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wdyym

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yushan3758

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还可以保护目的dna
5楼2010-07-25 12:40:32
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wangleisdnu

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看天(金币+3):鼓励回答 谢谢 2010-07-21 09:04:41
the function of sperm dna in yeast transformation is to reduce membran binding to our plasmid. as we know, yeast cell is different with bacteria cell. in yeast, its membran can bind plasmid, so if we insert plasmid to yeast without addition of carrier dna, so we'll lost our plasmid in large number...
有勇气去改变你可以改变的,有度量去容忍你不能改变的,有智慧去区分上面的两种情况http://bbs.bioon.net/bbs/?66210
2楼2010-07-20 22:59:16
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wangleisdnu

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scelab(金币+5):high hand~ :tiger23: 2010-07-20 23:48:50
You can read these articles. they will help you about purposes of usage of all chemical and biological components.
we performed yeast transformation in order to knock-out a gene, and we applied protocols in these articles. So, it works.

1-) High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

R Daniel Gietz1 & Robert H Schiestl2

2-) Schiestl, R.H. & Gietz, R.D. High efficiency transformation of intact yeast cells using single-stranded nucleic acids as carrier. Curr. Genet. 16, 339â???346 (1989).

3-)Gietz, R.D. & Woods, R.A. Genetic transformation of yeast. BioTechniques 30, 816â???831 (2001).
有勇气去改变你可以改变的,有度量去容忍你不能改变的,有智慧去区分上面的两种情况http://bbs.bioon.net/bbs/?66210
3楼2010-07-20 23:05:55
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wdyym

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4楼2010-07-25 11:04:31
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