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| 具体做法是将已经富集好的菌液采用无菌操作技术稀释成不同浓度。将含有质量分数为 2%琼脂的固体培养基熔化并保持在 50 ℃左右 ,在无菌条件下 ,向培养皿 (90 mm × 15 mm)的皿盖中倒入约 1 /3高度的固体培养基 ,待其刚刚冷凝后 ,将不同浓度的稀释液吸取适量 ,快速涂布平板上 ,使稀释液渗透约30 s后 ,在培养皿的中间位置倒入同种营养型固体培养基 ,直到将溢未溢的突起状态 ,随后迅速盖上皿盖并往下压 ,最终皿内不能有气泡。去掉培养皿内外两层侧壁间多余的琼脂,并在其中灌入适量熔化的石蜡 ,使培养皿侧壁缝隙被石蜡密封 ,尽量不要留有泡。 |
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gysongjia(金币+20, 翻译EPI+1): 谢谢 2011-01-16 18:57:14
ringzhu(金币+30): 辛苦~~ 2011-04-12 13:46:21
gysongjia(金币+20, 翻译EPI+1): 谢谢 2011-01-16 18:57:14
ringzhu(金币+30): 辛苦~~ 2011-04-12 13:46:21
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the bodily way of work：the concentrated microbial liquid was diluted to different concentration by aseptic technique.Solid medium which included mass fraction 2% of agar-agar and kept it under about 50 ℃.Under abacterial conditions,Solid medium was added into the lid of culture dish(90 mm × 15 mm), when came to 1 / 3 height of the lid ,please stopped.As soon as it condensed,we sucked up an appropriate amount of diluent of different concentration and coated on flat plate with the diluent quickly.When the time of diluent's penetration is 30s, the same nutritional kind of Solid medium was poured into the middle of culture dish.Until just will not overflow as protuberant state,we covered lid quickly and pressed down.Finally,there was no air bubble in the culture dish.We got rid of extra agar-agar between sidewall of outer layers and that of inner layers of the culture dish,then an appropriate amount of melting paraffin wax was infused into it,so that the crack on the sidewall of the culture dish was sealed off by paraffin wax and air bubble did not left as far as possible. |
2楼2011-01-16 15:52:21
