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gysongjia

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[交流] 求助翻译一段话

配制琼脂浓度为 2 %的营养型固体培养基 ,灭菌后待温度降至50 ℃左右时 ,在无菌条件下 ,将培养基倒入已灭菌并编号的培养皿( d ×h = 90 mm×15 mm)的皿盖中 ,其厚度为皿盖高度的 1/ 4左右为宜. 待培养基平板(为夹层的下层)冷却后 ,将富集液分别按 10 - 2、 10 - 3、 10 - 4稀释度吸取0. 2 mL 均匀涂布在平板上.静置 ,待涂布液迹基本渗入培养基后 ,倒入同种营养型固体培养基(为夹层的上层) ,其厚度要求略薄于下层(约2~3 mm) ;倒上层时 ,让液状培养基形成凸起状 ,随即迅速将培养皿的内皿(无菌)底朝下、口与皿盖同向地嵌入上层培养基;结果是内皿与培养基间无任何气泡 ,内皿周围有少量培养基逸进内外皿的侧壁间隙内 ,最终表现出二重皿法样的形式.

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LittleBush

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2楼2011-01-17 10:40:56
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LittleBush

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gysongjia(金币+30, 翻译EPI+1): 真不好意思啊 送你30金币 够意思把 2011-01-18 17:05:55
The following is the instruction of how to prepare the 2% agar nutrient culture medium。Pour the agar liquid to the pre-ordered and sterile petri dish covers with the height about 1/ 4 of the cover’s height when it cools down to about 50 ℃ When the plate(the bottom layer of the sandwich) totally cools down to the room temperature,evenly coat 0. 2 mL富集液(专有名词自己解决,以免误导)on the plate with 10 - 2、 10 - 3、 10 – 4 titrated concentrations respectively. When the 富集液 largely penetrate into the plate, pour the same type of culture medium(the top layer of the sandwich)  onto the plate in a convex shape with the thickness less than 2~3 mm of that of the plate. Then immediately cover the petri dish cover with the sterile  内皿 and then a tiny fraction of the liquid medium culture will overflow into the sidewalls of the内皿.Be careful not allow any air bubble form between the culture medium and the内皿 and all of the above procedures are carried out on sterile condictions.Finally you’’ get 二重皿法样 culture medium
3楼2011-01-17 11:23:47
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LittleBush

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楼主太不厚道了,
5楼2011-01-18 11:54:40
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2011-01-18 11:50   回复  
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