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yanghong206

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使用乙醇沉淀法从海洋微生物发酵液中提取胞外多糖,再经732阳离子交换树脂分离得到了碱性多糖、弱酸性多糖和中酸性多糖这三种主要的多糖组分,并分别测定其还原力活性、DPPH自由基清除活性和羟基自由基清除活性等抗氧化活性指标,结果表明,弱酸性多糖具有最高的抗氧化活性。抑制真菌活性实验表明,这三种多糖组分对苹果轮纹病菌(Physalospora piricola)、瓜果腐霉病菌(Pythium aphanidermatum)、葡萄灰霉病菌(Botrytis cinerea)和甜瓜枯萎病菌(Fusarium pathogens)都表现出一定得抑菌活性,并且对葡萄灰霉病菌(Botrytis cinerea)的抑菌效果最为明显。

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ringzhu(金币+25): ~~给你讨回来的 2011-02-23 10:56:16
Extracted extracellular polysaccharide from fermentation liquid in sea water via ethanol precipitation,then obtained three main polysaccharides for instance alkalline polysaccharides, weak acid polysaccharides and acidic polysaccharides which were separated through 732 cation exchange resin and in succession determined their antioxidant activities like reduction activity, free radical scavenging activity and hydroxyl radical scavenging activity.The results indicated that weak acid polysaccharides had the highest antioxidant activity.The inhibitory-fungal antibiotic experiment showed that the three polysaccharides have some antimicrobial activity to Physalospora piricola, Pythium aphanidermatum,Botrytis cinerea and Fusarium pathogens,especially have high effect on Botrytis cinerea.
2楼2011-02-14 13:48:15
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yanghong206(金币+25, 翻译EPI+1): 2011-02-17 23:01:51
The ethanol precipitation method was adopted to extract exopolysaccharide from marine microbial fermentation. Then,  the extract was isolated against 732 cation exchange resin into three major polysaccharide components: alkaline polysaccharide, weak acidic polysaccharides and acidic polysaccharides. And the polysaccharides were assessed for antioxidant activity against reducing power activity, DPPH radical scavenging activity and hydroxyl radical scavenging activity assays.
3楼2011-02-14 15:17:25
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The results indicated that weak acid polysaccharides had the highest antioxidant activity. Following inhibitory-fungal antibiotic experiment showed that the three polysaccharides all have antimicrobial activity to Physalospora piricola, Pythium aphanidermatum,Botrytis cinerea and Fusarium pathogens to some extent, and  obvious effect to Botrytis cinerea especially.
4楼2011-02-14 15:25:07
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yanghong206(金币+25): 2011-02-17 23:02:01
ringzhu(金币-25): 与2楼相同 抄袭之嫌 敬请理解 有异议可举报 多谢~~ 2011-02-23 10:55:53
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