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为什么内切酶识别回文序列？究竟什么是回文序列？那位跟我解释一下？ @biostar2009 发自小木虫Android客户端 |
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2楼2016-11-05 11:55:57
【答案】应助回帖
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The sequence of enzyme digestion site usually a sequence in which the same sequence shows in both direction strands. Or restriction site. Restriction sites, or restriction recognition sites, are locations on a DNA molecule containing specific (4-8 base pairs in length) sequences of nucleotides, which are recognized by restriction enzymes. These are generally palindromic sequences (because restriction enzymes usually bind as homodimers), and a particular restriction enzyme may cut the sequence between two nucleotides within its recognition site, or somewhere nearby. For example, the common restriction enzyme EcoRI recognizes the palindromic sequence GAATTC and cuts between the G and the A on both the top and bottom strands, leaving an overhang (an end-portion of a DNA strand with no attached complement) known as a sticky end on each end of AATT. This overhang can then be used to ligate in (see DNA ligase) a piece of DNA with a complementary overhang (another EcoRI-cut piece, for example). Some restriction enzymes cut DNA at a restriction site in a manner which leaves no overhang, called a blunt end. |
3楼2016-11-05 12:47:49
