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protein2008

至尊木虫 (知名作家)

[交流] G Protein Signaling: Methods and Protocols

G Protein Signaling: Methods and Protocols (Methods in Molecular Biology) by Alan V. Smrcka

Publisher: Humana Press | September 15, 2003 | ISBN: 1588291375 | Pages: 264 | PDF | 2 MB


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protein2008

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Transmembrane Signaling Protocols-2nd Edition (Methods in Molecular Biology volume 332)
Humana Press | ISBN:158829546X | 2006-02-21 | 376 Pages | 3.2MB | Rapidshare&Megaupload

This fully updated and expanded second edition of Transmembrane Signaling Protocols demonstrates the use of such techniques as single cell imaging, bioluminecence resonance energy transfer, and global proteomics in the study of transmembrane signaling events. Highlights include the functional expression and genetic selection of mutant mammalian transmembrane receptors in yeast, real-time analysis of transmembrane receptor signaling in live cells, and bioluminescence energy transfer to monitor protein-protein interaction. Additional chapters use state-of-the-art methods to analyze gene expression and proteomic profiles related to transmembrane signaling events, as well as to genetically reconstitute bone marrow for the study of signal transduction ex vivo. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.


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[ Last edited by protein2008 on 2009-6-7 at 00:56 ]
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protein2008

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Signal Transduction Protocols (Methods in Molecular Biology)

Signal Transduction Protocols (Methods in Molecular Biology) by Robert C. Dickson
Publisher: Humana Press | 2 edition (June 18, 2004) | ISBN: 1588292452 | Pages: 327 | PDF | 2.21 MB

Carrying on the high standards of the much-acclaimed first edition, highly experienced investigators have extensively updated the first edition with many of the new approaches that have been transforming the field. Included in this new edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class.

http://www.filefactory.com/file/ag46568/n/SignaTran_rar
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protein2008

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Transmembrane signaling protocols, 1st Ed

Transmembrane signaling protocols, 1st Ed, by Dafna Bar-Sagi
Publisher: Springer | 1998 | ISBN: 0896034321 | Pages: 328 | PDF | 22 MB

This collection of practical, cutting-edge techniques for the study of cell signaling provides detailed, step-by-step instructions, helpful notes, and troubleshooting tips that make even the most powerful of the newest techniques readily reproducible. The protocols presented include the use of peptide libraries to study transmembrane signaling; the use of single-cell assays to analyze signal transduction pathways; the reconstitution of signaling complexes; methods for analyzing protein-protein interactions, and more. Introductory reviews explain the basic theory and enable researchers new to the area to rapidly gain understanding, as well as command of the practical knowledge and expertise afforded by the protocols. Transmembrane Signaling Protocols makes available to all researchers the many state-of-the-art techniques that have recently led to landmark discoveries in transmembrane signaling.

http://www.filefactory.com/file/ag39f89/n/TraSig_rar
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protein2008

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Redox-Mediated Signal Transduction: Methods and Protocols”

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[ Last edited by protein2008 on 2009-6-7 at 00:46 ]
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