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protein2008

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[交流] G Protein Signaling: Methods and Protocols

G Protein Signaling: Methods and Protocols (Methods in Molecular Biology) by Alan V. Smrcka

Publisher: Humana Press | September 15, 2003 | ISBN: 1588291375 | Pages: 264 | PDF | 2 MB


http://www.filefactory.com/file/ag39d22/n/GProSig_rar
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protein2008

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Signal Transduction Protocols (Methods in Molecular Biology)

Signal Transduction Protocols (Methods in Molecular Biology) by Robert C. Dickson
Publisher: Humana Press | 2 edition (June 18, 2004) | ISBN: 1588292452 | Pages: 327 | PDF | 2.21 MB

Carrying on the high standards of the much-acclaimed first edition, highly experienced investigators have extensively updated the first edition with many of the new approaches that have been transforming the field. Included in this new edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class.

http://www.filefactory.com/file/ag46568/n/SignaTran_rar
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protein2008

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Transmembrane signaling protocols, 1st Ed

Transmembrane signaling protocols, 1st Ed, by Dafna Bar-Sagi
Publisher: Springer | 1998 | ISBN: 0896034321 | Pages: 328 | PDF | 22 MB

This collection of practical, cutting-edge techniques for the study of cell signaling provides detailed, step-by-step instructions, helpful notes, and troubleshooting tips that make even the most powerful of the newest techniques readily reproducible. The protocols presented include the use of peptide libraries to study transmembrane signaling; the use of single-cell assays to analyze signal transduction pathways; the reconstitution of signaling complexes; methods for analyzing protein-protein interactions, and more. Introductory reviews explain the basic theory and enable researchers new to the area to rapidly gain understanding, as well as command of the practical knowledge and expertise afforded by the protocols. Transmembrane Signaling Protocols makes available to all researchers the many state-of-the-art techniques that have recently led to landmark discoveries in transmembrane signaling.

http://www.filefactory.com/file/ag39f89/n/TraSig_rar
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protein2008

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Redox-Mediated Signal Transduction: Methods and Protocols”

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[ Last edited by protein2008 on 2009-6-7 at 00:46 ]
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protein2008

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Redox-Mediated Signal Transduction: Methods and Protocols”

John T. Hancock, “Redox-Mediated Signal Transduction: Methods and Protocols”
Humana Press | 2008 | ISBN: 1588298426 | 234 pages | PDF | 2,8 MB

Once believed to be involved mainly with energetics, including the production of ATP, knowledge of the role of redox in the control of cellular activity has been expanded over recent years. In Redox-Mediated Signal Transduction: Methods and Protocols, experienced researchers with backgrounds in both the plant and animal sciences contribute timely methods and techniques that can be used to study this important aspect of biology. Beginning with an overview and methods for measuring compounds that affect redox and the redox state of cells, the book continues with reviews of the use of GFP and its derivatives, methods to study the impact of changing redox on proteins, and methods to study the exact molecular changes that may underlie the mechanisms of action of altering redox, among other subjects. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include step-by-step, readily reproducible protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Redox-Mediated Signal Transduction: Methods and Protocols is an ideal reference for those who wish to enter this exciting area of research as well as for those who simply wish for a more thorough understanding of the dramatic impact of redox in the control of cellular function

http://depositfiles.com/en/files/5kjxucgq0
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protein2008

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David M. Terrian , "Cancer Cell Signaling Methods and Protocols (Methods in Molecular Biology)"
Humana Press; 1 edition | ISBN: 1588290751 | 352 pages | PDF | 3,2 Mb

"...this volume is a very useful source for signal transduction protocols...This text should offer readers an excellent starting point for many important techniques in cancer signal transduction. Its ease of use and reasonable cost only add to its attraction." - Neuro-Oncology

Book Description
A collection of readily reproducible experimental methods for studying the subversion of normal cell signaling that leads to cancer. Presented in step-by-step detail to ensure successful results, the methods include proven techniques for the investigation of apoptosis and cell death, complementary protocols for manipulating and/or monitoring oncogenic signals in cancer cells, and techniques for studying protein-protein interactions. A detailed protocol for capturing pure samples of malignant cells from frozen tissue specimens and two alternative techniques for analyzing their genomic DNA is also described.

http://depositfiles.com/en/files/2045474
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protein2008

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Transmembrane Signaling Protocols-2nd Edition (Methods in Molecular Biology volume 332)
Humana Press | ISBN:158829546X | 2006-02-21 | 376 Pages | 3.2MB | Rapidshare&Megaupload

This fully updated and expanded second edition of Transmembrane Signaling Protocols demonstrates the use of such techniques as single cell imaging, bioluminecence resonance energy transfer, and global proteomics in the study of transmembrane signaling events. Highlights include the functional expression and genetic selection of mutant mammalian transmembrane receptors in yeast, real-time analysis of transmembrane receptor signaling in live cells, and bioluminescence energy transfer to monitor protein-protein interaction. Additional chapters use state-of-the-art methods to analyze gene expression and proteomic profiles related to transmembrane signaling events, as well as to genetically reconstitute bone marrow for the study of signal transduction ex vivo. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.


http://rapidshare.com/files/5945 ... ling_Protocols-.rar

Pass: nguyenhuy

"

[ Last edited by protein2008 on 2009-6-7 at 00:56 ]
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rokiyou

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小木虫(金币+0.5):给个红包，谢谢回帖交流
顶一下，呵呵，不知道怎么下，能传到纳米盘吗
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695

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贼好的资源
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雪山飞狐7233

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铁杆小虫
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有的不能下载，楼主能传到纳米盘就好了！
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梦在路就在！
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