| 查看: 1663 | 回复: 13 | |||
| 本帖产生 1 个 翻译EPI ，点击这里进行查看 | |||
[交流]
翻译通顺点
|
|||
| Extracts for ACC synthase (ACS) activity assays were prepared according to the method of Boller et al. (1979), and determined by the method of Lizada and Yang (1979) with slight modifications. One g tomato pericarp tissue was homogenized using a cold pestle and mortar in 5mL of extraction buffer which consisting of 4 mM dithiothreitol, 10mM pyridoxal phosphate and 1.0mM EDTA in 100mM pH 8.0 Hepes buffer. After centrifugation of the homogenate at 10,000×g for 20min, the supernatant was used for enzyme assays. All steps in the enzyme extraction were performed at 4◦C. ACC synthase activity was assayed in a 0.8mL reaction mixture containing 0.25mM S-adenosylmethionine, 50mM Hepes buffer and 10mM pyridoxal phosphate with 0.2mL of enzyme extract in test tubes. After incubation of the reaction mixture at 30 ◦C for 1 h, the tubes were sealed with a stopper and rubber septum, 0.1mL HgCl2 (80mM) and 0.1mL 5% NaOH–NaClO solution (1:2, v/v) were injected into the tubes, shaken and incubated for 2min. One mL of the gaseous portion was removed and assayed for ethylene as described above. ACS activity was expressed as nmol g−1 FWh−1. |
回复此楼» 猜你喜欢
291求调剂
已经有9人回复
-
一志愿中科大材料与化工，353分还有调剂学校吗
已经有9人回复
-
271求调剂
已经有37人回复
-
申博自荐
已经有3人回复
-
300求调剂
已经有10人回复
-
26年电池方向博士申请
已经有5人回复
-
急需调剂
已经有6人回复
-
RY：中国产出的科学垃圾论文，绝对数量和比例都世界第一
已经有18人回复
-
291求调剂
已经有8人回复
-
0854调剂
已经有9人回复
» 抢金币啦！回帖就可以得到:
-
科研需求-硫化镍钴精矿样品
+1/399
-
【坐标大连】92年本科男生，寻觅姻缘女生
+5/356
-
双一流南京医科大学招计算机、生物信息等方向研究生调剂
+1/136
-
重庆大学刘坤教授课题组招聘等离子体农业应用相关方向科研助理（农学、等离子体、电子
+1/84
-
广西科技大学化工专业现仍有较多调剂指标，欢迎符合条件的同学申请调剂
+1/41
-
西安交通大学陕西省科技创新团队常年招聘博士生，每年1-2个名额。
+1/36
-
昆明理工大学化学工程学院张恒、高欣老师课题组调剂招生，还有名额
+1/13
-
湖北文理学院物理与电子工程学院材料与化工专业研究生调剂
+1/11
-
稀土发光分析｜核壳结构｜寿命拟合
+1/10
-
湖南理工大学 化学、化工调剂指标充足，录取率高，欢迎第二批调剂的学生调剂
+1/9
-
中科院理化所热声热机团队招聘电气工程专业(电机方向)博士后/特别研究助理
+1/8
-
华南师范大学环境学院大分子体系多尺度模拟与实验研究团队招收2026年考研调剂生1名
+1/6
-
福建理工大学学硕专业代码08开头还有调剂名额
+1/5
-
最后调剂名额，丽水学院材料与能源工程学院
+1/5
-
26申博，ai+材料方向，求课题组推荐
+1/3
-
鲁东大学水产学院，水产学硕接收调剂3-4人
+1/3
-
西安工程大学环境与化学工程学院学硕调剂（专硕可调学硕）
+1/3
-
郑州大学物理学院倪佩楠教授课题组诚邀博士后、青年教师及副教授加盟
+1/3
-
武汉工程大学化学与环境工程学院招收调剂学生
+1/1
-
海南大学袁禹亮老师招收第二批博士，名额1个，2026年9月份入学（电解（海）水制氢）
+1/1
昔年残梦(金币+10, 翻译EPI+1): 2011-03-20 22:44:44
|
Extracts for ACC synthase (ACS) activity assays were prepared according to the method of Boller et al. (1979), and determined by the method of Lizada and Yang (1979) with slight modifications. One g tomato pericarp tissue was homogenized using a cold pestle and mortar in 5mL of extraction buffer which consisting of 4 mM dithiothreitol, 10mM pyridoxal phosphate and 1.0mM EDTA in 100mM pH 8.0 Hepes buffer 根据1979年Boller 等进行的ACC合成酶的提取物（ACS）的活性测定并经Lizada和杨（1979）修改过后的准备方法如下：将1g番茄果皮组织通过一个冷的杵和研钵进行研磨，直到将其研磨成匀浆。在研磨过程中加入5毫升的提取缓冲液，其中包含有4毫摩二硫苏糖醇，10毫摩磷酸吡哆醛，加有1毫摩EDTA的100毫摩、PH为8的HEPES缓冲液。 |
2楼2011-03-20 22:20:14
3楼2011-03-20 22:20:33
|
After centrifugation of the homogenate at 10,000×g for 20min, the supernatant was used for enzyme assays. All steps in the enzyme extraction were performed at 4◦C. ACC synthase activity was assayed in a 0.8mL reaction mixture containing 0.25mM S-adenosylmethionine, 50mM Hepes buffer and 10mM pyridoxal phosphate with 0.2mL of enzyme extract in test tubes. 将上述匀浆在10000转20分钟离心后，取上清液用于酶检测。整个酶提取过程都应该在4度环境下进行。 ACC合成酶的活性需要放入装有0.8mL的反应复合物的试管中进行测定，此复合物含0.25毫摩S -腺苷甲硫氨酸，50毫摩HEPES缓冲液和10毫摩带有0.2ml酶提取物的磷酸吡哆醛。 |
5楼2011-03-20 22:27:26
6楼2011-03-20 22:28:10
没办法啊，老师要让翻译，自己不会，只能求助别人了，唉，太难了，7楼2011-03-20 22:30:06
8楼2011-03-20 22:33:46
9楼2011-03-20 22:36:37
10楼2011-03-20 22:37:02
|
After incubation of the reaction mixture at 30 ◦C for 1 h, the tubes were sealed with a stopper and rubber septum, 0.1mL HgCl2 (80mM) and 0.1mL 5% NaOH–NaClO solution (1:2, v/v) were injected into the tubes, shaken and incubated for 2min. One mL of the gaseous portion was removed and assayed for ethylene as described above. ACS activity was expressed as nmol g−1 FWh−1. 此反应混合物在30度下温育1小时后，用橡胶隔膜和塞子将试管密封。将0.1毫升氯化汞（80毫摩）和0.1毫升5％氢氧化钠-次氯酸钠溶液（1:2，v / V选择）注入到这些试管中，震荡培养2分钟。将其中的气体部分引出并进行乙烯检测。 ACS的活性表现为nmol g−1 FWh−1。 不好意思，最后那个我查了，还是没弄明白神马意思。。。 |
11楼2011-03-20 22:42:02
12楼2011-03-20 22:42:46
13楼2011-03-20 22:45:53
14楼2011-03-20 22:54:00
简单回复
昔年残梦4楼
2011-03-20 22:27
回复
