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gysongjia(金币+20, 翻译EPI+1): 谢谢 2011-01-16 18:57:14
ringzhu(金币+30): 辛苦~~ 2011-04-12 13:46:21
the bodily way of work：the concentrated microbial liquid was diluted to different concentration by aseptic technique.Solid medium which included mass fraction 2% of agar-agar and kept it under about 50 ℃.Under abacterial conditions,Solid medium was added into the lid of culture dish(90 mm × 15 mm),
when came to 1 / 3 height of the lid ,please stopped.As soon as it condensed,we sucked up an appropriate amount of diluent of different concentration and coated on flat plate with the diluent quickly.When the time of diluent's penetration is 30s, the same nutritional kind of Solid medium was poured into the middle of culture dish.Until just will not overflow as protuberant state,we covered lid quickly and pressed down.Finally,there was no air bubble in the culture dish.We got rid of extra agar-agar between sidewall of
outer layers and that of inner layers of the culture dish,then an appropriate amount of melting paraffin wax was infused into it,so that the crack on the sidewall of the culture dish was sealed off by paraffin wax and air bubble did not left as far as possible. |
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