in a 0.8mL reaction mixture containing 0.25mM S-adenosylmethionine, 50mM Hepes buffer and 10mM pyridoxal phosphate with 0.2mL of enzyme extract in test tubes. 将上述匀浆在10000转20分钟离心后，取上清液用于酶检测。整个酶提取过程都应该在4度环境下进行。 ACC合成酶的活性需要放入装有0.8mL的反应复合物的试管中进行测定，此复合物含0.25毫摩S -腺苷甲硫氨酸，50毫摩HEPES缓冲液和10毫摩带有0.2ml酶提取物的磷酸吡哆醛。 |
5楼2011-03-20 22:27:26
6楼2011-03-20 22:28:10
没办法啊，老师要让翻译，自己不会，只能求助别人了，唉，太难了，7楼2011-03-20 22:30:06
8楼2011-03-20 22:33:46
9楼2011-03-20 22:36:37
10楼2011-03-20 22:37:02
|
After incubation of the reaction mixture at 30 ◦C for 1 h, the tubes were sealed with a stopper and rubber septum, 0.1mL HgCl2 (80mM) and 0.1mL 5% NaOH–NaClO solution (1:2, v/v) were injected into the tubes, shaken and incubated for 2min. One mL of the gaseous portion was removed and assayed for ethylene as described above. ACS activity was expressed as nmol g−1 FWh−1. 此反应混合物在30度下温育1小时后，用橡胶隔膜和塞子将试管密封。将0.1毫升氯化汞（80毫摩）和0.1毫升5％氢氧化钠-次氯酸钠溶液（1:2，v / V选择）注入到这些试管中，震荡培养2分钟。将其中的气体部分引出并进行乙烯检测。 ACS的活性表现为nmol g−1 FWh−1。 不好意思，最后那个我查了，还是没弄明白神马意思。。。 |
11楼2011-03-20 22:42:02
12楼2011-03-20 22:42:46
13楼2011-03-20 22:45:53
14楼2011-03-20 22:54:00
简单回复
昔年残梦4楼
2011-03-20 22:27
回复
