Originally posted by wyyun3025 at 2011-03-07 20:09:12:
我正准备做免疫沉淀

Originally posted by 寒蕤 at 2011-03-07 17:24:34:
10cm皿的细胞不够吗？

Originally posted by jurkat.1640 at 2011-03-07 22:28:58:
我还没有做。但是《分子克隆》中的免疫共沉淀实验要30个长满细胞的10 cm皿。

Originally posted by 寒蕤 at 2011-03-08 15:57:47:
再请教一个问题：《分子克隆》上面的NETN中的氯化钠是100mM,怎么下面有说是“NETN,含900mM氯化钠”？这个是什么意思？谢谢！

Originally posted by wangleisdnu at 2011-03-08 21:11:53:
are you sure your target protein expressed at this stage?
If yes, try to use low concentration of wash buffer for wash steps
and also can try to use cross link
if you think their interaction is  ...

Originally posted by 寒蕤 at 2011-03-09 10:09:21:
谢谢，现在我用交联剂试试看。

Originally posted by wangleisdnu at 2011-03-09 15:27:16:
hopefully it will work
good luck

7楼: Originally posted by 寒蕤 at 2011-03-07 19:41:30
我用的裂解液是150mMNaCl,10mMTris-HCl pH8.0,1%NP40,1mM PMSF.
是不是去垢剂用CHAPS比NP40更好呢？这两个有什么区别？它们的浓度用多大比较合适呢？谢谢！...