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churmi

Òø³æ (ÕýʽдÊÖ)

[½»Á÷] PCRÌõ¼þÓÅ»¯ÇóÖú£¡

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Tamplates are metagenome from soils.

Target products are 200bp of 16s rDNA, generated from metagenome of various microbes from soil.

Although the size is correct, brightness is not high enough.

So I want to know how to optimize my program?

by increasing the cycle times or ......
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yzhang1986

Ìú¸Ëľ³æ (ÎÄ̳¾«Ó¢)

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you should show your condition
2Â¥2009-11-15 21:15:39
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churmi

Òø³æ (ÕýʽдÊÖ)

94 for 3min
94 for 45s
57 for 30s
72 for 45s
...
3Â¥2009-11-15 22:04:13
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zhouzhou_3006

ľ³æ (ÖøÃûдÊÖ)

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churmi(½ð±Ò+1,VIP+0): 11-19 22:39
57 for 30 s£¬ you can use a lower temperature and different concentrations of template. Good luck
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4Â¥2009-11-17 22:31:30
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fykxy_206

ľ³æ (ÕýʽдÊÖ)

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churmi(½ð±Ò+1,VIP+0): 11-19 22:39
2nd PCR
5Â¥2009-11-17 22:49:50
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