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protein2008

至尊木虫 (知名作家)

[交流] G Protein Signaling: Methods and Protocols

G Protein Signaling: Methods and Protocols (Methods in Molecular Biology) by Alan V. Smrcka

Publisher: Humana Press | September 15, 2003 | ISBN: 1588291375 | Pages: 264 | PDF | 2 MB


http://www.filefactory.com/file/ag39d22/n/GProSig_rar
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protein2008

至尊木虫 (知名作家)

David M. Terrian , "Cancer Cell Signaling Methods and Protocols (Methods in Molecular Biology)"
Humana Press; 1 edition | ISBN: 1588290751 | 352 pages | PDF | 3,2 Mb

"...this volume is a very useful source for signal transduction protocols...This text should offer readers an excellent starting point for many important techniques in cancer signal transduction. Its ease of use and reasonable cost only add to its attraction." - Neuro-Oncology

Book Description
A collection of readily reproducible experimental methods for studying the subversion of normal cell signaling that leads to cancer. Presented in step-by-step detail to ensure successful results, the methods include proven techniques for the investigation of apoptosis and cell death, complementary protocols for manipulating and/or monitoring oncogenic signals in cancer cells, and techniques for studying protein-protein interactions. A detailed protocol for capturing pure samples of malignant cells from frozen tissue specimens and two alternative techniques for analyzing their genomic DNA is also described.

http://depositfiles.com/en/files/2045474
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6楼2009-06-07 00:47:03
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protein2008

至尊木虫 (知名作家)

Signal Transduction Protocols (Methods in Molecular Biology)

Signal Transduction Protocols (Methods in Molecular Biology) by Robert C. Dickson
Publisher: Humana Press | 2 edition (June 18, 2004) | ISBN: 1588292452 | Pages: 327 | PDF | 2.21 MB

Carrying on the high standards of the much-acclaimed first edition, highly experienced investigators have extensively updated the first edition with many of the new approaches that have been transforming the field. Included in this new edition are readily reproducible immunoassays, fluorescence-based assays, high-throughput methods, protein modification assays, lipid second messenger assays, and chromatin immunoprecipitation techniques. Wherever possible, protocols for the assay of general classes of signal transduction components have been identified so they can be adapted to the assay of any member of that class.

http://www.filefactory.com/file/ag46568/n/SignaTran_rar
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2楼2009-06-07 00:36:58
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protein2008

至尊木虫 (知名作家)

Transmembrane signaling protocols, 1st Ed

Transmembrane signaling protocols, 1st Ed, by Dafna Bar-Sagi
Publisher: Springer | 1998 | ISBN: 0896034321 | Pages: 328 | PDF | 22 MB

This collection of practical, cutting-edge techniques for the study of cell signaling provides detailed, step-by-step instructions, helpful notes, and troubleshooting tips that make even the most powerful of the newest techniques readily reproducible. The protocols presented include the use of peptide libraries to study transmembrane signaling; the use of single-cell assays to analyze signal transduction pathways; the reconstitution of signaling complexes; methods for analyzing protein-protein interactions, and more. Introductory reviews explain the basic theory and enable researchers new to the area to rapidly gain understanding, as well as command of the practical knowledge and expertise afforded by the protocols. Transmembrane Signaling Protocols makes available to all researchers the many state-of-the-art techniques that have recently led to landmark discoveries in transmembrane signaling.

http://www.filefactory.com/file/ag39f89/n/TraSig_rar
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3楼2009-06-07 00:41:18
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protein2008

至尊木虫 (知名作家)

Redox-Mediated Signal Transduction: Methods and Protocols”

(duplicate)

[ Last edited by protein2008 on 2009-6-7 at 00:46 ]
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4楼2009-06-07 00:44:29
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