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有一段英文翻译为中文
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| Both minnocycline and rifampin were deteced under the same separation conditions.The mobile phase cinsisted of KH2PO4(60%)and acetontrile(40%)with the final pH adjutsed to ~3.25 using 85% O-phosphoric acid.The HPL system was run under an isocratic reversed-phase condition with a flow rate of 1mL/min.Detection was done at a wavelength of 339 nm. Minocycine was eluted at 1.5 min,and rifamipin was detected at 13 min .In pilot trials,known amounts of antibiotics were added to 1-cm segments of uncoated catheters and extracted as outlined above.More than 90% of both antibiotics were recovered. After construction of standard curves,antibiotic cintrols were measured with a consistent accuracy of >85%. |
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2楼2011-03-08 11:21:46
hmilywq(金币+5): 2011-03-09 09:07:02
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在相同的分离条件下都检测到了二甲胺四环素和利福平。色谱流动相组成为60%的磷酸二氢钾和40%的乙腈，并用邻苯磺酸调节pH值在3.25左右。高效液相色谱在等度反相条件下运行，气流速度为1ml/min.检测波长为339nm。二甲胺四环素洗提1.5min,利福平洗提13min.实验过程中，向空导管中添加1cm已知量的抗生素，并在上述条件下分离。抗生素的回收量大于90%。 从绘制的标准曲线上可知，抗生素量检测的精确度大于85%。 ps：不是这个方向的，译得不好，敬请见谅。希望通过翻译，提高自己的理解水平，期待高手指点。 |
3楼2011-03-08 12:30:25
hmilywq(金币+7, 翻译EPI+1): 2011-03-09 09:06:45
hmilywq(金币+2): 2011-03-09 09:07:17
hmilywq(金币+2): 2011-03-09 09:07:17
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Both minocycline and rifampin were detected under the same separation conditions.在相同的分离条件下我们同时发现了米诺环素和利福平。 The mobile phase consists of KH2PO4(60%)and acetonitrile(40%)with the final pH adjutsed to ~3.25 using 85% O-phosphoric acid. 这个流动相由KH2PO4(60%)和通过85%的氧合磷酸稀释到最终PH为3.25的乙腈（40%）组成。 The HPLC system was run under an isocratic reversed-phase condition with a flow rate of 1mL/min.Detection was done at a wavelength of 339 nm. 这个高压液体色层法系统是用1mL/min的流速在等度反相状态的条件下运行，并在339nm的波长下进行检测。 Minocycline was eluted at 1.5 min,and rifampin was detected at 13 min. 其中美满霉素洗脱时间为1.5分钟，利福平检测时间为13分钟。 In pilot trials,known amounts of antibiotics were added to 1-cm segments of uncoated catheters and extracted as outlined above. 在试点试验中，将已知量得抗生素加入到涂层导管的1厘米处并如上文所述的方式提取出。 More than 90% of both antibiotics were recovered. After construction of standard curves,antibiotic cintrols were measured with a consistent accuracy of >85%. 其中超过90％的抗生素都能够被回收。在形成标准曲线后，测定抗生素的调控能力和已知的达到了85%以上的一致性。 嗯，我分开给你解释了一下。其中是有几个专业词的拼写不是太对，我给改了过来，你看一下，对一对是不是你所需要的。 |
4楼2011-03-08 12:44:54
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