| 查看: 16985 | 回复: 44 | |||||||
| 【奖励】 本帖被评价30次，作者jingcp200320增加金币 23.25 个 | |||||||
[资源]
【分享】100多种培养基配方！
|
|||||||
|
1. Acetobacter Medium (醋酸菌培养基) Glucose (葡萄糖) 100g Yeasst extract (酵母膏) 10g CaCO3 20g Agar (琼脂) 15g Distilled water (蒸馏水) 1000ml Adjust (调) pH to 6.8 适用范围：恶臭醋酸杆菌混浊变种 2. Nutrient Agar (营养肉汁琼脂) Pepton (蛋白胨) 5g Beef extract (牛肉膏) 30g NaCl 5g Agar (琼脂) 15g Distilled water (蒸馏水) Adjust (调) pH to 7.0-7.2 [Note]：When cultivation of Bacillus，5mg of to MnSO4.H2O may be added . It is favorable to promote spore formation . 适用范围：产气气杆菌、粪产碱杆菌、蜡状芽孢杆菌、蜡状芽孢杆菌蕈状变种、地衣形芽孢杆菌、巨大芽孢杆菌、多粘芽孢杆菌、尘 埃芽孢杆菌、短小芽孢杆菌、嗜热脂肪芽孢杆菌、枯草芽孢杆菌、枯草芽孢杆菌深黑变种、苏云金芽孢杆菌、苏云金芽孢杆菌蜡螟亚 种(青虫菌)、苏云金芽孢杆菌戈尔斯德变种、苏云金芽孢杆菌猝倒亚种、产氨短杆菌、黄色短杆菌、谷氨酸棒状杆菌、北京棒杆菌、 大肠埃希氏菌(大肠杆菌)、铜绿假单胞菌(绿脓杆菌)、凸形假单胞杆菌、荧光假单胞菌、弯曲假单胞菌、恶臭假单胞菌、假单胞杆菌、 藤黄八叠球菌、亚黄八叠球菌、尿素八叠球菌、金黄色葡萄球菌、运动发酵单孢菌 3. Azotobacter Medium (固氮菌培养基) KH2PO4 0.2g K2HPO4 0.8g MgSO4.7H2O 0.2g CaSO4.2H2O 0.1ｇ Na2MoO4.2H2O Trace(微量) Yeast axtract(酵母膏) 0.5g Mannitol(甘露醇) 20g FeCl3 Tract(微量) Distilled water (蒸馏水) 1000ml Agar (琼脂) 15g Adjust (调) pH to 7.2 适用范围：固氮菌、胶质芽孢杆菌 4. Corn Meal Medium (玉米粉培养基) Maize flour (玉米粉) 5g Peptone (蛋白胨) 0.1g Glucose (葡萄糖) 1g Tap water (自来水) 1000ml [Note]:Boil the mixture in autoclave at 121℃ for 1 hr. distribute the medium into 18ⅹ18 mm tubes , each contains 10 ml of the li quid , then autoclave at 121℃ for 1 hr . again (15磅蒸煮1小时,分装入18ⅹ18毫米试管,每管深度达6厘米。15磅再次灭菌15小时。) 5. Lactic-bacteria Medium I (乳酸菌培养基I ) Yeast extract (酵母膏) 7.5g Peptone (蛋白胨) 7.5g Glucose (葡萄糖) 10g KH2PO4 2g Tomato juice (西红柿汁) 100ml Tween (吐温) 80 0.5ml Distilled water (蒸馏水) 900ml pH 7.0 适用范围：植物乳杆菌(胚芽乳杆菌)、嗜热乳酸链球菌 6. Lactic-bacteria Midium Ⅱ (乳酸菌培养基Ⅱ) Lacto-casein peptone (乳酪蛋白胨) 10g Beef extract (蛋白胨) 10g Yeast extract (酵母膏) 5g Glucose (葡萄糖) 5g Tween (吐温) 80 1g K2HPO4 2g Na-acetate (醋酸钠) 5g Diamine citrate (柠檬酸二胺) 2g MgSO4.7H2O 0.2g MnSO4.H2O 0.05g Distilled water (蒸馏水) 1000m pH 6.5-6.8 适用范围：植物乳杆菌(胚芽乳杆菌) 7. Peotone Glucose Yeast extract Medium PGY (蛋白胨、酵母膏、葡萄糖培养基) Peptone(蛋白胨) 10g Yeast extract (酵母膏) 5g Glucose (葡萄糖) 1g Distilled water (蒸馏水) 1L 8. Glycerol Agar (甘油琼脂) Peptone (蛋白胨) 5g Beef extract (酵母膏) 3g Glycerol (甘油) 20g Top water (自来水) 1000ml Agar (琼脂) 15g pH 7.0-7.2 9. Rhizobium medium (根瘤菌培养基)AS 9 Yeast eztract (酵母膏) 1g Soil eztract (土壤浸提液) 200ml Mannitol (甘露醇) 10g Agar (琼脂) 15g Distilled water (蒸馏水) 800ml pH 7.2 [Note]：Soil extract：Suspend 50g finely and dried gardon soil in 200ml of tap water. Autoclave at 121℃ for 1 hr. Decant through cotton-cloth, filler though paper, make up volume to 200ml. Resterilize for 20 minutes at 121℃ , then mixed with othringredients an d distributed. (土壤浸提液的制法：取土壤50克，加水200毫升，15磅蒸煮1小时，经滤纸过滤后加水补足到200毫升。) 适用范围：大豆根瘤菌(慢生型)、豇豆慢生根瘤菌、花生根瘤菌、紫云英根瘤菌、 大豆根瘤菌(快生型)、大豆根瘤菌、豌豆根瘤菌、苜蓿根瘤菌、田菁根瘤菌 10. Mannitol Agar (甘露醇琼脂) Yeast extract (酵母膏) 5g Peptone (蛋白胨) 3g Mannitol (甘露醇) 25g Agar (琼脂) 15g Distilled water (蒸馏水) 1000ml 11. Glucose Asparagine (葡萄糖、天门冬素琼脂) Glucose (葡萄糖) 10g Asparagine (天门冬素) 0.5g K2HPO4 0.5g Water (水) 1000ml pH 7.2-7.4 适用范围：刺孢小单孢菌绛红变种、紫色小单孢菌(绛红小单孢菌) 12. Gause′s Synthetic Agar (高氏合成一号琼脂) KNO3 1g Soluble starch(可溶性淀粉) 20g K2HPO4 0.5g MgSO4.7H2O 0.5g NaCl 0.5g FeSO4 0.01g Agar (琼脂) 20g water (水) 1000ml pH 7.2-7.4 适用范围：刺孢小单孢菌绛红变种、紫色小单孢菌(绛红小单孢菌)、白黄链霉菌、白色链霉菌、抗生链霉菌、双重轮丝链霉菌、产色 链霉菌、烬灰链霉菌、天蓝色链霉菌、灭蚊链霉菌、红霉素链霉菌、青色链霉菌、球孢链霉菌、浅灰链霉菌、灰色链霉菌、吸水链霉 菌、淡紫灰链霉菌、黄色长孢链霉菌、藤黄色链霉菌、细黄链霉菌、黑化链霉菌、玫瑰色链霉菌、华美链霉菌、嗜热链霉菌、委内瑞 拉链霉菌、紫色直丝链霉菌、紫色链霉菌、绿色链霉菌 13. Wort Agar (麦芽汁琼脂) Dilute the world (without hop) to 12 Brix. Add 15g agar into 1000ml of the diluted word..Melt the agar by heating, then di stribute the medium into tubes. Autoclave at 110 for 30 minutes. (将发酵啤酒的原料(未加酒花)，稀释至12柏林，加琼脂15克， 溶化后分装。15 磅灭菌30分钟。) 适用范围：克鲁斯假丝酵母、郎比可假丝酵母、解脂假丝酵母、马其顿假丝酵母、拟热带假丝酵母、粗壮假丝酵母、皱褶假丝酵母、 热带假丝酵母、产朊假丝酵母、阿舒假囊酵母、白地霉、果香地霉、地霉属、异常汉逊酵母、异常汉逊酵母变种、阿拉伯糖醇汉逊酵 母、施氏汉逊酵母、菅囊酵母、伯顿毕赤酵母、膜醭毕赤酵母、粘红酵母、小红酵母、胶红酵母、深红酵母、卡尔斯伯酵母、酿酒酵 母、椰园酿酒酵母、发酵性酵母、洛格酵母、罗斯酵母、鲁氏酵母多形鲁氏酵母、威尔酵母、酵母、路德类酵母、栗酒裂殖酵母、掷 抱酵母、贝雷丝孢酵母、皮状丝孢酵母、糙孢曲霉、无壳曲霉、鲜橙曲霉、红曲霉、紫红曲霉、红色红曲霉、红曲霉菌 14. Potato Dextrose Agar PDA (马铃薯、葡萄糖琼脂) Potato extract (马铃薯汁) 1000ml Dextrose(glucose) (葡萄糖) 20g Agar (琼脂) 20g [Note]: Potato extract: Slice potatoes thin. Add 1000ml of water, immediately to prevent Oxidtion of juice and boil until sof t. (approximately 30min.). Filter through cotton-cloth. Autoclave at 115℃ for 20 minutes.(注：取去皮马铃薯200克，切成小块， 加水1000毫升煮沸30分钟，滤去马铃薯块，将滤液补足至1000毫升，加葡萄糖20克，琼脂15克，溶化后分装，15磅灭菌30分 钟。) 适用范围：酵米面假单胞菌(酵米面黄杆菌)、白色链霉菌、烬灰链霉菌、青色链霉 菌、球孢链霉菌、灰色链霉菌、龟裂链霉菌、伞枝梨头霉、雅致放射毛霉、棒曲霉、 米曲霉、出芽短梗霉、白僵霉、灰葡萄孢、顶头孢霉、巴西毛壳、长刺毛壳、橄榄包 毛壳、反曲毛壳、琥珀毛壳、圆酵毛壳、束状刺盘孢、新月弯孢霉、奇异翅孢壳、地 生翅孢壳、串珠镰孢、尖镰孢、盘长孢菌(鲁保一号)、银白杨盘长孢、玉蜀黍长蠕 孢、深黄被孢霉、小被孢霉、多头被孢、拉曼被孢霉、葡萄色被孢霉、生香毛霉、卷 枝毛霉、两型孢毛霉、直立毛霉、球孢毛霉、丝球毛霉、大毛霉、多型孢毛霉、总状 毛霉、鲁氏毛霉、五通桥毛霉、黑球漆斑菌、露湿漆斑菌、玫烟色拟青霉、棉铃虫拟 青霉、拟青霉、球形阜孢、白腐菌、少根根霉、华根霉、科恩根霉、戴尔根霉、日本根霉、爪哇根霉、米根霉、点头根霉、绿穂霉、 簇孢匍柄霉、雅致枝霉、康宁木霉、绿色木霉、黄萎轮枝孢、大丽花轮枝孢 15. Czapek′s Agar(察氏琼脂) Sucrose (蔗糖) 30g NaNO3 3g MgSO4.7H2O 0.5g KCl 0.5g FeSO4.7H2O 0.01g K2HPO4 1g Agar (琼脂) 13g Distilled water (蒸馏水) 1000ml pH 7.2 适用范围：红霉素链霉菌、洋葱曲霉、金头曲霉、泡盛曲霉、泡盛曲霉烟色变种、亮白曲霉、炭黑曲霉、鹿皮色曲霉、棒曲霉、无花 果曲霉、黄柄曲霉、黄曲霉、烟曲霉、构巢曲霉、黑曲霉、赭曲霉、米曲霉、寄生曲霉、酱油曲霉、土曲霉、宇佐美曲霉、焦曲霉、 杂色曲霉、温特曲霉、阿达青霉、产黄青霉、黄绿青霉、桔青霉、顶青霉、园弧青霉、绳状青霉、灰黄青霉、岛青霉、淡紫青霉、特 异青霉、赭绿青霉、产紫青霉、娄地青霉、小刺青霉、荨麻青霉、 16. Concentrated Sucrose Czapek′s (浓糖察氏琼脂) Sucrose (蔗糖) 200g NaNO3 3g MgSO4.7H2O 0.5g KCl 0.5g FeSO4.4H2O 0.01g K2HPO4 1g Agar (琼脂) 13g Distilled water (蒸馏水) 1000ml 适用范围：谢瓦曲霉 17. Synthetic Potato Medium (综合马铃薯培养基) 20%Potato extract (马铃薯汁) 1L Glucose (葡萄糖) 20g KH2PO4 3g MgSO4.7H2O 1.5g Vitanib B1 (硫胺素) Trace (微量) Agar (琼脂) 15g pH 6 适用范围：米曲霉、酱油曲霉、栖土曲霉、宇佐美曲霉、出芽短梗霉、白僵霉、灰葡萄孢、顶头孢霉、巴西毛客、长刺毛壳、球毛壳、 橄榄包毛壳、反曲毛壳、琥珀毛壳、圆酵毛壳、多主枝孢、束状刺盘孢、葫芦科刺盘孢、新月弯孢霉、奇异翅孢壳、地生翅孢壳、串 珠镰孢、蚀脉镰孢霉、盘长孢菌(鲁保一号)、银白杨盘长孢、瓶毛壳、金龟子绿僵菌、生香毛霉、卷枝毛霉、两型孢毛霉、直立毛霉、 球孢毛霉、丝球毛霉、大毛霉、多型孢毛霉、总状毛霉、鲁氏毛霉、五通桥毛霉、黑球漆斑菌、露湿漆斑菌、玫烟色拟青霉、棉铃虫 拟青霉、淡紫色拟青霉、拟青霉、白腐菌、少根根霉、根霉属的菌、华根霉、科恩根霉、戴尔根霉、台湾根霉、日本根霉、爪哇根霉、 黑根霉、雪白根霉、少根根霉、米根霉、点头根霉、三宝垄根霉、根霉属的种、绿穂霉、康宁木霉、木素木霉、拟康氏木霉、木霉、 粉红木霉、绿色木霉、黄萎轮枝孢、大丽花轮枝孢、双孢蘑菇、大肥菇、发光假蜜环菌、木耳、皱木耳、毛木耳、大秃马勃、长根菇、 金针菇、棘托竹荪、短裙竹荪、短裙竹荪东北变种(暂定)、长裙竹荪、白鬼笔、无裙竹荪、宽鳞大孔菌、凸圆灵芝(白芝)、紫芝、灵芝 (红芝，赤芝，日本灵芝，韩国灵芝)、灰树花、猴头菌、香菇、粗毛斗菇、洁丽香菇、豹皮菇、玉蕈、真姬菇、安络小皮伞菌、鬼毛 针、黑柄皮伞菌、尖顶羊肚菌、圆锥羊肚菌、高羊肚菌、黑脉羊肚菌、羊肚菌、滑菇、光帽黄伞、尖鳞黄伞、金顶側耳、榆黄磨、金 顶磨、白黄側耳、紫孢平菇、姬菇、裂皮側耳、拟囊体側耳(鲍鱼菇)、真线側耳短孢变种、真线側耳、扇形平菇、扁形平菇、佛罗里 达无孢子側耳、佛罗里达側耳、蚝菇、糙皮側耳、平菇、贝形側耳、鹰翅菌、肺形側耳、柳树菌、树窝、扁柄側耳、美味側耳、紫孢 側耳、粉褶側耳、粉红側耳、凤尾菇、漏斗状側耳、榆干側耳、大榆磨、側耳、茯苓、黑菇、烟色红菇、裂褶菌、鸡纵、金耳、黄木 耳、银耳、白木耳、紫晶口磨、银丝菇、丝盖小包脚菇、草菇 18. Corn Meal Medium (玉米粉培养基) Mix corn meal with water [20:100(W/V)] well . Distribute the mixture into 100ml bottles , each bottle contains about 30ml of the mixture . Autoclave at 121℃ for 30minutes . (20%玉米粉装瓶，15 磅灭菌30分钟。) 19. Filter Paper Medium (滤纸培养基) (NH4)2SO4 1g KH2PO4 1g MgSO4.7H2O 0.7g NaCl 0.5g Distilled water (蒸馏水) 1000ml A strip of filter paper (滤纸条) 6ⅹ1cm (厘米) 20. Soybean Cake Meal Agar (黄豆饼粉琼脂) 10% infusion of soybean cake meal (10%黄豆饼粉浸汁) 1000ml Glucose (葡萄糖) 10g NaCl 2.5g 21. Sawdust， Wheat-bran Medium (木屑、麸皮培养基) Sawdust of broad leaf tree 75g Wheat bran 25g Preparation：Mix these two ingredients well . Add appropriate water and blend (the amount of water added may be becided by kneading with fingers ). Fill the mixture into broad mouth of bottles . Stick a hole on the filling, put the cotton pulg in bottle mouth . 壳斗科植物或阔叶树的木屑75克 麸皮 25克 制法：加水适量充分混匀，水量以手捏时指间有水珠为宜，装瓶并压紧，在中心处扎一小洞，堵上棉塞，20磅灭菌1小时。 22. Pine Block or Pine Sowdust Medium (松木条或松木屑培养基) 1. Cut 1021 cm pine biocks and immerse them in 1-2% sucrose solution . Alow the biocks fully absorbing suger solution .Fill the biocks into broad month of bottle . Park the bottle with paper . Autoclave at 20 lbs for 1 hr . (将10ⅹ2ⅹ1厘米的松木条浸泡在1-2%的蔗糖 水中。使木条吸足糖水，装瓶不宜过紧，用纸包好，20磅灭菌1小时。) 2. Pine Sawdust Medium Pine Sawdust (松木屑) 75% Rice bran (米糠) 23-24% Socrose (蔗糖) 1-2% Water (appropriate)水 (适量) Autoclave at 20 lbs for 1 hr.(20 磅灭菌1小时) 23. Rice Straw. Dung Medium (稻草、马粪培养基) Dried horse dung (or cow dung) (干马粪或牛粪) 65% Died straw (干稻草) 35% Urea (尿素) 0.5% Calcium superhosphate (过磷酸钙)0.7% Gypsum powder(石膏粉) 1% Ammonium sulphate (硫酸铵) 0.3% [Note]: Add appropriate water, pile them up and cause them fermentation. (水适量进行堆积发酵) 24. Straw Rice Bran Medium (稻草、米糠培养基) Dried straw (干稻草) 75-80% Rice bran (米糠) 20-25% [Note]：Select no mouldy straw and cut them about 3 cm in length. Immerse them in fresh water for 12 hr. Allow the straw fully (the amount of water added may be decided by kneading with fingers. It is right when a drop of water appeared between fingers). ( [注]： 选无霉稻草，切成长约3 厘米的小段，用清水浸泡12小时，使充分吸水，然后倾去水，加米糠和水，水量以用手捏时指间有水珠为宜。) 25. Rice Medium (米饭培养基) Fill 10g of rice into a 50ml of Erlenmeyer flask. Add 20-30ml water. Autoclave at 121℃ for 30 minutes (or fill 2g of rice into a 15ⅹ 150mm tube). Add 6 ml of water. Autoclave at 121℃ for 30 minutes. (取大米10克，入50毫升椎形瓶中，水20-30毫升于15 磅灭 菌30分钟即成，或取大米2克，装入15ⅹ150 毫米试管中，加水6 毫升，于15磅灭菌30分钟。) 26. Filter Paper Medium (滤纸条培养基) (NH4)2SO4 1g KH2PO4 1g MgSO4.7H2O 0.5g Yeast Extract (酵母膏) 0.1g Distilled Water (蒸馏水) 1000ml Strip of filter paper 1ⅹ7 cm (滤纸条 1ⅹ7 厘米) [Note]：Siddolve the above ingredients. Distribute the liqid medium into tubes (each tube contains 1ml of the broth). Autoclave at 121 ℃ for 30 minutes. Then put one or two sterilized strips of filter paper into a tube aseptically. (将上述溶液分配成溶液后分装入试管， 每管1 毫升，15磅灭菌30分钟。再将灭菌的滤纸条(1条或2条)投入试管中即好使用。) 27. Yeast Extract-Medium Extract Agar I (酵母膏、麦芽膏琼脂) Yeast extract (酵母膏) 10g Mall extract (麦芽膏) 10g Glucose (葡萄糖) 4g Distilled water (蒸馏水) 1000ml Agar (琼脂) 20g pH 7.0 适用范围：红霉素链霉菌 28. Yeast Extract Peptone (酵母膏、蛋白胨琼脂) Yeast extract (酵母膏) 1g Multi-peptone(多蛋白胨) 2g Beef extract (牛肉膏) 1g Glucose (葡萄糖) 10g Agar (琼脂) 20g Distilled water (蒸馏水) 1L pH 7.0 |
回复此楼» 收录本帖的淘帖专辑推荐
 Nano&Micro | 分子实验常见问题 | 生物类 | 经验 |
| 鲁西微生物 |
» 猜你喜欢
288求调剂
已经有6人回复
-
复试调剂
已经有12人回复
-
计算机11408，286分求调剂
已经有3人回复
-
材料专硕322分
已经有8人回复
-
一志愿上海海洋大学083200食品学硕，求调剂，接受其他专业083200
已经有5人回复
-
081200-11408-276学硕求调剂
已经有5人回复
-
材料调剂
已经有10人回复
-
313求调剂
已经有7人回复
-
304求调剂（085602，过四级，一志愿985）
已经有14人回复
-
材料工程302分求调剂
已经有7人回复
» 本主题相关价值贴推荐，对您同样有帮助:
- TPY培养基配方
已经有3人回复
- LB培养基配方
已经有6人回复
- 【求助/交流】培养基配方看不懂
已经有6人回复
- 【求助/交流】不加碳源的无机盐培养基为什么会长很多菌？有培养基配方，小弟先谢谢了
已经有16人回复
- 【分享】培养基的配制
已经有20人回复
- 【分享】45种培养基配方及运用范围（非原创）
已经有34人回复
- 【求助/交流】求检测真菌的培养基配方！
已经有7人回复
- 【求助/交流】LB培养基的成分及成分差异的问题
已经有11人回复
|
41. Thiobacillus Ferrooxidans Medium (氧化亚铁硫杆菌培养基) (NH4)2SO4 0.15g KH2PO4 0.05g KCl 0.05g MgSO4.7H2O 0.5g Ca(NO3)2.4H2O 0.01g FeSO4.7H2O 50g Distilled water (蒸馏水) 1000ml pH 2.0 42. Thiobacillus Thiooxidans Medium (氧化硫硫杆菌培养基) (NH4)2SO4 0.3g KH2PO4 3-4g CaCl2 (anhydrous) [无水] 0.25g MgSO4.7H2O 0.5g FeSO4.7H2O 0.001g Distilled water (蒸馏水) 1000ml pH 3.5-4 43. Plasma Substitute Medium (代血浆培养基) Sucrose (蔗糖) 12.5-15% Peptone (蛋白胨) 0.5% KH2PO4 0.03% Na2HPO4.12H2O 0.14% pH 7.0-7.2 44. Skim Milk Medium (脱脂牛奶培养基) ATCC 183 脱脂牛奶100g 去离子水1000ml 适用范围：保加利亚乳杆菌、嗜热乳酸链球菌 45. Pea Agar (豌豆琼脂) Glucose (葡萄糖) 1% Peptone (蛋白胨) 0.5% NaCl 0.5% Agar (琼脂) 2.0% Pea infusion (counted by NH2) [豌豆浸汁(以NH2计)] 12-14 mg∕100 pH (before sterilization) [消前] 7.2-7.5 [Note]: Preparetion of pea infusion (豌豆浸汁的制法) : Preparetion 900g of pea. Put them into a 5L bottle, and add 3L of tap water. Adjust pH to 4.3-4.5 by using of sulphuric acid. Add 30ml of chloroform for antisepsis. Plug with rubber stopper and bind up the bottle mouth with gauge in order to avoid that the stopper fall off due to gas pressure. Place the bottle at 37℃ for 7-10 days. Filter the infusion, then boil the filtrate to escape the chloroform. Filter again Distribution and autoclave at 120℃ for 30 minutes. Store at cool contition . (称取白豌豆900克，置于5公升广口瓶中，加自 来水3 公升，用硫酸调pH至4.3-4.5，再加氯仿30毫升(防腐)，加胶塞并用纱布扎口(防止冲脱)，放37℃恒温室7-10天。取出用滤 纸过滤，滤液煮沸20分钟，以除氯仿，并再次以滤纸过滤，除沉淀。滤液分装，于120℃30分钟灭菌。后冷藏备用。) The standard quality of pea infusion as following (豌豆浸汁质量标准)： Aminonitrogen(氨基酸): 160-200 mg∕100ml Soluble phosphoruse(溶磷): 400-600 μg∕ml Color(色泽): auratus,transparent (金黄、透明) 46. Wheat Bran Medium (麦麸琼脂) Wheat bran (麦麸) 3.6% (NH4)2HPO4 0.3% K2HPO4 0.02% MgSO4.7H2O 0.01% Agar (琼脂) 2% pH 7.0 适用范围：金霉素链霉菌、红霉素链霉菌 47. Glucose Yeast Extract Agar (葡萄糖、酵母膏琼脂) Glucose (葡萄糖) 10g Yeast extract (酵母膏) 10g Water (水) 1000ml Agar (琼脂) 15g pH 7.2 48. MMN Medium (MMN培养基) CaCl2 0.05g MgSO4 0.15g NaCl 0.025g FeCl3 (1%) 1.2ml KH2PO4 0.5g Vitamib B1 (硫胺素) 100μg (NH4)2HPO4 0.25g Wort (12 Be′) 100ml Glucose (葡萄糖) 10-15g Citric acid (柠檬酸) 0.2g Distilled water (蒸馏水) 900ml pH 5.5 (左右) 适用范围：蜜环菌49. Starch Agar (淀粉琼脂) Soluble starch (可溶性淀粉) 10g NaNO3 1g MgCO3 1g K2HPO4 0.3g NaCl 0.5g Agar (琼脂) 20g Distilled water (蒸馏水) 1000ml 50. 5 Be′Wort Agar (5 Be′麦芽汁琼脂) 51. 5 Be′Koji Extract Agar (5 Be′米曲汁琼脂) 适用范围：华根霉、三宝垄根霉 52. 发根农杆菌培养基(YEB) Beef extract (牛肉浸膏) 5g/L Yeast extract (酵母膏) 1g/L Peptone (蛋白胨) 5g/L Sucrose (蔗糖) 5g/L MgSO4.7H2O 0.4g/100ml Agar (琼脂) 1.5g/100ml pH 7.4 适用范围:青枯假单胞菌 53. 青枯菌培养基(Tm) Peptone (蛋白胨) 10g Casein (干酪素水解物产) 1g Glucose (葡萄糖) 5g Ddhro (纯水) 1000ml Agar(琼脂) 15g pH 7.0 54. YM (Yeast Malt Agar) 酵母膏3.0g 麦芽汁3.0g 蛋白胨5.0g 琼脂20.0g 蒸馏水1000ml 55. ISP 4 可溶性淀粉10.0g K2HPO4 1.0g MgSO4.7H2O 1.0g NaCl 1.0g (NH4)2SO4 2.0g CaCO3 2.0g FeSO4.7H2O 0.001g MnCl2.7H2O 0.001g 琼脂 20.0g 蒸馏水1000ml 56. Trypton Soy Agar 胰蛋白胨17.0g 大豆胨3.0g 葡萄糖2.5g 琼脂20.0g NaCl 5.0g K2HPO4 2.5g 蒸馏水1000ml 57. NA (Nutrient Agar) 牛肉膏3.0g 蛋白胨5.0g 琼脂 20.0g 蒸馏水1000ml pH 6.6-7.0 58. 甘露(糖)醇琼脂(ATCC 1) 酵母膏5.0g 蛋白胨3.0g 甘露(糖)醇25.0g 琼脂15.0g 蒸馏水1000ml 59. Marine琼脂2216 (ATCC 2) 蛋白胨5.0g 酵母膏1.0g 柠檬酸铁0.1g 溴化钾0.08g NaCl 19.45g MgCl2 8.8g Na2SO4 3.24g CaCl2 1.8g KCl 0.55g NaHCO3 0.16g NH4NO3 0.0016g Na2HPO4 0.008g 氯化锶0.034g 硼酸0.022g 硅酸钠0.004g 氟化钠0.0024g 琼脂 15.0g 蒸馏水1000ml pH 7.4-7.8 |
3楼2010-04-22 13:44:26
|
60. 兔血琼脂(ATCC 4) 牛心(取其浸液) 500.0g 胰蛋白胨(Tryptose) 10.0g NaCl 5.0g 琼脂15.0g 蒸馏水1000ml pH 6.6-7.0 61. 孢子形成培养基(ATCC 5) 酵母膏1.0g 牛肉膏1.0g 胰蛋白胨2.0g 葡萄糖10.0g FeSO4 微量琼脂 15.0g 蒸馏水1000ml pH 7.2 62. Trypticase Soy Agar (ATCC 18) Trypticase Soy Broth 30.0g 琼脂15.0g 蒸馏水1000ml 63. YPAD (ATCC 109) 酵母膏10.0g 蛋白胨20.0g 葡萄糖20.0g 琼脂20.0g 蒸馏水1000ml pH 7.0 64. SP (ATCC 432) 棉子糖1.0g 蔗糖1.0g 半乳糖1g 可溶性淀粉5g 水解酪蛋白2.5g 琼脂20.0g MgSO4.7H2O 0.5g K2HPO4 0.25g 蒸馏水1000ml pH 自然 65. 胰酪胨大豆酵母浸膏琼脂(TSA-YE) 胰蛋白胨15.5g 大豆蛋白胨5.0g NaCl 5.0g 酵母膏6.5g 琼脂 15.0g 蒸馏水1000ml pH 7.3 66. ATCC 20 蛋白胨5.0g 酵母膏15.0g K2HPO4 3.0g 葡萄糖2.0g 琼脂 20.0g 蒸馏水1000ml pH 7.3-7.5 67. TY (Yeast Tryptone Agar) 蛋白胨10.0g 酵母膏5.0g 葡萄糖1.0g NaCl 5.0g 琼脂 20.0g 蒸馏水1000ml pH 7.0 68. 硫醇(Thiol)培养基(ATCC 49) 示蛋白胨(Proteose Peptone No.3,Difco) 10.0g 酵母膏5.0g 葡萄糖1.0g NaCl 5.0g 硫醇复合物8.0g 琼脂1.0g p-氨基苯甲酸0.05g pH 6.9-7.3 69. Czapek＇Dox 琼脂培养基(ATCC 134) Czapek＇Dox Broth 35.0g 琼脂15.0g 蒸馏水1000ml 70. MG/L 蛋白胨5.0g 甘露醇5.0g 谷氨酸钠1.15g 生物素0.0001g K2HPO4 0.25g NaCl 0.1g MgSO4.7H2O 0.1g 酵母膏2.5g 琼脂 20.0g 蒸馏水1000ml 氯霉素(终浓度) 50μg/ml pH 7.0 71. Eclb Broth 蛋白胨10.0g 酵母膏5.0g NaCl 5.0g 琼脂20.0g 蒸馏水1000ml 氨苄青霉素(终浓度) 300μg/ml pH 7.0 72. Bennetts Agar (IFO 228) 酵母膏1.0g 牛肉膏1.0g NZ Amine,type A 2.0g 葡萄糖10.0g 琼脂 20.0g 蒸馏水1000ml pH 7.3 73. Harroldˊs M40Y (ATCC 319) 麦芽膏20.0g 酵母膏5.0g 蔗糖400.0g 琼脂20.0g 蒸馏水1000ml 74. PSA (Potato Sucrose Agar) 土豆200g 蔗糖20g 蒸馏水1000ml 琼脂20g pH 6.0-6.5 75. 培养基 牛肉膏300g 水解酪蛋白17.5g 可溶性淀粉1.5g 琼脂20.0g 蒸馏水1000ml 磺胺嘧啶 (终浓度) 100μg/ml 氨苄青霉素 (终浓度) 100μg/ml pH 7.3-7.4 76. 马铃薯牛肉汁培养基 麦芽糖20.0g 牛肉膏5.0g 蛋白胨3.0g 马铃薯200.0g pH 7.0-7.2 77. ISP 2 酵母膏4.0g 麦芽汁10.0g 葡萄糖4.0g 琼脂20.0g 蒸馏水1000ml pH 7.0 78. BHI (Brain Heart Infusion) 牛脑200.0g 牛心浸出汁250.0g 蛋白胨10.0g 葡萄糖2.0g NaCl 5.0g 琼脂20.0g 蒸馏水1000ml pH 6.8-7.2 79. YPG (Yeast Peptone Glucose) 酵母膏10.0g 蛋白胨20.0g 葡萄糖20.0g 琼脂20.0g 蒸馏水1000ml pH 7.0 |
4楼2010-04-22 13:44:48
|
80. 兔食琼脂培养基(ATCC 340) 兔食(商店出售) 25.0g 琼脂20.0g 蒸馏水1000ml *煮沸兔食，并浸泡1/2小时，用多层纱布过滤，加琼脂于滤液中。 81. 土豆琼脂(ATCC 423) 200 克去皮土豆切成小块于1000ml 自来水中煮沸1 小时,用细棉布过滤.加进5.0mgMnSO4,pH 调至6.8,用自来水定容至1000ml。加 进15.0g琼脂，溶化，分装并灭菌。 82. 土豆浸汁加无机盐培养基(ATCC 470) 土豆2000g MnSO4.5H2O 0.02g MgSO4.7H2O 0.4g ZnSO4.7H2O 0.01g CuSO4. 5H2O 0.01g FeSO4.7H2O 0.1g CaCl2.2H2O 0.1g K2HPO4 0.5g * 称取200g去皮块状土豆,加入1000ml 蒸馏水煮沸30分钟,多层纱布过滤,滤液中加入以上成分,溶解后定容至1000ml。 83. 无机盐淀粉琼脂培养基(ATCC 527) 可溶性淀粉10.0g K2HPO4 1.0g MgSO4 1.0g NaCl 1.0g (NH4)2SO4 2.0g CaCO3 2.0g FeSO4.7H2O 0.001g MnCl2.7H2O 0.001g ZnSO4.7H2O 0.001g 琼脂20.0g pH 7.0-7.4 84. (AS125) 新鲜鸡蛋15-16 蒸馏水375ml 2%孔雀(石)绿10.0g K4HPO4 1.5g MgSO4.7H2O 0.375g 天冬酰胺2.25g 甘油 7.5ml 土豆粉18.8g 85. 燕麦片(Oatmeal)琼脂培养基 燕麦片60.0g 琼脂12.5g 蒸馏水1000ml * 将燕麦片加600ml水中，制成匀浆，加热至45-50oC，然后加入已溶化琼脂的另400ml水，121oC灭菌90分钟。 86. 细菌培养基(ATCC 573) (NH4)2SO4 1.3g K2HPO4 0.37g MgSO4.7H2O 0.25g CaCl2.2H2O 0.07g FeCl3 0.02g 葡萄糖1.0g 酵母膏1.0g 蒸馏水1000ml 用10N H2SO4 调pH 至4.0。若配制固体培养基，为增强上述溶液的强度，pH 需调至3.5，琼脂水溶液浓度为40g/L。两者分别高压 灭菌，冷却至约50oC，无菌操作将两种溶液等体积混合，这一过程可避免琼脂被酸水解。 87. 碎肉培养基(ATCC 593) 牛肉(去脂肪) 500.0g 蒸馏水1000ml NaOH 25.0ml * 所用瘦牛肉或马肉(碾)磨碎前需去脂肪和周围的组织。将肉、水、NaOH混合后边搅拌边加热，冷至室温，去掉表面油脂，过滤，留 下肉末和滤液。滤液加蒸馏水至1000ml。滤液中加下列成分： 蛋白胨30.0g 酵母膏5.0g K2HPO4 5.0g 0.025%刃天青(Resazurin)溶液4.0ml 然后煮沸，冷却，加0.5g L-半胱氨酸HCl。H2O，pH调至7.0。在氧气、氮气(O2-freenitrogen)和3%氢气条件下，按1份碎肉加4-5 份以上溶液的比例分装于每支试管。在氮气和氢气条件下，使用橡皮塞，快排气，高压灭菌15分钟。 88. YMPG 酵母膏3.0g 麦芽汁3.0g 蛋白胨5.0g 葡萄糖10.0g FeSO4.7H2O 0.1g 琼脂20.0g 蒸馏水1000ml pH 6.8 灭菌：110oC，35分钟 89. TGYM培养基(ATCC 679) 胰蛋白胨5.0g 葡萄糖1.0g 酵母膏3.0g 蒸馏水1000ml DL-蛋氨酸(DL-Meth 0.5g 90. TYG培养基(ATCC 741) 胰蛋白胨3.0g 酵母膏3.0g 葡萄糖3.0g K2HPO4 1.0g 琼脂 20.0g 蒸馏水1000ml pH 7.4 91. M9 Na2HPO4 12.8g KH2PO4 3.0g NaCl 0.5g NH4Cl 0.5g 葡萄糖(单独灭菌) 10.0g 维生素B1(单独灭菌) 1μg/ml pH 自然 92. ISP3 (IFO 245) 燕麦粉20.0g 微量元素溶液1ml pH 7.2 微量元素： FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g ZnSO4.7H2O 0.1g 93. Oatmeal Agar 琼脂 5.0g 蒸馏水500ml 加热溶化速溶燕麦粉40.0g 蒸馏水250ml 加热溶化后与琼脂液混合加水至1000ml pH 5.8 94. V8-Juice Agar (AYCC 343) V-8 果汁200g CaCO3 3.0g 琼脂 20.0g 蒸馏水1000ml pH 5.8 95. Czapek-Dox Agar (ATCC 312) NaNO3 3.0g K2HPO4 1.0g MgSO4.7H2O 0.5g KCl 0.5g FeSO4.7H2O 0.01g 蔗糖(单独灭菌) 30.0g 琼脂 20.0g 蒸馏水1000ml pH 5.6 A 液 琼脂 5.0g GC琼脂培养基(BBL 11275) 36.0g 蒸馏水500.0ml B 液 干燥色牛血红蛋白(BBL 11871) 10.0g 蒸馏水500.0ml Iso Vitale X (BBL 11876) 10.0ml 97. 固氮螺菌培养基(ATCC 838) K2HPO4 0.1g KH2PO4 0.4g MgSO4.7H2O 0.2g NaCl 0.1g CaCl2 0.02g FeCl3 0.01g NaMoO4.2H2O 0.002g 苹果酸钠(Na malate) 5.0g 酵母膏0.05g 蒸馏水1000ml pH 7.2-7.4 98. BG-11 NaNO3 1.5g K2HPO4 0.04g MgSO4.7H2O 0.075g CaCl2.7H2O 0.036g Na2CO3 0.02g 柠檬酸0.006g 柠檬酸铁0.006g *微量元素溶液A5 1ml 氨苄青霉素 (终浓度) 50μg/ml 蒸馏水1000ml 琼脂 20.0g * 微量元素溶液A5 H3BO4 2.86g MnCl2.4H2O 1.81g ZnSO4 0.222g Na2MoO4 0.39g CuSO4.5H2O 0.079g Co(NO3)2.6H2O 49.4g 99. (Marine Agar) 蛋白胨5.0g 酵母膏1.0g 柠檬酸铁0.1g NaCl 19.45g MgSO4.7H2O 5.9g KCl 0.55g Na2SO4 3.24g CaCl2 1.8g NaHCO3 0.16g KI 0.08g 微量元素10ml pH 7.0-7.6 * 微量元素溶液： 氯化锶0.034g 硅酸钠0.004g 蒸馏水1000ml H3BO4 0.022g NaCl 0.0024g NaNO3 0.0016g NaHPO4 0.008g 蒸馏水1000ml 100.(Yeast Starch Agar) 酵母膏2.0g 可溶性淀粉10.0g 琼脂 20.0g 蒸馏水1000ml pH 7.2-7.4 101.GYMC 葡萄糖4.0g 酵母膏2.0g 麦芽汁20.0g 琼脂20.0g CaCO3 1.5g 蒸馏水1000ml pH 6.8-7.0 102. LCSB 乳糖15.0g 玉米浆5.0g 蛋白胨5.0g NaCl 4.0g MgSO4.7H2O 0.5g KH2PO4 0.6g FeCl3.6H2O 0.005g CuSO4.5H2O 0.002g 琼脂 20.0g 蒸馏水1000ml 相对湿度65% pH 4.8 103.高氏1号(G) 可溶性淀粉20.0g KNO3 1.0g K2HPO4 0.5g MgSO4.7H2O 0.5g NaCl 0.5g FeSO4.7H2O 0.01g 琼脂 20.0g 蒸馏水1000ml pH 7.0-7.2 104.葡萄糖-酵母膏琼脂(ATCC 846) 酵母膏5.0g 蛋白胨1.0g KH2SO4 1.0g NaCl 1.0g 琼脂 20.0g 蒸馏水950.0ml pH 6.8 补加50ml10%葡萄糖(葡萄糖溶液单独过滤灭菌)。 105.Tep尿液(Uric Acid)琼脂(ATCC 894) Na2HPO4.12H2O 9.0g KH2PO4 1.5g MgSO4.7H2O 0.2g 柠檬酸铁铵(绿色) 1.2g CaCl2 20.0mg MnCl2.4H2O 1.0mg Trypticcase Soy Broth (BBL 11768) 3.0g 尿素4.0g 琼脂 20.0 蒸馏水1000ml pH 7.2 106.增强梭菌生长的培养基(ATCC 1053) 胰蛋白胨10.0g 牛肉膏10.0g 酵母膏3.0g 葡萄糖5.0g NaCl 5.0g 可溶性淀粉1.0g 半胱氨酸盐酸盐0.5g 醋酸钠3.0g 琼脂 15.0g 蒸馏水1000ml pH 6.6-7.0 107.酵母膏-甘露(糖)醇琼脂(ATCC 1205) K2HPO4 0.5g MgSO4.7H2O 0.2g NaCl 0.1g 甘露(糖)醇10.0g 酵母膏0.4g 蒸馏水加至1000ml 108.Tomato Dextrin Agar (ATCC 965) 番茄汁20.0g 糊精20.0g 酵母膏10.0g CoCl2.6H2O 5.0mg 琼脂 20.0g 蒸馏水1000ml pH 7.2-7.4 109.嗜热菌无机盐溶液(ATCC 1554) FeSO4 1.0mg MgSO4.7H2O 200.0mg Na2HPO4 210.0mg NaH2PO4 90.0mg KCl 40.0mg CaCl2 15.0mg NaNO3 0.25g NH4Cl 0.25g 微量元素溶液(见下面) 10.0ml n-Heptadecane(C17) 0.1%(v/v) 蒸馏水1000ml 微量元素溶液： CuSO4.5H2O 500mcg H3BO4 1.0mg MnSO4.5H2O 7.0mcg ZnSO4.7H2O 7.0mg MoO3 1.0mg CoSO4.7H2O 18.0mcg 蒸馏水1000ml 110.醋杆菌培养基(AS 1) 葡萄糖100.0g 酵母膏10.0g CaCO3 20.0g 琼脂15.0g 蒸馏水1000ml pH 6.8 适用范围：园弧青霉、少根根霉、日本根霉、爪哇根霉 |
5楼2010-04-22 13:45:16
★ ★ ★
lstt09nk(金币+3):太长了，看完后眼有点晕~~ 2010-04-22 18:25
lstt09nk(金币+3):太长了，看完后眼有点晕~~ 2010-04-22 18:25
|
29. Glucose Yeast Extract Starch Agar (???????????????????) Glucose (??????) 10g Yeast extract (?????) 10g Starch (????) 10g NaCl 5g CaCO3 3g Agar (???) 20g Distilled water (?????) 1L pH 7.0 30. Wheat Bran Medium (?????????) Wheat bran (???) 36g (NH4)2HPO4 10g K2HPO4 0.2g MgSO4??7H2O 0.1g Agar (???) 20g Distilled water (?????) 1L pH 7.0 31. Yeast Sporulation Medium (???????????????????????) (1) Kleyn Medium KH2PO4 0.012% K2HPO4 0.02% Sodium acetate (??????) 0.5% Glucose (??????) 0.062% NaCl 0.062% Peptone (??????) 0.25% Biotin (??????) 2??g% Agar (???) 2% Trace element solution (???????) 1ml/100ml [Note]: Trace element solution (??????????)?? MgSO4.7H2O 0.4%, NaCl 0.4%, CuSO4.5H2O 0.002%, MnSO4.4H2O 0.2%, FeSO4.4H2O 0.2% (2) Gorodkowa Medium Glucose (??????) 0.1% Peptone (??????) 1% NaCl 0.5% Agar (???) 2% (3) McClary Medium Glucose (??????) 0.1% KCl 0.18% Yeast juice (????) 0.25% Sodium actate (??????) 0.82% Agar (???) 1.5% 32. BPY Medium (BPY ??????) Beef extract (????) 5g Prptone (??????) 10g NaCl 5g Yeast extract (?????) 5g Glucose (??????) 5g Agar (???) 1-2% Distilled (?????) 1000ml pH 7.0 ???÷?Χ?????????(????)???????????? 33. LB Medium (LB ??????) Yeast extact (?????) 5g Peptone (??????) 10g NaCl 10g Agar (???) 1-2% Distilled water (?????) 1000ml pH 7.0 ???÷?Χ?????????(????) 34. Penssay Broth (Penssay ????) Beef extract (????) 1.5g Yeast extract (?????) 1.5g Tryptone (???????) 5g NaCl 3.5g Glucose (??????) 1g K2HPO4.3H2O 4.8g Distilled water (?????) 1000ml pH 7.2 35. Blond Agar (??????????) Peptone (??????) 10g Glucose (??????) 2g NaCl 5g NaH2PO4 2.5g Sodium citrate (????????) 5g Brain infusion (????) 800ml Pig heart infusion (?????) 200ml Sleep Blood (???) 10% pH 7.2 [Note]??Preparation of pig brain and pig heart infusion?? (1) Smash up 100g fresh pig heart (without fat and blood vessel).Add distilled water up to 1000ml; (2) Cut 200g fresh pig brain (without blood vessel). Add distilled water up to 1000ml; (3) Keep the two infusions at 50?? for 1 hr , then boil for 15 minutes; (4) Cool and filtrate through gauze . [?]???????????????(1)????????????????????????100??????????1000??????(2) ???????????????200 ??????????1000??????(3)50?汣??1С????????15?????(4)????????????????á? 36. Cellulose??decomposing Bacteria Synthetic Medium (???????????????) NaCl 6g MgSO4.7H2O 0.1g KH2PO4 0.5g CaCl2 0.1g K2HPO4 2.0g Yeast extract (?????) 1.0g (NH4)2SO4 2.0g Distilled water (?????) 1000ml Filter paper strip: one strip (?????????) pH 7.0-7.4 [Note]: So;id medium for cellulose-decomposing bacteria: Carboxymethyl Cellulose 2% Agar 2%. (б????????????????2%????? 2%???????????CMCNa ) 37. Clostridium Pasreurianum Synthetic Medium (?????????????????????) Glucose (??????) 1% MnSO4 0.001% KH2PO4 0.05% FeSO4 0.001% K2HPO4 0.05% Yeast extract(?????) 0.1% MgSO4.7H2O 0.02% Peptone (??????) 0.01% NaCl 0.001% CaCO3 0.5% Distille water (?????) 100% pH 7.0 38. Yeast Extract-Medium Extract Agar ?? (???????????????) Yeast extract (?????) 4g Mall extract (?????) 10g Glucose (??????) 4g Agar (???) 17g Distilled water (????) 1000ml pH 7.3 39. Oatemeal Agar (????????) Oatemeal(infusion)[???????] 20g Agar (???) 18% Trace element solution (?????????)1ml Distilled water up to (?????????????1000 ????) 1000ml pH 7.2 [Note]??Trace element solution (?????????)?? FeSO4.7H2O 0.1g MnCl2.4H2O 0.1g ZnSO4.7H2O 0.1g Distilled water (?????) 100ml40. Calcium Malate Agar (?????????) Calium malate (??????) 10g K2HPO4 0.5g NH4Cl 0.5g Glycerol (????) 10g Agar (???) 15g Distilled water (?????) 1000ml |
2楼2010-04-22 13:44:01
6楼2010-04-22 17:07:53
7楼2010-04-22 18:30:51
8楼2010-06-20 19:25:05
9楼2010-09-11 20:14:07
10楼2010-09-11 20:33:19
11楼2010-09-12 00:27:07
12楼2010-09-12 09:03:07
|
本帖内容被屏蔽 |
13楼2010-09-27 10:01:47
14楼2010-10-02 17:16:55
15楼2010-10-02 23:49:17
16楼2010-10-17 17:08:29
|
本帖内容被屏蔽 |
17楼2010-11-06 21:34:02
18楼2010-11-29 11:25:12
19楼2010-11-29 16:25:22
20楼2010-12-02 13:00:28
21楼2010-12-02 13:08:37
24楼2011-09-26 16:00:13
25楼2011-09-29 14:37:29
27楼2012-03-15 16:13:01
28楼2012-06-03 09:09:14
31楼2012-09-17 12:12:28
32楼2013-03-01 09:38:16
34楼2013-04-26 22:54:32
35楼2014-01-09 23:19:06
36楼2014-04-09 19:11:47
37楼2014-05-29 10:22:37
40楼2015-01-04 16:44:59
41楼2015-06-16 13:51:11
42楼2017-09-21 07:36:31
43楼2018-03-12 09:47:00
|
本帖内容被屏蔽 |
44楼2018-12-15 01:29:38
|
本帖内容被屏蔽 |
45楼2021-10-16 21:22:46
简单回复
shf52512022楼
2010-12-02 20:43
回复
shf52512023楼
2010-12-02 20:44
回复
adhkl佩26楼
2011-10-30 16:06
回复
三星好评 谢谢分享
bobby310429楼
2012-06-03 11:09
回复
五星好评 
guyue131430楼
2012-08-30 19:49
回复
五星好评 
hp01133楼
2013-03-01 16:38
回复
五星好评
chshen0618838楼
2014-11-27 09:05
回复
chshen0618839楼
2014-11-27 09:06
回复
五星好评 
