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churmi

银虫 (正式写手)

[交流] PCR条件优化求助!

如题:
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Tamplates are metagenome from soils.

Target products are 200bp of 16s rDNA, generated from metagenome of various microbes from soil.

Although the size is correct, brightness is not high enough.

So I want to know how to optimize my program?

by increasing the cycle times or ......
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churmi

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94 for 3min
94 for 45s
57 for 30s
72 for 45s
...
3楼2009-11-15 22:04:13
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yzhang1986

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you should show your condition
2楼2009-11-15 21:15:39
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zhouzhou_3006

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churmi(金币+1,VIP+0): 11-19 22:39
57 for 30 s, you can use a lower temperature and different concentrations of template. Good luck
为梦想努力。
4楼2009-11-17 22:31:30
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