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whn732
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zhangwj(½ð±Ò+1,VIP+0):thanks 6-24 23:37
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zhangwj(½ð±Ò+1,VIP+0):thanks 6-24 23:37
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Determination of free amine groups [37]. ¼ì²â°±»ùµÄ Dried gelatin samples (2¨C4 mg) were reacted with TNBS (0.01 M) in a sodium hydrogencarbonate solution (pH 8.2, 4 w/v%, 2 ml) for 2 h at 40¡ÀC. Then hydrochloric acid (6 M, 3 ml) was added to hydrolyse the gelatin gels at 60¡ÀC for 1.5 h. After cooling to room temperature, deionised water (5 ml) was added to the solution and the absorbance was measured against a TNBS-solution without gelatin, which had been treated exactly the same as the other samples, at ¸ 345 nmwith an Uvikon 930 spectrometer. The amount of free amine groups per 1000 amino acids was calculated using the absorption coefŽ cient for 2,4,6-trinitrophenyl derivatised (hydroxy)lysine residues (" D 14 600 lmol¡1 cm¡1 ), and assuming a molecular weight of 105 g mol¡1 for a gelatin molecular chain of 1000 amino acids. The experiments were performed in triplicate. [ Last edited by whn732 on 2009-6-24 at 23:36 ] |
2Â¥2009-06-24 23:35:44
whn732
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zhangwj(½ð±Ò+1,VIP+0):thanks 6-24 23:38
Þ¹Ò²ݶù(½ð±Ò+1,VIP+0):ºÜÇ¿´ó£¬ÔÙ¼ÓÒ»¸ö~:) 9-6 11:53
zhangwj(½ð±Ò+1,VIP+0):thanks 6-24 23:38
Þ¹Ò²ݶù(½ð±Ò+1,VIP+0):ºÜÇ¿´ó£¬ÔÙ¼ÓÒ»¸ö~:) 9-6 11:53
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Titration of free carboxylic acid groups. ¼ì²âôÈ»ùµÄ The titration of the cross-linked gels was carried out using a protein titration procedure according to Kenchington et al. [38, 39]. This procedure was adapted for titration of small volumes of insoluble materials. The gelatin gels were swollen in deionised water for 1 h, powdered and lyophilised. Deionised water (1.5 ml) as such or containing powdered gelatin gel (50 mg) at 40¡ÀC was either titrated with hydrochloric acid (0.2 M) until pH 1.5 or with sodium hydroxide (0.2 M) until pH 12, by adding 0.005 ml per step, and an equilibration time of 60 s per addition. |
3Â¥2009-06-24 23:36:26
whn732
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zhangwj(½ð±Ò+1,VIP+0):лл¹ËÎÊ¡«¡« 6-24 23:38
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Ïà¹ØÎÄÏ× J. Biomater. Sci. Polymer Edn, Vol. 11, No. 3, pp. 225¨C243 (2000) Cross-linking and characterisation of gelatin matrices for biomedical applications ALMA J. KUIJPERS 1, GERARD H. M. ENGBERS1, JEROEN KRIJGSVELD2, SEBASTIAN A. J. ZAAT 2, JACOB DANKERT2 and JAN FEIJEN1;¡è |
4Â¥2009-06-24 23:37:54
5Â¥2009-06-25 13:45:34
iceinwater
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yusen_1982(½ð±Ò+1,VIP+0):»¶ÓÌÖÂÛ! 6-25 23:38
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7Â¥2009-07-21 07:46:00
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8Â¥2009-07-28 10:25:04
bighead7288
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9Â¥2009-09-05 22:30:12
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