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ÎÄÕÂÐÅÏ¢ÈçÏ£º You Li, Dongwei Mao, Guoda Ma, Lili Cui, Hua Tao, Haihong Zhou, Wandong Liang, Bin Zhao,and Keshen Li Nuclear export of VP19C is not essential for replication of herpes simplex virus type 1. Cell Biosci. 2014; 4: 55.Published online 2014 Sep 19. doi: 10.1186/2045-3701-4-55 PMCID: PMC4190327 лл¡£ |
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Nuclear export of VP19C is not essential for replication of herpes simplex virus type 1 ×÷Õß:Li, Y (Li, You)[ 1,3 ] ; Mao, DW (Mao, Dongwei)[ 2 ] ; Ma, GD (Ma, Guoda)[ 1 ] ; Cui, LL (Cui, Lili)[ 1 ] ; Tao, H (Tao, Hua)[ 5 ] ; Zhou, HH (Zhou, Haihong)[ 5 ] ; Liang, WD (Liang, Wandong)[ 4 ] ; Zhao, B (Zhao, Bin)[ 1,5 ] ; Li, KS (Li, Keshen)[ 1 ] Òþ²Ø ResearcherID ºÍ ORCID²é¿´ ResearcherID ºÍ ORCID ×÷Õß ResearcherID ORCID ºÅ tao, hua https://orcid.org/0000-0002-9506-5987 CELL AND BIOSCIENCE ¾í: 4 ÎÄÏ׺Å: 55 DOI: 10.1186/2045-3701-4-55 ³ö°æÄê: SEP 19 2014 ²é¿´ÆÚ¿¯ÐÅÏ¢ CELL AND BIOSCIENCE ³ö°æÉÌ BIOMED CENTRAL LTD, 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND ISSN: 2045-3701 Ñо¿ÁìÓò Biochemistry & Molecular Biology ÕªÒª Background: Herpes simplex virus (HSV) type 1 has a 152 kb double-stranded DNA genome that may encode more than 80 gene products, many of which remain uncharacterized. The HSV-1 triplex is a complex of three protein subunits, VP19C and a dimer of VP23 that is essential for capsid assembly. Previous studies have demonstrated that HSV-1 VP19C contains an atypical nuclear localization signal and a functional nuclear export signal (NES), which are both important for the nucleocytoplasmic shuttling of VP19C. However, whether the VP19C NES is required for efficient HSV-1 production is unknown. Findings: In the present study, a VP19C NES-mutated recombinant virus was generated by using bacterial artificial chromosome recombineering technology to investigate the role of VP19C nuclear export in HSV-1 replication. Our results demonstrate that the growth curves, plaque areas, subcellular localization and viral gene expression are indistinguishable between the VP19C NES-mutated virus and the wild-type virus. Conclusions: Our findings reported herein indicate abrogation of the nuclear export of VP19C did not affect HSV-1 replication and viral gene expression. ¹Ø¼ü´Ê ×÷Õ߹ؼü´Ê:VP19C; Nuclear export signal (NES); Bacterial artificial chromosome (BAC); Replication KeyWords Plus:LOCALIZATION; IDENTIFICATION; SIGNALS; HSV-1 ×÷ÕßÐÅÏ¢ ͨѶ×÷ÕßµØÖ·: Zhao, B (ͨѶ×÷Õß) Affiliated Hosp, Guangdong Med Coll, Guangdong Key Lab Age Related Cardiac & Cerebral, Zhanjiang 524001, Peoples R China. µØÖ·: [ 1 ] Affiliated Hosp, Guangdong Med Coll, Guangdong Key Lab Age Related Cardiac & Cerebral, Zhanjiang 524001, Peoples R China [ 2 ] Harbin Med Univ, Affiliated Hosp 4, Dept Obstet & Gynaecol, Harbin 150001, Heilongjiang, Peoples R China ÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ Harbin Medical University [ 3 ] Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan 430071, Peoples R China ÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ Chinese Academy of Sciences [ 4 ] Wenzhou Med Univ, Renji Coll, Wenzhou 325000, Peoples R China ÔöÇ¿×éÖ¯ÐÅÏ¢µÄÃû³Æ Wenzhou Medical University [ 5 ] Affiliated Hosp, Guangdong Med Coll, Dept Neurol, Zhanjiang 524001, Peoples R China µç×ÓÓʼþµØÖ·:zhaobine@126.com; likeshen1971@126.com »ù½ð×ÊÖúÖÂл »ù½ð×ÊÖú»ú¹¹ ÊÚȨºÅ National Nature Science Foundation of China 31171219 81271213 81070878 81271214 81300929 81261120404 ²é¿´»ù½ð×ÊÖúÐÅÏ¢¹Ø±Õ»ù½ð×ÊÖúÐÅÏ¢ This work was supported by funding from the National Nature Science Foundation of China (grant numbers 31171219, 81271213, 81070878, 81271214, 81300929 and 81261120404). We thank Dr. Frazer J. Rixon, Dr. C. Sweet, Dr. David Leib, Dr. Gregory Smith and Dr. Nikolaus Osterrieder for the generous gift of the antibodies against VP19C and VP23, the antibody against VP22, 17-37BAC, E. coli GS1783 and pEPkan-S, respectively. ³ö°æÉÌ BIOMED CENTRAL LTD, 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND Àà±ð / ·ÖÀà Ñо¿·½Ïò:Biochemistry & Molecular Biology Web of Science Àà±ð:Biochemistry & Molecular Biology ÎÄÏ×ÐÅÏ¢ ÎÄÏ×ÀàÐÍ:Article ÓïÖÖ:English Èë²ØºÅ: WOS:000343817500001 PubMed ID: 25302111 ISSN: 2045-3701 ÆäËûÐÅÏ¢ IDS ºÅ: AR8HZ Web of Science ºËÐĺϼ¯ÖÐµÄ "ÒýÓõIJο¼ÎÄÏ×": 10 Web of Science ºËÐĺϼ¯ÖÐµÄ "±»ÒýƵ´Î": 0 JCR® Àà±ð JCR ·ÖÇø BIOCHEMISTRY & MOLECULAR BIOLOGY Q2 Êý¾ÝÀ´×ÔµÚ 2014 °æ Journal Citation Reports® ÒýÎÄÍøÂç 0 ±»ÒýƵ´Î 10 ÒýÓõIJο¼ÎÄÏ× ²é¿´ Related Records ²é¿´ÒýÖ¤¹ØÏµÍ¼ ´´½¨ÒýÎĸú×Ù (Êý¾ÝÀ´×Ô Web of ScienceTM ºËÐĺϼ¯) È«²¿±»ÒýƵ´Î¼ÆÊý 0 / ËùÓÐÊý¾Ý¿â 0 / Web of Science ºËÐĺϼ¯ 0 / BIOSIS Citation Index 0 / Öйú¿ÆÑ§ÒýÎÄÊý¾Ý¿â 0 / Data Citation Index 0 / Russian Science Citation Index 0 / SciELO Citation Index ʹÓôÎÊý ×î½ü 180 Ìì: 3 2013 ÄêÖÁ½ñ: 6 ½øÒ»²½Á˽⠴˼ǼÀ´×Ô: Web of ScienceTM ºËÐĺϼ¯ ÔÚÆäËûÊý¾Ý¿âÖв鿴¼Ç¼: ²é¿´ ÉúÎïѧÊý¾Ý (ÔÚ BIOSIS Citation IndexSM ÖÐ) ²é¿´ ÉúÎïѧÊý¾Ý (ÔÚ Biological Abstracts® ÖÐ) ²é¿´ ҽѧÊý¾Ý (ÔÚ MEDLINE® ÖÐ) ½¨ÒéÐÞÕý Èç¹ûÏ£ÍûÌá¸ß´Ë¼Ç¼ÖÐÊý¾ÝµÄÖÊÁ¿£¬ÇëÌṩÐÞÕý½¨Òé¡£ ÆÚ¿¯ÐÅÏ¢ µÚ 1 Ìõ£¬¹² 1 Ìõ Impact Factor (Ó°ÏìÒò×Ó): Journal Citation Reports® ³ö°æÉÌ´¦µÄÈ«ÎÄ NCBI ÇëÌṩÄúµÄ InCites ƾ֤¹Ø±Õ µÇ¼ÒÔÔÚ Web of Science Öб£´æ µÇ¼ÒÔÔÚ Web of Science Öдò¿ª ÇëÌṩÄúµÄ InCites ƾ֤ µç×ÓÓʼþµØÖ·: ÃÜÂë: È¡Ïû ±£³ÖµÇ¼״̬ Íü¼ÇÃÜÂë ×¢²á ±£´æÖÁ±¾µØ´ÅÅÌ ±£´æ¼ìË÷ÀúÊ·ÖÁ±¾µØ´ÅÅÌ¡£±£´æºó£¬¹Ø±Õ´Ë´°¿Ú¡£ ´Ó±¾µØ´ÅÅÌ´ò¿ª ʹÓà "ä¯ÀÀ" ´ò¿ª±¾µØ±£´æµÄ¼ìË÷ÀúÊ·Îļþ¡£ ÇëÑ¡ÔñÓÐЧµÄ±£´æµÄ¼ìË÷ÀúÊ·Îļþ¡£ ¹Ø±Õ ÇëÌṩÄúµÄ InCites ƾ֤™ µç×ÓÓʼþ: ÃÜÂë: Íü¼ÇÃÜÂë? 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