| 查看: 1938 | 回复: 13 | |||
| 本帖产生 1 个 翻译EPI ，点击这里进行查看 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
[交流]
翻译通顺点
|
|||
| Extracts for ACC synthase (ACS) activity assays were prepared according to the method of Boller et al. (1979), and determined by the method of Lizada and Yang (1979) with slight modifications. One g tomato pericarp tissue was homogenized using a cold pestle and mortar in 5mL of extraction buffer which consisting of 4 mM dithiothreitol, 10mM pyridoxal phosphate and 1.0mM EDTA in 100mM pH 8.0 Hepes buffer. After centrifugation of the homogenate at 10,000×g for 20min, the supernatant was used for enzyme assays. All steps in the enzyme extraction were performed at 4◦C. ACC synthase activity was assayed in a 0.8mL reaction mixture containing 0.25mM S-adenosylmethionine, 50mM Hepes buffer and 10mM pyridoxal phosphate with 0.2mL of enzyme extract in test tubes. After incubation of the reaction mixture at 30 ◦C for 1 h, the tubes were sealed with a stopper and rubber septum, 0.1mL HgCl2 (80mM) and 0.1mL 5% NaOH–NaClO solution (1:2, v/v) were injected into the tubes, shaken and incubated for 2min. One mL of the gaseous portion was removed and assayed for ethylene as described above. ACS activity was expressed as nmol g−1 FWh−1. |
回复此楼» 猜你喜欢
科研人应该花精力去思考如何解决问题，而不是去凝练问题
已经有11人回复
-
要骂人了，新模版改版就是要淡化问题凝练这种虚的东西，结果有个评委还在说凝练得不够
已经有17人回复
-
基金系统什么内容也没有
已经有10人回复
-
学科评审组评审是指会评吗？
已经有5人回复
-
梦想
已经有8人回复
-
面上合作单位盖章
已经有9人回复
-
面上函评意见出来了，像什么等级？
已经有17人回复
-
为什么资助数各大高校都创新高，自己申请怎么就这么难
已经有14人回复
-
小白求助 投论文要求的highlights应该如何写
已经有4人回复
-
麻烦专家们看看评委们的意见（F口面上）
已经有12人回复
» 抢金币啦！回帖就可以得到:
-
云南大学丁军桥团队2027年博士招生
+1/810
-
【征稿中！】2026年机械、制造技术与材料国际会议 (MEMTM 2026)
+1/770
-
[求助]请问美国有哪些PC品牌厂商？
+1/189
-
哈尔滨工业大学（校本部）电信学院2027级博士招生
+1/181
-
2027级博士研究生——质谱分析
+5/130
-
广西大学作物学博士生招生
+2/50
-
香港城市大学生物物理/软物质材料课题组2026-2027年招博士生1名
+2/24
-
评职称需要，卖发明专利了:D有技术储备需求的设备厂商可联系
+1/19
-
山东大学-山东字节信息智能技术实验室2026年招聘博士后
+2/16
-
上海大学微电子学院杨军教授团队招收微电子/集成电路/材料/机械/微纳制造博士一名
+1/9
-
上海大学微电子学院杨军教授团队招收微电子/集成电路/材料/机械/微纳制造博士一名
+1/9
-
哈工大马樱招收2027级计算机类/集成电路类博士生
+1/9
-
论文投出去之前到底要不要先申请专利？被审稿周期坑过一次后的复盘
+1/5
-
【科研诚信】武汉轻工大学何江玲3篇论文：涉嫌抄袭、成组数据重复、制造虚假显著
+1/5
-
上海大学微电子学院杨军教授团队招收微电子/集成电路/材料/机械/微纳制造博士一名
+1/4
-
义翘神州推出全长p-Tau217蛋白，助力阿尔茨海默病下一代生物标志物检测开发
+1/3
-
国防补偿计划 推免硕士生及直博生
+1/2
-
2027硕士研究生推免-军事医学研究院生物技术药物代谢研究方向
+1/1
-
科研助理招聘
+1/1
-
结肠炎多维度分子解析：炎症‑免疫‑肠道屏障一体化检测策略
+1/1
|
After incubation of the reaction mixture at 30 ◦C for 1 h, the tubes were sealed with a stopper and rubber septum, 0.1mL HgCl2 (80mM) and 0.1mL 5% NaOH–NaClO solution (1:2, v/v) were injected into the tubes, shaken and incubated for 2min. One mL of the gaseous portion was removed and assayed for ethylene as described above. ACS activity was expressed as nmol g−1 FWh−1. 此反应混合物在30度下温育1小时后，用橡胶隔膜和塞子将试管密封。将0.1毫升氯化汞（80毫摩）和0.1毫升5％氢氧化钠-次氯酸钠溶液（1:2，v / V选择）注入到这些试管中，震荡培养2分钟。将其中的气体部分引出并进行乙烯检测。 ACS的活性表现为nmol g−1 FWh−1。 不好意思，最后那个我查了，还是没弄明白神马意思。。。 |
11楼2011-03-20 22:42:02
昔年残梦(金币+10, 翻译EPI+1): 2011-03-20 22:44:44
|
Extracts for ACC synthase (ACS) activity assays were prepared according to the method of Boller et al. (1979), and determined by the method of Lizada and Yang (1979) with slight modifications. One g tomato pericarp tissue was homogenized using a cold pestle and mortar in 5mL of extraction buffer which consisting of 4 mM dithiothreitol, 10mM pyridoxal phosphate and 1.0mM EDTA in 100mM pH 8.0 Hepes buffer 根据1979年Boller 等进行的ACC合成酶的提取物（ACS）的活性测定并经Lizada和杨（1979）修改过后的准备方法如下：将1g番茄果皮组织通过一个冷的杵和研钵进行研磨，直到将其研磨成匀浆。在研磨过程中加入5毫升的提取缓冲液，其中包含有4毫摩二硫苏糖醇，10毫摩磷酸吡哆醛，加有1毫摩EDTA的100毫摩、PH为8的HEPES缓冲液。 |
2楼2011-03-20 22:20:14
3楼2011-03-20 22:20:33
|
After centrifugation of the homogenate at 10,000×g for 20min, the supernatant was used for enzyme assays. All steps in the enzyme extraction were performed at 4◦C. ACC synthase activity was assayed in a 0.8mL reaction mixture containing 0.25mM S-adenosylmethionine, 50mM Hepes buffer and 10mM pyridoxal phosphate with 0.2mL of enzyme extract in test tubes. 将上述匀浆在10000转20分钟离心后，取上清液用于酶检测。整个酶提取过程都应该在4度环境下进行。 ACC合成酶的活性需要放入装有0.8mL的反应复合物的试管中进行测定，此复合物含0.25毫摩S -腺苷甲硫氨酸，50毫摩HEPES缓冲液和10毫摩带有0.2ml酶提取物的磷酸吡哆醛。 |
5楼2011-03-20 22:27:26
