| 查看: 1232 | 回复: 11 | |||
| 当前主题已经存档。 | |||
| 当前只显示满足指定条件的回帖，点击这里查看本话题的所有回帖 | |||
[交流]
【求助】用什么检测水凝胶的羧基和氨基含量
|
|||
| 如题，水凝胶做出来后，是网络状固体，不溶于任何溶剂。能有什么方法检测水凝胶中的氨基和羧基含量？不管什么方法都行。 |
回复此楼» 猜你喜欢
08开头
已经有0人回复
-
中科中山药物创新研究院（药创院）怎么样？
已经有0人回复
-
生物物理、生物化学与分子生物学论文润色/翻译怎么收费?
已经有229人回复
-
4月19日晚八点前填系统 广西医科大学医学生物化学与分子生物学招生信息
已经有3人回复
★ ★ ★ ★ ★ ★
zhangwj(金币+1,VIP+0):谢谢顾问～～ 6-24 23:38
吃茶去(金币+5,VIP+0):谢谢，我会认真看看这篇文章的 6-28 21:06
zhangwj(金币+1,VIP+0):谢谢顾问～～ 6-24 23:38
吃茶去(金币+5,VIP+0):谢谢，我会认真看看这篇文章的 6-28 21:06
|
相关文献 J. Biomater. Sci. Polymer Edn, Vol. 11, No. 3, pp. 225–243 (2000) Cross-linking and characterisation of gelatin matrices for biomedical applications ALMA J. KUIJPERS 1, GERARD H. M. ENGBERS1, JEROEN KRIJGSVELD2, SEBASTIAN A. J. ZAAT 2, JACOB DANKERT2 and JAN FEIJEN1;¤ |
4楼2009-06-24 23:37:54
★ ★
zhangwj(金币+1,VIP+0):thanks 6-24 23:37
薰衣草儿(金币+1,VIP+0):很强大，再加一个~:) 9-6 11:53
zhangwj(金币+1,VIP+0):thanks 6-24 23:37
薰衣草儿(金币+1,VIP+0):很强大，再加一个~:) 9-6 11:53
|
Determination of free amine groups [37]. 检测氨基的 Dried gelatin samples (2–4 mg) were reacted with TNBS (0.01 M) in a sodium hydrogencarbonate solution (pH 8.2, 4 w/v%, 2 ml) for 2 h at 40±C. Then hydrochloric acid (6 M, 3 ml) was added to hydrolyse the gelatin gels at 60±C for 1.5 h. After cooling to room temperature, deionised water (5 ml) was added to the solution and the absorbance was measured against a TNBS-solution without gelatin, which had been treated exactly the same as the other samples, at ¸ 345 nmwith an Uvikon 930 spectrometer. The amount of free amine groups per 1000 amino acids was calculated using the absorption coefŽ cient for 2,4,6-trinitrophenyl derivatised (hydroxy)lysine residues (" D 14 600 lmol¡1 cm¡1 ), and assuming a molecular weight of 105 g mol¡1 for a gelatin molecular chain of 1000 amino acids. The experiments were performed in triplicate. [ Last edited by whn732 on 2009-6-24 at 23:36 ] |
2楼2009-06-24 23:35:44
★ ★
zhangwj(金币+1,VIP+0):thanks 6-24 23:38
薰衣草儿(金币+1,VIP+0):很强大，再加一个~:) 9-6 11:53
zhangwj(金币+1,VIP+0):thanks 6-24 23:38
薰衣草儿(金币+1,VIP+0):很强大，再加一个~:) 9-6 11:53
|
Titration of free carboxylic acid groups. 检测羧基的 The titration of the cross-linked gels was carried out using a protein titration procedure according to Kenchington et al. [38, 39]. This procedure was adapted for titration of small volumes of insoluble materials. The gelatin gels were swollen in deionised water for 1 h, powdered and lyophilised. Deionised water (1.5 ml) as such or containing powdered gelatin gel (50 mg) at 40±C was either titrated with hydrochloric acid (0.2 M) until pH 1.5 or with sodium hydroxide (0.2 M) until pH 12, by adding 0.005 ml per step, and an equilibration time of 60 s per addition. |
3楼2009-06-24 23:36:26
5楼2009-06-25 13:45:34