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| The following is the instruction of how to prepare the 2% agar nutrient culture medium¡£Pour the agar liquid to the pre-ordered and sterile petri dish covers with the height about 1/ 4 of the cover¡¯s height when it cools down to about 50 ¡æ When the plate(the bottom layer of the sandwich) totally cools down to the room temperature£¬evenly coat 0. 2 mL¸»¼¯Òº£¨×¨ÓÐÃû´Ê×Ô¼º½â¾ö£¬ÒÔÃâÎóµ¼£©on the plate with 10 - 2¡¢ 10 - 3¡¢ 10 ¨C 4 titrated concentrations respectively. When the ¸»¼¯Òº largely penetrate into the plate, pour the same type of culture medium(the top layer of the sandwich) onto the plate in a convex shape with the thickness less than 2¡«3 mm of that of the plate. Then immediately cover the petri dish cover with the sterile ÄÚÃó and then a tiny fraction of the liquid medium culture will overflow into the sidewalls of theÄÚÃó.Be careful not allow any air bubble form between the culture medium and theÄÚÃó and all of the above procedures are carried out on sterile condictions.Finally you¡¯¡¯ get ¶þÖØÃó·¨Ñù culture medium |
3Â¥2011-01-17 11:23:47
LittleBush
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5Â¥2011-01-18 11:54:40
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