²é¿´: 380  |  »Ø¸´: 3

hnndpt

Ìú³æ (ÕýʽдÊÖ)

[½»Á÷] ¡¾ÇóÖú/½»Á÷¡¿ÎÄÏ×ÖеÄÒ»µãÎÊÌ⣬Àí½â²»ÁË£¡£¡£¡

Library screening, cloning and sequencing
Two degenerate primers (TL3, 5¡é-TTYGAICAYCCITAYCCICCIACIAARYIIAGTTT-
3¡é and TL4, 5¡é-CATIGGGTCRATICCRTTIGGICCIGCIACIGTNGG-
3¡é) were designed from AtTTG1 to amplify 700 bp from putative cotton homologues.Amplified fragments were ligated to pGEM-T Easy (Promega) and used to transform E. coli DH5-a F0 cells (Promega). The insert from one clone was released by EcoRI digestion, oligolabelled (Hodgson and Fisk, 1987) and used to
probe a 12 DPA (days post-anthesis) cotton fibre cDNA library.

resultÖÐÓëÖ®ºôÓ¦µÄ½á¹û£º
Initially, two degenerate TTG1 PCR primers were used to amplify a 700 bp fragment from cotton genomi cDNA. Nucleotide sequencing of four clones
revealed two distinct, but very similar, sequences.

ÎÊÌ⣺×îºóÔõô»á³öÏÖ Nucleotide sequencing of four clones£¿Õâ¸öfourÊÇÔõôÀ´µÄ£¿ÓÃ1¶Ô¼ò²¢ÒýÎïÈ¥À©Ôö700bpµÄƬ¶Î£¬¼ÙÈç˵À©Ôö³öÁË4¸ö²»Í¬µÄƬ¶Î£¬Õâ4¸öƬ¶ÎÓÖÔõô·Ö¿ª£¿ÖصãÊÇÄǸö4 clonesÊÇÔõô»ØÊ£¿¿´ÁËÁ½ÆªÎÄÏ×¶¼ÓÐÕâ¸ö¡£
»Ø¸´´ËÂ¥
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

brightfuture01

ľ³æÖ®Íõ (ÎÄ̳¾«Ó¢)

ÎÒ°®´òÀÏ»¢

ÎÄÏ׽ܳö¹±Ï×

hnndpt(½ð±Ò+5):·Ç³£¸Ðл£¡ 2010-10-07 17:03:56
ºÇºÇ£¬²»ÊÇÌáµ½Á¬½Óµ½ pGEM-T EasyÔØÌåÉÏÁËô£¬È»ºóÓÖËæ¼´²âÐòÁË¡£»òÕß¾­¹ýRFLPʹ֮·ÖÐÍ£¬×îºóÓÐÁ½¸öÃ÷ÏÔ²îÒìµÄpattern£¬ËÍÈ¥²âÐò£¬µÃµ½two distinct, but very similar, sequences¡£ÔÙ×Ðϸ¿´¿´ÎÄÕ£¬Ó¦¸ÃÓÐrandomly select »òÕßRFLP·ÖÐÍÖ®ÀàµÄ´ÊÓïµÄ¡£
The world is a fine place and worth fighting for. I agree with the second part.
2Â¥2010-10-07 08:43:54
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

hnndpt

Ìú³æ (ÕýʽдÊÖ)

ÒýÓûØÌû:
Originally posted by brightfuture01 at 2010-10-07 08:43:54:
ºÇºÇ£¬²»ÊÇÌáµ½Á¬½Óµ½ pGEM-T EasyÔØÌåÉÏÁËô£¬È»ºóÓÖËæ¼´²âÐòÁË¡£»òÕß¾­¹ýRFLPʹ֮·ÖÐÍ£¬×îºóÓÐÁ½¸öÃ÷ÏÔ²îÒìµÄpattern£¬ËÍÈ¥²âÐò£¬µÃµ½two distinct, but very similar, sequences¡£ÔÙ×Ðϸ¿´¿´ÎÄÕ£¬Ó¦¸ÃÓÐrand ...

·Ç³£¸Ðл£¡»¹ÓÐÒ»µãÐèÒªÇë½Ì£º
ÎÄÖÐûÓÐÌáµ½RFLP£¬Èç¹ûÓüò²¢ÒýÎïÀ©Ôö³öÀ´µÄÐòÁÐÁ¬½Óµ½ÔØÌåÒÔºó£¬Ôõô¶ÔÆä²âÐò£¿Ëæ»úÑ¡Ôñ¼¸¸ö¿Ë¡½øÐвâÐòÂð£¿ÕâÑùµÄ»°»á²»»á°ÑÀ©Ôö³öµÄ²»Í¬ÐòÁÐÒÅ©ÁË£¿£¨Æ©Èç˵À©Ôö³öÁË5¸ö²»Í¬µÄƬ¶Î£¬¶øÄãÈ´Ñ¡ÔñÁË4¸ö¿Ë¡ËÍÈ¥²âÐò£¬ÕâÑùÊÇ·ñ¾ÍÒÅ©ÁËÒ»¸ö£¿£©
3Â¥2010-10-07 17:03:42
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

brightfuture01

ľ³æÖ®Íõ (ÎÄ̳¾«Ó¢)

ÎÒ°®´òÀÏ»¢

ÎÄÏ׽ܳö¹±Ï×

ÒýÓûØÌû:
Originally posted by hnndpt at 2010-10-07 17:03:42:

·Ç³£¸Ðл£¡»¹ÓÐÒ»µãÐèÒªÇë½Ì£º
ÎÄÖÐûÓÐÌáµ½RFLP£¬Èç¹ûÓüò²¢ÒýÎïÀ©Ôö³öÀ´µÄÐòÁÐÁ¬½Óµ½ÔØÌåÒÔºó£¬Ôõô¶ÔÆä²âÐò£¿Ëæ»úÑ¡Ôñ¼¸¸ö¿Ë¡½øÐвâÐòÂð£¿ÕâÑùµÄ»°»á²»»á°ÑÀ©Ôö³öµÄ²»Í¬ÐòÁÐÒÅ©ÁË£¿£¨Æ©Èç˵À©Ôö³öÁË5¸ö²» ...

ÊÇËæ»úÌô¿Ë¡²âÐòµÄ£¬ºÇºÇ¡£TÔØÌåÉϲ»ÊÇÓÐÒýÎïô£¬¾ÍÓÃTÔØÌåͨÓÃÒýÎï²âÐò¡£
»á³öÏÖÀàËÆÇé¿öµÄ£¬ºÇºÇ£¬ËùÒÔÒ»°ãÖÁÉÙ¶¼ËÍ10¶à¸öÑùÆ·È¥²âÐòµÄ¡£²»¹ý°´ÕÕÄãÕâÆªÎÄÕµÄÒâ˼ÊÇËæ»úÌôÁË4¸ö²âÐò£¬È»ºóµÃµ½Á½¸ö²»Í¬µÄÐòÁС£

ÎÒÔø¾­×ö¹ýÒ»¸öÀàËÆµÄ¹¤×÷£¬28¸ö¿Ë¡¾­¹ýRFLPºó·ÖΪÁË13¸öpattern£¬ËùÒÔÒªÊÇËÍÉÙÁ˵ϰ£¬¹À¼Æ¾Í²»»á³öÀ´Õâô¶àÁË¡£Ò»°ãÇé¿ö϶¼ÊÇ×öÒ»¸öRFLPµÄ£¬È»ºóËͲ»Í¬µÄPattern²âÐò¡£
The world is a fine place and worth fighting for. I agree with the second part.
4Â¥2010-10-07 19:01:14
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ hnndpt µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] Çóµ÷¼Á +7 Ê®Èý¼ÓÓÍ 2026-03-21 7/350 2026-03-23 23:48 by ÈÈÇéɳĮ
[¿¼ÑÐ] Ò»Ö¾Ô¸ÖÐÄÏ´óѧ»¯Ñ§Ñ§Ë¶0703×Ü·Ö337Çóµ÷¼Á +5 niko- 2026-03-22 5/250 2026-03-23 22:01 by fuyu_
[¿¼ÑÐ] ¹¤¿Æ0856Çóµ÷¼Á +5 ãåÎö͡͡ 2026-03-21 5/250 2026-03-23 17:56 by º£ÉªÞ±-
[¿¼ÑÐ] 291 Çóµ÷¼Á +4 »¯¹¤2026½ì±ÏÒµÉ 2026-03-21 5/250 2026-03-23 16:46 by »¯¹¤2026½ì±ÏÒµÉ
[¿¼ÑÐ] ÇóÀÏʦÊÕÎÒ +3 zzh16938784 2026-03-23 3/150 2026-03-23 12:56 by ztnimte
[¿¼ÑÐ] 070300£¬Ò»Ö¾Ô¸±±º½320Çóµ÷¼Á +3 Jerry0216 2026-03-22 5/250 2026-03-23 09:16 by ¡£¡£ÌÃÌÃ
[¿¼ÑÐ] 289²ÄÁÏÓ뻯¹¤£¨085600£©BÇøÇóµ÷¼Á +3 ÕâôÃû×ÖÕ¦Ñù 2026-03-22 4/200 2026-03-22 17:56 by ÔÆÃñ´óÀîÀÏʦ
[¿¼ÑÐ] Ò»Ö¾Ô¸ÖÐÄÏ»¯Ñ§£¨0703£©×Ü·Ö337Çóµ÷¼Á +9 niko- 2026-03-19 10/500 2026-03-22 16:08 by ColorlessPI
[¿¼ÑÐ] ÉúÎïѧµ÷¼Á +5 Surekei 2026-03-21 5/250 2026-03-22 14:39 by tcx007
[¿¼ÑÐ] Çóµ÷¼Á +5 Zhangbod 2026-03-21 7/350 2026-03-22 13:13 by Zhangbod
[¿¼ÑÐ] Çóµ÷¼Á +7 Auroracx 2026-03-22 7/350 2026-03-22 12:38 by ËØÑÕÇã³Ç1988
[¿¼ÑÐ] ¿¼Ñе÷¼Á +3 ºôºô£¿~+123456 2026-03-21 3/150 2026-03-21 20:04 by Î޼ʵIJÝÔ­
[¿¼ÑÐ] 311Çóµ÷¼Á +3 Ó¸ҵÄСÎâ 2026-03-20 3/150 2026-03-21 17:40 by ColorlessPI
[¿¼ÑÐ] ²ÄÁÏ 336 Çóµ÷¼Á +3 An@. 2026-03-18 4/200 2026-03-21 01:39 by JourneyLucky
[¿¼ÑÐ] 274Çóµ÷¼Á +10 S.H1 2026-03-18 10/500 2026-03-20 23:51 by JourneyLucky
[¿¼ÑÐ] 22408 344·Ö Çóµ÷¼Á Ò»Ö¾Ô¸ »ªµç¼ÆËã»ú¼¼Êõ +4 solanXXX 2026-03-20 4/200 2026-03-20 23:49 by alg094825
[¿¼ÑÐ] 321Çóµ÷¼Á +9 ºÎÈó²É123 2026-03-18 11/550 2026-03-20 23:19 by JourneyLucky
[¿¼ÑÐ] ²ÄÁÏѧ˶318Çóµ÷¼Á +5 February_Feb 2026-03-19 5/250 2026-03-19 23:51 by 23Postgrad
[¿¼ÑÐ] ²ÄÁÏ¿¼Ñе÷¼Á +3 xwt¡£ 2026-03-19 3/150 2026-03-19 11:22 by wãåÑôw
[¿¼ÑÐ] ±¾¿ÆÖ£ÖÝ´óѧÎïÀíѧԺ£¬Ò»Ö¾Ô¸»ª¿Æ070200ѧ˶£¬346Çóµ÷¼Á +4 ÎÒ²»ÊÇÒ»¸ù´Ð 2026-03-18 4/200 2026-03-19 09:11 by ¸¡ÔÆ166
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û