| 查看: 1202 | 回复: 11 | |||
| 当前主题已经存档。 | |||
[交流]
【求助】用什么检测水凝胶的羧基和氨基含量
|
|||
| 如题，水凝胶做出来后，是网络状固体，不溶于任何溶剂。能有什么方法检测水凝胶中的氨基和羧基含量？不管什么方法都行。 |
回复此楼» 猜你喜欢
大连工业大学 生物质能源与材料专业招收调剂生，有兴趣加联系：15732893021
已经有0人回复
-
学科生物354求调剂
已经有0人回复
-
生物力学与组织工程学论文润色/翻译怎么收费?
已经有92人回复
-
大连工业大学 生物质能源与材料专业招收调剂生，有兴趣加联系：15732893021
已经有0人回复
-
生物光子学研究院/激光生命科学教育部重点实验室 研究生调剂
已经有0人回复
-
大连工业大学 生物质能源与材料专业招收调剂生，有兴趣加联系：15732893021
已经有0人回复
-
求调剂！生物与医药专硕
已经有5人回复
-
大连工业大学 生物质能源与材料专业招收调剂生，有兴趣加联系：15732893021
已经有0人回复
-
大连工业大学 生物质能源与材料专业招收调剂生，有兴趣将简历发送到507432328@qq.com
已经有0人回复
-
生物学308分求调剂（一志愿华东师大）
已经有7人回复
-
大连工业大学 生物质能源与材料专业招收调剂生，有兴趣将简历发送到507432328@qq.com
已经有0人回复
★ ★
zhangwj(金币+1,VIP+0):thanks 6-24 23:37
薰衣草儿(金币+1,VIP+0):很强大，再加一个~:) 9-6 11:53
zhangwj(金币+1,VIP+0):thanks 6-24 23:37
薰衣草儿(金币+1,VIP+0):很强大，再加一个~:) 9-6 11:53
|
Determination of free amine groups [37]. 检测氨基的 Dried gelatin samples (2–4 mg) were reacted with TNBS (0.01 M) in a sodium hydrogencarbonate solution (pH 8.2, 4 w/v%, 2 ml) for 2 h at 40±C. Then hydrochloric acid (6 M, 3 ml) was added to hydrolyse the gelatin gels at 60±C for 1.5 h. After cooling to room temperature, deionised water (5 ml) was added to the solution and the absorbance was measured against a TNBS-solution without gelatin, which had been treated exactly the same as the other samples, at ¸ 345 nmwith an Uvikon 930 spectrometer. The amount of free amine groups per 1000 amino acids was calculated using the absorption coefŽ cient for 2,4,6-trinitrophenyl derivatised (hydroxy)lysine residues (" D 14 600 lmol¡1 cm¡1 ), and assuming a molecular weight of 105 g mol¡1 for a gelatin molecular chain of 1000 amino acids. The experiments were performed in triplicate. [ Last edited by whn732 on 2009-6-24 at 23:36 ] |
2楼2009-06-24 23:35:44
★ ★
zhangwj(金币+1,VIP+0):thanks 6-24 23:38
薰衣草儿(金币+1,VIP+0):很强大，再加一个~:) 9-6 11:53
zhangwj(金币+1,VIP+0):thanks 6-24 23:38
薰衣草儿(金币+1,VIP+0):很强大，再加一个~:) 9-6 11:53
|
Titration of free carboxylic acid groups. 检测羧基的 The titration of the cross-linked gels was carried out using a protein titration procedure according to Kenchington et al. [38, 39]. This procedure was adapted for titration of small volumes of insoluble materials. The gelatin gels were swollen in deionised water for 1 h, powdered and lyophilised. Deionised water (1.5 ml) as such or containing powdered gelatin gel (50 mg) at 40±C was either titrated with hydrochloric acid (0.2 M) until pH 1.5 or with sodium hydroxide (0.2 M) until pH 12, by adding 0.005 ml per step, and an equilibration time of 60 s per addition. |
3楼2009-06-24 23:36:26
★ ★ ★ ★ ★ ★
zhangwj(金币+1,VIP+0):谢谢顾问～～ 6-24 23:38
吃茶去(金币+5,VIP+0):谢谢，我会认真看看这篇文章的 6-28 21:06
zhangwj(金币+1,VIP+0):谢谢顾问～～ 6-24 23:38
吃茶去(金币+5,VIP+0):谢谢，我会认真看看这篇文章的 6-28 21:06
|
相关文献 J. Biomater. Sci. Polymer Edn, Vol. 11, No. 3, pp. 225–243 (2000) Cross-linking and characterisation of gelatin matrices for biomedical applications ALMA J. KUIJPERS 1, GERARD H. M. ENGBERS1, JEROEN KRIJGSVELD2, SEBASTIAN A. J. ZAAT 2, JACOB DANKERT2 and JAN FEIJEN1;¤ |
4楼2009-06-24 23:37:54
5楼2009-06-25 13:45:34
6楼2009-06-25 13:56:06
7楼2009-07-21 07:46:00
8楼2009-07-28 10:25:04
bighead7288
木虫 (小有名气)
大将军
- 应助: 0 (幼儿园)
- 金币: 1805.1
- 红花: 1
- 帖子: 249
- 在线: 32.1小时
- 虫号: 690918
- 注册: 2009-01-10
- 性别: GG
- 专业: 功能与智能高分子
9楼2009-09-05 22:30:12
yusen_1982
荣誉版主 (职业作家)
小木虫最高学位委员会主席
- 应助: 7 (幼儿园)
- 贵宾: 3.738
- 金币: 24009.8
- 散金: 279
- 红花: 5
- 沙发: 1
- 帖子: 3849
- 在线: 477.3小时
- 虫号: 398312
- 注册: 2007-06-10
- 性别: GG
- 专业: 金属功能材料
- 管辖: 金属
10楼2009-09-06 06:17:03
