Znn3bq.jpeg
ÉÇÍ·´óѧº£Ñó¿ÆÑ§½ÓÊܵ÷¼Á
²é¿´: 433  |  »Ø¸´: 4
µ±Ç°Ö÷ÌâÒѾ­´æµµ¡£

churmi

Òø³æ (ÕýʽдÊÖ)

[½»Á÷] PCRÌõ¼þÓÅ»¯ÇóÖú£¡

ÈçÌ⣺
       The products are showed as follow£º

Tamplates are metagenome from soils.

Target products are 200bp of 16s rDNA, generated from metagenome of various microbes from soil.

Although the size is correct, brightness is not high enough.

So I want to know how to optimize my program?

by increasing the cycle times or ......
»Ø¸´´ËÂ¥

» ²ÂÄãϲ»¶

ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

yzhang1986

Ìú¸Ëľ³æ (ÎÄ̳¾«Ó¢)

ÓÅÐã°æÖ÷

you should show your condition
2Â¥2009-11-15 21:15:39
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

churmi

Òø³æ (ÕýʽдÊÖ)

94 for 3min
94 for 45s
57 for 30s
72 for 45s
...
3Â¥2009-11-15 22:04:13
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

zhouzhou_3006

ľ³æ (ÖøÃûдÊÖ)

¡ï
churmi(½ð±Ò+1,VIP+0): 11-19 22:39
57 for 30 s£¬ you can use a lower temperature and different concentrations of template. Good luck
ΪÃÎÏëŬÁ¦¡£
4Â¥2009-11-17 22:31:30
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû

fykxy_206

ľ³æ (ÕýʽдÊÖ)

¡ï
churmi(½ð±Ò+1,VIP+0): 11-19 22:39
2nd PCR
5Â¥2009-11-17 22:49:50
ÒÑÔÄ   »Ø¸´´ËÂ¥   ¹Ø×¢TA ¸øTA·¢ÏûÏ¢ ËÍTAºì»¨ TAµÄ»ØÌû
Ïà¹Ø°æ¿éÌø×ª ÎÒÒª¶©ÔÄÂ¥Ö÷ churmi µÄÖ÷Ìâ¸üÐÂ
×î¾ßÈËÆøÈÈÌûÍÆ¼ö [²é¿´È«²¿] ×÷Õß »Ø/¿´ ×îºó·¢±í
[¿¼ÑÐ] 327Çóµ÷¼Á +23 Xxjc1107. 2026-04-13 25/1250 2026-04-14 18:48 by åÐÒ£ÈýÀÉ
[¿¼ÑÐ] 290Çóµ÷¼Á +21 luoziheng 2026-04-10 23/1150 2026-04-14 15:49 by zs92450
[¿¼ÑÐ] 271Çóµ÷¼Á +35 2261744733 2026-04-11 41/2050 2026-04-14 15:36 by zs92450
[¿¼ÑÐ] Çóµ÷¼Á +16 Ñ©·ê¶¬ 2026-04-10 16/800 2026-04-14 14:27 by ÄæË®³Ë·ç
[¿¼ÑÐ] 293Çóµ÷¼Á +16 ÎÒ°®¸ßÊý¸ßÊý°®Î 2026-04-12 18/900 2026-04-13 21:47 by ѧԱJpLReM
[¿¼ÑÐ] һ־Ը³¶«´óѧ071000ÉúÎïѧѧ˶³õÊÔ·ÖÊý276Çóµ÷¼Á +8 Ľ¾øcc 2026-04-09 8/400 2026-04-13 14:08 by ÕÅzhihao
[¿¼ÑÐ] ²ÄÁÏ¿¼Ñе÷¼Á +29 ÔÆÄ¾´ï´ï 2026-04-11 31/1550 2026-04-13 13:32 by lyh³ÀÏʦ
[¿¼ÑÐ] ÉúÎïѧµ÷¼Á +11 СȽҪŬÁ¦ 2026-04-10 13/650 2026-04-13 11:46 by µç»¯Ñ§¼°´ß»¯
[¿¼ÑÐ] 0831Ò»ÂÖµ÷¼Áʧ°ÜÇóÖú +10 СÐÜî£î£_s 2026-04-11 10/500 2026-04-12 22:43 by ³¤¹­°Á
[½Ìʦ֮¼Ò] ɽ¶«Ë«·ÇԺУ¿¼ºË³¬¼¶ÎÞµ×Ïߣ¬Áìµ¼ÐÒÔÖÀÖ»ö£¬½ÌʦÔâÑê¿Ö +3 qut2026 2026-04-11 7/350 2026-04-12 20:24 by qut2026
[¿¼ÑÐ] 22408 352·ÖÇóµ÷¼Á +5 ŬÁ¦µÄÏÄÄ© 2026-04-09 5/250 2026-04-12 19:17 by wj165256
[¿¼ÑÐ] 086003µ÷¼ÁÇóÖú +21 ËÕß®Íò 2026-04-09 22/1100 2026-04-11 20:25 by dongdian1
[¿¼ÑÐ] 085501»úеר˶ 302·Ö ²»ÌôרҵÇóµ÷¼Á +7 Íôij. 2026-04-09 7/350 2026-04-11 14:37 by luhong1990
[¿¼ÑÐ] ũѧ0904 312Çóµ÷¼Á +6 Say Never 2026-04-10 6/300 2026-04-11 10:33 by wwj2530616
[¿¼ÑÐ] 083200 305·Ö Çó¶þÂÖµ÷¼Á ²»½ÓÊÜ¿çרҵ +9 Claireyyyy 2026-04-09 10/500 2026-04-10 21:21 by Claireyyyy
[ÂÛÎÄͶ¸å] mdpiСÐÞrvrʱ¼äËÄÎåÌìÁË 20+3 ¹þ¹þhigh 2026-04-08 5/250 2026-04-10 16:02 by ±±¾©À³ÒðÈóÉ«
[¿¼ÑÐ] µ÷¼Á +12 ÔÂ@163.com 2026-04-08 12/600 2026-04-09 14:27 by rl1980
[¿¼ÑÐ] 085801 ×Ü·Ö275 ±¾¿ÆÐÂÄÜÔ´ Çóµ÷¼Á +8 bradoner 2026-04-08 9/450 2026-04-09 13:43 by onlyÖÜ
[¿¼ÑÐ] ¶þ´Îµ÷¼ÁÇóÀÏʦÊÕÁô +3 ЦЦԬ 2026-04-08 3/150 2026-04-08 23:50 by ×íÔÚ·çÀï
[¿¼ÑÐ] ÉúÎïѧѧ˶£¬³õÊÔ351·Ö£¬Çóµ÷¼Á +4 ¡­¡«¡¢Íõ¡­¡« 2026-04-08 5/250 2026-04-08 21:49 by limeifeng
ÐÅÏ¢Ìáʾ
ÇëÌî´¦ÀíÒâ¼û