| 查看: 743 | 回复: 2 | ||
[求助]
酪氨酸酶活性实验
|
|
我最近在做酪氨酸酶活性实验，参考文献方法做出来的数据不知道怎么分析，请大家帮忙看看，文献上说酶的活力是这样计算的：Relative enzymatic activity (%) = (slope of reaction kinetics equation obtained by reaction with inhibitor)/(slope of reaction kinetics equation obtained by reaction without inhibitor) *100%，其中方法是这样：The enzyme activity was determined at room temperature by monitoring the absorbance at 475 nm accompanying the oxidation of substrate L-DOPA (Chen & Kubo, 2002). A series of assay solutions dissolved in 0.05 mol L1 sodium phosphate buffer (pH 6.8) containing various amount of morin and a fixed concentration of tyrosinase (5.21 10 8 mol L1) were incubated for 3 h at room temperature. The assay was initiated by adding L-DOPA (levodopa, 5.0 104 mol L1) to the morin–tyrosinase complex solution (the final volume of the reaction system was 3.0 mL), and the absorbance at 475 nm was then measured every 20 s. Kojic acid was used as a positive control. The enzymatic relative activity assayed without morin was defined as 100%. 我不明白酶活力的计算公式中的两个斜率是什么意思？请大家帮我分析分析，多谢！@biostar2009@飞约疯人院@wizardfan@youlinglyw |
回复此楼» 猜你喜欢
Wnt-C59：PORCN靶向化学探针，Wnt信号通路阻断机制与科研应用全解析
已经有0人回复
-
Fexaramine：肠道限制性FXR化学探针，结构特征与信号通路研究全解析
已经有0人回复
-
化学工程及工业化学论文润色/翻译怎么收费?
已经有109人回复
-
NVS-STG2：靶向STING跨膜域的分子胶类激动剂，结构特征与寡聚化机制研究全解析
已经有0人回复
-
DOTA-GPC3肽（CAS 3066439-94-6）：双功能分子探针前体的结构特征与标记应用解析
已经有0人回复
-
科研用 TLR7/8 双激动剂怎么选？现货与质控要点
已经有0人回复
-
SEL120-34A HCl：CDK8/CDK19高选择性激酶抑制剂
已经有0人回复
-
氢氧化钾活化操作
已经有18人回复
-
散金
已经有68人回复
-
生物质超级电容器
已经有0人回复
2楼2016-04-13 17:46:37
3楼2016-04-25 08:24:37
20
