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protein2008至尊木虫 (知名作家)
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G Protein Signaling: Methods and Protocols
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G Protein Signaling: Methods and Protocols (Methods in Molecular Biology) by Alan V. Smrcka Publisher: Humana Press | September 15, 2003 | ISBN: 1588291375 | Pages: 264 | PDF | 2 MB http://www.filefactory.com/file/ag39d22/n/GProSig_rar |
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protein2008
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Redox-Mediated Signal Transduction: Methods and Protocols”
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John T. Hancock, “Redox-Mediated Signal Transduction: Methods and Protocols” Humana Press | 2008 | ISBN: 1588298426 | 234 pages | PDF | 2,8 MB Once believed to be involved mainly with energetics, including the production of ATP, knowledge of the role of redox in the control of cellular activity has been expanded over recent years. In Redox-Mediated Signal Transduction: Methods and Protocols, experienced researchers with backgrounds in both the plant and animal sciences contribute timely methods and techniques that can be used to study this important aspect of biology. Beginning with an overview and methods for measuring compounds that affect redox and the redox state of cells, the book continues with reviews of the use of GFP and its derivatives, methods to study the impact of changing redox on proteins, and methods to study the exact molecular changes that may underlie the mechanisms of action of altering redox, among other subjects. As a volume in the highly successful Methods in Molecular Biology™ series, chapters include step-by-step, readily reproducible protocols, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, Redox-Mediated Signal Transduction: Methods and Protocols is an ideal reference for those who wish to enter this exciting area of research as well as for those who simply wish for a more thorough understanding of the dramatic impact of redox in the control of cellular function http://depositfiles.com/en/files/5kjxucgq0 |
5楼2009-06-07 00:45:45
protein2008
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Signal Transduction Protocols (Methods in Molecular Biology)
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Signal Transduction Protocols (Methods in Molecular Biology) by Robert C. Dickson Publisher: Humana Press | 2 edition (June 18, 2004) | ISBN: 1588292452 | Pages: 327 | PDF | 2.21 MB Carrying on the high standards of the much-acclaimed first edition, highly experienced investigators have extensively updated the first edition with many of the new approaches that have been transforming the field. Included in this new edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class. http://www.filefactory.com/file/ag46568/n/SignaTran_rar |
2楼2009-06-07 00:36:58
protein2008
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Transmembrane signaling protocols, 1st Ed
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Transmembrane signaling protocols, 1st Ed, by Dafna Bar-Sagi Publisher: Springer | 1998 | ISBN: 0896034321 | Pages: 328 | PDF | 22 MB This collection of practical, cutting-edge techniques for the study of cell signaling provides detailed, step-by-step instructions, helpful notes, and troubleshooting tips that make even the most powerful of the newest techniques readily reproducible. The protocols presented include the use of peptide libraries to study transmembrane signaling; the use of single-cell assays to analyze signal transduction pathways; the reconstitution of signaling complexes; methods for analyzing protein-protein interactions, and more. Introductory reviews explain the basic theory and enable researchers new to the area to rapidly gain understanding, as well as command of the practical knowledge and expertise afforded by the protocols. Transmembrane Signaling Protocols makes available to all researchers the many state-of-the-art techniques that have recently led to landmark discoveries in transmembrane signaling. http://www.filefactory.com/file/ag39f89/n/TraSig_rar |
3楼2009-06-07 00:41:18
protein2008
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Redox-Mediated Signal Transduction: Methods and Protocols”
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(duplicate) [ Last edited by protein2008 on 2009-6-7 at 00:46 ] |
4楼2009-06-07 00:44:29
