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wangleisdnu

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¿´Ìì(½ð±Ò+3):¹ÄÀø»Ø´ð лл 2010-07-21 09:04:41
the function of sperm dna in yeast transformation is to reduce membran binding to our plasmid. as we know, yeast cell is different with bacteria cell. in yeast, its membran can bind plasmid, so if we insert plasmid to yeast without addition of carrier dna, so we'll lost our plasmid in large number...
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2Â¥2010-07-20 22:59:16
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wangleisdnu

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scelab(½ð±Ò+5):high hand~ :tiger23: 2010-07-20 23:48:50
You can read these articles. they will help you about purposes of usage of all chemical and biological components.
we performed yeast transformation in order to knock-out a gene, and we applied protocols in these articles. So, it works.

1-) High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method

R Daniel Gietz1 & Robert H Schiestl2

2-) Schiestl, R.H. & Gietz, R.D. High efficiency transformation of intact yeast cells using single-stranded nucleic acids as carrier. Curr. Genet. 16, 339â???346 (1989).

3-)Gietz, R.D. & Woods, R.A. Genetic transformation of yeast. BioTechniques 30, 816â???831 (2001).
ÓÐÓÂÆøÈ¥¸Ä±äÄã¿ÉÒԸıäµÄ,ÓжÈÁ¿È¥ÈÝÈÌÄã²»ÄܸıäµÄ,ÓÐÖÇ»ÛÈ¥Çø·ÖÉÏÃæµÄÁ½ÖÖÇé¿öhttp://bbs.bioon.net/bbs/?66210
3Â¥2010-07-20 23:05:55
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wdyym

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4Â¥2010-07-25 11:04:31
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yushan3758

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5Â¥2010-07-25 12:40:32
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Trista`

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Сľ³æ: ½ð±Ò+0.5, ¸ø¸öºì°ü£¬Ð»Ð»»ØÌû
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646415Â¥: Originally posted by wangleisdnu at 2010-07-20 23:05:55
You can read these articles. they will help you about purposes of usage of all chemical and biological components.
we performed yeast transformation in order to knock-out a gene, and we applied prot ...

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